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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB10501 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB10501, RRID:AB_11188565
- Product name
- ACTB monoclonal antibody, clone 8H10D10
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against synthetic peptide of ACTB.
- Isotype
- IgG
- Antibody clone number
- 8H10D10
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using ACTB monoclonal antibody, clone 8H10D10 (Cat # MAB10501) against NIH/3T3 (1), Jurkat (2), HeLa (3), CHO (4), PC-12 (5), HEK293 (6), COS (7), A-549 (8) and MCF-7 (9) cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis of SK-BR-3 (upper) and A-549 (bottom) cells using ACTB monoclonal antibody, clone 8H10D10 (Cat # MAB10501) (red, the secondary Ab is Cy3-Goat anti mouse IgG). Blue: DRAQ5 fluorescent DNA dye.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MCF-7 cells using ACTB monoclonal antibody, clone 8H10D10 (Cat # MAB10501) (A) and negative control (B).
- Validation comment
- Flow Cytometry
