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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [123]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Flow cytometry [1]
- Other assay [2]
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- Product number
- AM4302 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- beta Actin Monoclonal Antibody (AC-15)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- The anti-beta-actin monoclonal antibody can be used to analyze beta-actin protein expression in experimental cells by immunofluorescence and western blot and has been specifically used in combination with beta-actin siRNA control to study the specificity and effectiveness of the RNA Interference (RNAi) effect at the protein level. The antibody reacts with beta-actin expressing cells in human, bovine, sheep, pig, rabbit, cat, dog, mouse, rat, guinea pig, chicken, carp, leech and fruit fly tissues, but not in amoeba. Use Anti-beta-actin for detection of beta-actin by ELISA, immunohistology, western blotting, and immunofluorescence. The antibody specifically recognizes beta-actin in a wide variety of tissues and species using immunoblotting, immunofluorescent staining of cultured cell lines, and immunohistochemistry. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding. Ethanol, B5, methacarn, or Bouin's solutions may also be used as fixatives.
- Reactivity
- Human, Bovine, Canine, Chicken/Avian, Drosophila, Feline, Guinea Pig, Porcine, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- AC-15
- Vial size
- 100 µg
- Concentration
- Conc. Not Determined
- Storage
- Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Submitted references Mild membrane depolarization in neurons induces immediate early gene transcription and acutely subdues responses to a successive stimulus.
Alpha-fetoprotein can promote gastric cancer progression via upregulation of metastasis-associated colon cancer 1.
The ubiquitin-dependent ATPase p97 removes cytotoxic trapped PARP1 from chromatin.
Functional analysis of the 1p34.3 risk locus implicates GNL2 in high-grade serous ovarian cancer.
Recurrent evolution of an inhibitor of ESCRT-dependent virus budding and LINE-1 retrotransposition in primates.
Knockdown of VDAC1 alleviates the cognitive dysfunction secondary to sepsis-associated encephalopathy.
siRNA Knockdown of REDD1 Facilitates Aspirin-Mediated Dephosphorylation of mTORC1 Target 4E-BP1 in MDA-MB-468 Human Breast Cancer Cell Line.
SARS-CoV-2 Disrupts Proximal Elements in the JAK-STAT Pathway.
Melatonin Reduces Oxidative Stress in the Right Ventricle of Newborn Sheep Gestated under Chronic Hypoxia.
Functional expression of complement factor I following AAV-mediated gene delivery in the retina of mice and human cells.
p97/VCP inhibition causes excessive MRE11-dependent DNA end resection promoting cell killing after ionizing radiation.
Identification of Nucleolin as a Novel AEG-1-Interacting Protein in Breast Cancer via Interactome Profiling.
Glycogen Synthase Kinase 3 Beta Regulates the Human Aryl Hydrocarbon Receptor Cellular Content and Activity.
Germline nuclear-predominant Pten murine model exhibits impaired social and perseverative behavior, microglial activation, and increased oxytocinergic activity.
MAGI-1 PDZ2 Domain Blockade Averts Adenovirus Infection via Enhanced Proteolysis of the Apical Coxsackievirus and Adenovirus Receptor.
CXCR3 alleviates renal ischemia‑reperfusion injury via increase of Tregs.
Up-regulation of gasdermin C in mouse small intestine is associated with lytic cell death in enterocytes in worm-induced type 2 immunity.
LncRNA MNX1-AS1 Contributes to Laryngeal Squamous Cell Carcinoma Growth and Migration by Regulating mir-744-5p/bcl9/β-Catenin Axis.
The Presence of Cholesteryl Ester Transfer Protein (CETP) in Endothelial Cells Generates Vascular Oxidative Stress and Endothelial Dysfunction.
Non-canonical Glutamate-Cysteine Ligase Activity Protects against Ferroptosis.
Pseudomonas aeruginosa survives in epithelia by ExoS-mediated inhibition of autophagy and mTOR.
The protease SPRTN and SUMOylation coordinate DNA-protein crosslink repair to prevent genome instability.
Pulmonary ACE2 expression in neonatal and adult rats.
Low-Density Lipoprotein Receptor Suppresses the Endogenous Cholesterol Synthesis Pathway To Oppose Gammaherpesvirus Replication in Primary Macrophages.
