Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB600-503AF488 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal beta-Actin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat, Chicken/Avian, Porcine, Rabbit, Simian
- Host
- Rabbit
- Conjugate
- Green dye
- Isotype
- IgG
- Vial size
- 0.05 ml
- Storage
- Store at 4C in the dark.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: beta-Actin Antibody [Alexa Fluor® 488] [NB600-503AF488] - An intracellular stain was performed on HeLa cells with beta-Actin Antibody NB600-503AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.