Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- ABIN950261 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Actin, beta (ACTB) antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein from human ACTB
- Description
- Affinity chromatography on Protein A
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Contribution of rearranged actin structures to the spread of Ectromelia virus infection in vitro.
Sex-specific proteome differences in the anterior cingulate cortex of schizophrenia.
Identification of a hormone-regulated dynamic nuclear actin network associated with estrogen receptor alpha in human breast cancer cell nuclei.
Tyrosine phosphorylation of cofilin at Y68 by v-Src leads to its degradation through ubiquitin-proteasome pathway.
Beta and gamma-cytoplasmic actins display distinct distribution and functional diversity.
Boratynska A, Martyniszyn L, Szulc L, Krzyzowska M, Szczepanowska J, Niemialtowski MG
Acta virologica 2010;54(1):41-8
Acta virologica 2010;54(1):41-8
Sex-specific proteome differences in the anterior cingulate cortex of schizophrenia.
Martins-de-Souza D, Schmitt A, Röder R, Lebar M, Schneider-Axmann T, Falkai P, Turck CW
Journal of psychiatric research 2010 Oct;44(14):989-91
Journal of psychiatric research 2010 Oct;44(14):989-91
Identification of a hormone-regulated dynamic nuclear actin network associated with estrogen receptor alpha in human breast cancer cell nuclei.
Ambrosino C, Tarallo R, Bamundo A, Cuomo D, Franci G, Nassa G, Paris O, Ravo M, Giovane A, Zambrano N, Lepikhova T, Jänne OA, Baumann M, Nyman TA, Cicatiello L, Weisz A
Molecular & cellular proteomics : MCP 2010 Jun;9(6):1352-67
Molecular & cellular proteomics : MCP 2010 Jun;9(6):1352-67
Tyrosine phosphorylation of cofilin at Y68 by v-Src leads to its degradation through ubiquitin-proteasome pathway.
Yoo Y, Ho HJ, Wang C, Guan JL
Oncogene 2010 Jan 14;29(2):263-72
Oncogene 2010 Jan 14;29(2):263-72
Beta and gamma-cytoplasmic actins display distinct distribution and functional diversity.
Dugina V, Zwaenepoel I, Gabbiani G, Clément S, Chaponnier C
Journal of cell science 2009 Aug 15;122(Pt 16):2980-8
Journal of cell science 2009 Aug 15;122(Pt 16):2980-8
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of ACTB (arrow) using rabbit polyclonal ACTB Antibody (Cat. #AP50066PU-N). 293 cell lysates (2 μg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ACTB gene.
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- ACTB Antibody (Cat. #AP50066PU-N) western blot analysis in Jurkat cell line lysates (35 μg/lane).This demonstrates the EKI2 antibody detected the EKI2 protein (arrow).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- ACTB Antibody (Cat. #AP50066PU-N) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ACTB Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- ACTB Antibody (Cat. #AP50066PU-N) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.