LXH254, a Potent and Selective ARAF-Sparing Inhibitor of BRAF and CRAF for the Treatment of MAPK-Driven Tumors.
PHGDH supports liver ceramide synthesis and sustains lipid homeostasis.
HSPA12B Secreted by Tumor-Associated Endothelial Cells Might Induce M2 Polarization of Macrophages via Activating PI3K/Akt/mTOR Signaling.
Genes Regulated by HPV 16 E6 and High Expression of NFX1-123 in Cervical Cancers.
SARS-CoV-2 desensitizes host cells to interferon through inhibition of the JAK-STAT pathway.
KRAS K104 modification affects the KRAS(G12D)-GEF interaction and mediates cell growth and motility.
MINPP1 prevents intracellular accumulation of the chelator inositol hexakisphosphate and is mutated in Pontocerebellar Hypoplasia.
The Hepatitis B Virus Pre-Core Protein p22 Activates Wnt Signaling.
Certain ortho-hydroxylated brominated ethers are promiscuous kinase inhibitors that impair neuronal signaling and neurodevelopmental processes.
Interplay of ERα binding and DNA methylation in the intron-2 determines the expression and estrogen regulation of cystatin A in breast cancer cells.
Dose and duration of interferon γ pre-licensing interact with donor characteristics to influence the expression and function of indoleamine-2,3-dioxygenase in mesenchymal stromal cells.
Inclusion of PF68 Surfactant Improves Stability of rAAV Titer when Passed through a Surgical Device Used in Retinal Gene Therapy.
Cell-Based Phenotypic Drug Screening Identifies Luteolin as Candidate Therapeutic for Nephropathic Cystinosis.
An improved method for high-throughput quantification of autophagy in mammalian cells.
Dynamic and regulated TAF gene expression during mouse embryonic germ cell development.
Context-dependent activation of SIRT3 is necessary for anchorage-independent survival and metastasis of ovarian cancer cells.
Association of Messenger RNA Level With Phenotype in Patients With Choroideremia: Potential Implications for Gene Therapy Dose.
Generation of genomic-integration-free human induced pluripotent stem cells and the derived cardiomyocytes of X-linked dilated cardiomyopathy from DMD gene mutation.
The protein kinase Akt acts as a coat adaptor in endocytic recycling.
Eugenol attenuates inflammatory response and enhances barrier function during lipopolysaccharide-induced inflammation in the porcine intestinal epithelial cells.
HIV Tat-mediated induction of autophagy regulates the disruption of ZO-1 in brain endothelial cells.
Defective base excision repair in the response to DNA damaging agents in triple negative breast cancer.
Thyronamine regulation of TAAR1 expression in breast cancer cells and investigation of its influence on viability and migration.
ALDH7A1 inhibits the intracellular transport pathways during hypoxia and starvation to promote cellular energy homeostasis.
Cul3 regulates cyclin E1 protein abundance via a degron located within the N-terminal region of cyclin E.
TRAF6 Plays a Proviral Role in Tick-Borne Flavivirus Infection through Interaction with the NS3 Protease.
Determination and analysis of agonist and antagonist potential of naturally occurring flavonoids for estrogen receptor (ERα) by various parameters and molecular modelling approach.
Antioxidant and immunomodulatory activity induced by stevioside in liver damage: In vivo, in vitro and in silico assays.
CoCl(2) simulated hypoxia induce cell proliferation and alter the expression pattern of hypoxia associated genes involved in angiogenesis and apoptosis.
Identification of NFAT5 as a transcriptional regulator of the EDN1 gene in collecting duct.
An extracellular Leptospira interrogans leucine-rich repeat protein binds human E- and VE-cadherins.
Mitochondrial hypoxic stress induces widespread RNA editing by APOBEC3G in natural killer cells.
Hyperosmotic stress induces cell-dependent aggregation of α-synuclein.
4'-Phosphopantetheine corrects CoA, iron, and dopamine metabolic defects in mammalian models of PKAN.
Lack of mitochondrial NADP(H)-transhydrogenase expression in macrophages exacerbates atherosclerosis in hypercholesterolemic mice.
Stevia Prevents Acute and Chronic Liver Injury Induced by Carbon Tetrachloride by Blocking Oxidative Stress through Nrf2 Upregulation.
High N-glycan multiplicity is critical for neuronal adhesion and sensitizes the developing cerebellum to N-glycosylation defect.
