Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- OAAB00174 - Provider product page
- Provider
- Aviva Systems Biology
- Product name
- ACTB Antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 400ul
- Storage
- Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- ACTB Antibody western blot analysis in Jurkat cell lysates (35ug/lane).This demonstrates the EKI2 antibody detected the EKI2 protein (arrow).
- Sample type
- Jurkat cell line lysates
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis of ACTB (arrow) using rabbit polyclonal ACTB Antibody either nontransfected (Lane 1) or lysates transiently transfected transfected (Lane 2) with the ACTB gene.
- Sample type
- lysatestransiently transfected with the ACTB gene.
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- ACTB Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ACTB Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
- Sample type
- human tonsil tissue
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol