AM1829b

antibody from Abgent

Targeting: ACTB

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM1829b

Provider

Abgent

Proper citation

Abgent Cat#AM1829b, RRID:AB_10664137

Product name

Beta-Actin Antibody

Antibody type

Monoclonal

Antigen

Recombinant Protein

Description

Mouse Monoclonal Antibody (Mab)

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

References

Submitted references

Evidence for a novel antioxidant function and isoform-specific regulation of the human p66Shc gene.

Miyazawa M, Tsuji Y
Molecular biology of the cell 2014 Jul 1;25(13):2116-27

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Functional analyses of the three simian hemorrhagic fever virus nonstructural protein 1 papain-like proteases.

Vatter HA, Di H, Donaldson EF, Radu GU, Maines TR, Brinton MA
Journal of virology 2014 Aug;88(16):9129-40

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Addiction to multiple oncogenes can be exploited to prevent the emergence of therapeutic resistance.

Choi PS, Li Y, Felsher DW
Proceedings of the National Academy of Sciences of the United States of America 2014 Aug 12;111(32):E3316-24

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Western blot analysis of anti-ACTB Antibody (Cat. #AM1829b) in K562, HL-60,Hela cell line, mouse spleen, mouse liver tissue lysates, mouse NIH-3T3 cell line lysate and mouse cerebellum, mouse brain tissue lysates (35ug/lane). ACTB (arrow) was detected using the purified Mab. "

Primary Ab dilution

1:1000

Immunocytochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of ACTB Antibody (Cat#AM1829b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).

Primary Ab dilution

1:10~50

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

ACTB Antibody (AM1829b)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ACTB Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

Primary Ab dilution

1:10~50