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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AM1021B - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AM1021b, RRID:AB_2257945
- Product name
- Beta-actin Antibody
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Purified Mouse Monoclonal Antibody (Mab)
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Vial size
- 400 µl
- Concentration
- None
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references The Changes of 8-OHdG, hOGG1, APE1 and Pol β in Lenses of Patients with Age-Related Cataract.
Reversal of cocaine-conditioned place preference through methyl supplementation in mice: altering global DNA methylation in the prefrontal cortex.
Insulin-like growth factor binding protein 7, a member of insulin-like growth factor signal pathway, involved in immune response of small abalone Haliotis diversicolor.
Insulin-like growth factor 1 enhances the proliferation and osteogenic differentiation of human periodontal ligament stem cells via ERK and JNK MAPK pathways.
Dentinogenic capacity: immature root papilla stem cells versus mature root pulp stem cells.
Levels of Rabs and WAVE family proteins associated with translocation of GLUT4 to the cell surface in endometria from hyperinsulinemic PCOS women.
Xu B, Kang L, Zhang G, Wu J, Zhu R, Yang M, Guan H
Current eye research 2014 Jun 9;:1-8
Current eye research 2014 Jun 9;:1-8
Reversal of cocaine-conditioned place preference through methyl supplementation in mice: altering global DNA methylation in the prefrontal cortex.
Tian W, Zhao M, Li M, Song T, Zhang M, Quan L, Li S, Sun ZS
PloS one 2012;7(3):e33435
PloS one 2012;7(3):e33435
Insulin-like growth factor binding protein 7, a member of insulin-like growth factor signal pathway, involved in immune response of small abalone Haliotis diversicolor.
Li N, Zhang Z, Zhang L, Wang S, Zou Z, Wang G, Wang Y
Fish & shellfish immunology 2012 Aug;33(2):229-42
Fish & shellfish immunology 2012 Aug;33(2):229-42
Insulin-like growth factor 1 enhances the proliferation and osteogenic differentiation of human periodontal ligament stem cells via ERK and JNK MAPK pathways.
Yu Y, Mu J, Fan Z, Lei G, Yan M, Wang S, Tang C, Wang Z, Yu J, Zhang G
Histochemistry and cell biology 2012 Apr;137(4):513-25
Histochemistry and cell biology 2012 Apr;137(4):513-25
Dentinogenic capacity: immature root papilla stem cells versus mature root pulp stem cells.
Lei G, Yan M, Wang Z, Yu Y, Tang C, Wang Z, Yu J, Zhang G
Biology of the cell / under the auspices of the European Cell Biology Organization 2011 Apr;103(4):185-96
Biology of the cell / under the auspices of the European Cell Biology Organization 2011 Apr;103(4):185-96
Levels of Rabs and WAVE family proteins associated with translocation of GLUT4 to the cell surface in endometria from hyperinsulinemic PCOS women.
Rosas C, Gabler F, Vantman D, Romero C, Vega M
Human reproduction (Oxford, England) 2010 Nov;25(11):2870-7
Human reproduction (Oxford, England) 2010 Nov;25(11):2870-7
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Western blot analysis of lysates from HepG2, HL-60, mouse NIH/3T3 cell line, mouse cerebellum and rat stomach tissue lysate, CHO, COS-7 cell line lysate(from left to right), using Beta-actin Antibody(Cat. #AM1021b). AM1021b was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane."
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Beta-actin Monoclonal Antibody (Cat. #AM1021B) immunohistochemistry analysis in formalin fixed and paraffin embedded human esophageal carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Beta-actin Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
- Primary Ab dilution
- 1:10~50
