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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Western blot [7]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- GTX100313 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX100313, RRID:AB_11174236
- Product name
- beta Actin antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Synthesis of carbazole derivatives containing chalcone analogs as non-intercalative topoisomerase II catalytic inhibitors and apoptosis inducers.
Bispecific Antibodies and Antibody-Drug Conjugates (ADCs) Bridging HER2 and Prolactin Receptor Improve Efficacy of HER2 ADCs.
Flavanol metabolites reduce monocyte adhesion to endothelial cells through modulation of expression of genes via p38-MAPK and p65-Nf-kB pathways.
Quercetin 3-O-methyl ether protects FL83B cells from copper induced oxidative stress through the PI3K/Akt and MAPK/Erk pathway.
The protective effects of spirulina in cyclophosphamide induced nephrotoxicity and urotoxicity in rats.
Li PH, Jiang H, Zhang WJ, Li YL, Zhao MC, Zhou W, Zhang LY, Tang YD, Dong CZ, Huang ZS, Chen HX, Du ZY
European journal of medicinal chemistry 2018 Feb 10;145:498-510
European journal of medicinal chemistry 2018 Feb 10;145:498-510
Bispecific Antibodies and Antibody-Drug Conjugates (ADCs) Bridging HER2 and Prolactin Receptor Improve Efficacy of HER2 ADCs.
Andreev J, Thambi N, Perez Bay AE, Delfino F, Martin J, Kelly MP, Kirshner JR, Rafique A, Kunz A, Nittoli T, MacDonald D, Daly C, Olson W, Thurston G
Molecular cancer therapeutics 2017 Apr;16(4):681-693
Molecular cancer therapeutics 2017 Apr;16(4):681-693
Flavanol metabolites reduce monocyte adhesion to endothelial cells through modulation of expression of genes via p38-MAPK and p65-Nf-kB pathways.
Claude S, Boby C, Rodriguez-Mateos A, Spencer JP, Gérard N, Morand C, Milenkovic D
Molecular nutrition & food research 2014 May;58(5):1016-27
Molecular nutrition & food research 2014 May;58(5):1016-27
Quercetin 3-O-methyl ether protects FL83B cells from copper induced oxidative stress through the PI3K/Akt and MAPK/Erk pathway.
Tseng HL, Li CJ, Huang LH, Chen CY, Tsai CH, Lin CN, Hsu HY
Toxicology and applied pharmacology 2012 Oct 1;264(1):104-13
Toxicology and applied pharmacology 2012 Oct 1;264(1):104-13
The protective effects of spirulina in cyclophosphamide induced nephrotoxicity and urotoxicity in rats.
Sinanoglu O, Yener AN, Ekici S, Midi A, Aksungar FB
Urology 2012 Dec;80(6):1392.e1-6
Urology 2012 Dec;80(6):1392.e1-6
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (10 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX100313) diluted at 1:5000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE GTX100313 diluted at 1:5000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE Beta-actin antibody GTX100313 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- beta Actin antibody detects beta Actin protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX100313) diluted at a dilution of 1:1000.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 £gg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX100313) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (10 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX100313) diluted at 1:5000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX100313) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using beta Actin(GTX100313) antibody (Green) at 1:1000 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- beta Actin antibody detects beta Actin protein at cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: beta Actin protein stained by beta Actin antibody (GTX100313) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded U373 xenograft, using beta Actin(GTX100313) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- beta Actin antibody detects beta Actin protein at cytosol on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. beta Actin antibody (GTX100313) dilution: 1:500.