Sodium nitrite-derived nitric oxide protects rat testes against ischemia/reperfusion injury.
Downregulation of SNCA Expression by Targeted Editing of DNA Methylation: A Potential Strategy for Precision Therapy in PD.
Nephron prorenin receptor deficiency alters renal medullary endothelin-1 and endothelin receptor expression.
Double-stranded RNA innate immune response activation from long-term adeno-associated virus vector transduction.
The Biological Activity of AAV Vectors for Choroideremia Gene Therapy Can Be Measured by In Vitro Prenylation of RAB6A.
Persistent 6-OH-BDE-47 exposure impairs functional neuronal maturation and alters expression of neurodevelopmentally-relevant chromatin remodelers.
Oligomannosidic glycans at Asn-110 are essential for secretion of human diamine oxidase.
HIV-1 intron-containing RNA expression induces innate immune activation and T cell dysfunction.
Specific Alterations in Astrocyte Properties via the GluA2-GAPDH Complex Associated with Multiple Sclerosis.
Binase treatment increases interferon sensitivity and apoptosis in SiHa cervical carcinoma cells by downregulating E6 and E7 human papilloma virus oncoproteins.
BRAF-inhibitor Associated MEK Mutations Increase RAF-Dependent and -Independent Enzymatic Activity.
Genome-wide identification and differential analysis of translational initiation.
Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor Sequestration in Triple-Negative Breast Cancer.
Transient overexpression of exogenous APOBEC3A causes C-to-U RNA editing of thousands of genes.
Glycation potentiates α-synuclein-associated neurodegeneration in synucleinopathies.
Brilliant cresyl blue staining does not present cytotoxic effects on human luteinized follicular cells, according to gene/protein expression, as well as to cytotoxicity tests.
The mechanism of sirtuin 2-mediated exacerbation of alpha-synuclein toxicity in models of Parkinson disease.
Histone Hypervariants H2A.Z.1 and H2A.Z.2 Play Independent and Context-Specific Roles in Neuronal Activity-Induced Transcription of Arc/Arg3.1 and Other Immediate Early Genes.
Starved epithelial cells uptake extracellular matrix for survival.
Codon-Optimized RPGR Improves Stability and Efficacy of AAV8 Gene Therapy in Two Mouse Models of X-Linked Retinitis Pigmentosa.
Mitochondrial complex II regulates a distinct oxygen sensing mechanism in monocytes.
Glycation potentiates neurodegeneration in models of Huntington's disease.
A Syndromic Intellectual Disability Disorder Caused by Variants in TELO2, a Gene Encoding a Component of the TTT Complex.
Validation data of a rabbit antiserum and affinity purified polyclonal antibody against the N-terminus of human GPR30.
Parallel Regulation of von Hippel-Lindau Disease by pVHL-Mediated Degradation of B-Myb and Hypoxia-Inducible Factor α.
Histone H3.3 promotes IgV gene diversification by enhancing formation of AID-accessible single-stranded DNA.
Bromodomain inhibition of the transcriptional coactivators CBP/EP300 as a therapeutic strategy to target the IRF4 network in multiple myeloma.
The Epigenetic Regulator HDAC1 Modulates Transcription of a Core Cardiogenic Program in Human Cardiac Mesenchymal Stromal Cells Through a p53-Dependent Mechanism.
The double-domain cytidine deaminase APOBEC3G is a cellular site-specific RNA editing enzyme.
High-Order Drug Combinations Are Required to Effectively Kill Colorectal Cancer Cells.
Interleukin-6: A Constitutive Modulator of Glycoprotein 130, Neuroinflammatory and Cell Survival Signaling in Retina.
Transcriptome-wide identification of mRNAs and lincRNAs associated with trastuzumab-resistance in HER2-positive breast cancer.
Improved Method for Isolation of Neonatal Rat Cardiomyocytes with Increased Yield of C-Kit+ Cardiac Progenitor Cells.
Non-Specific Blocking of miR-17-5p Guide Strand in Triple Negative Breast Cancer Cells by Amplifying Passenger Strand Activity.
Impact of Diverse Immune Evasion Mechanisms of Cancer Cells on T Cells Engaged by EpCAM/CD3-Bispecific Antibody Construct AMG 110.
Deregulated Expression of SRC, LYN and CKB Kinases by DNA Methylation and Its Potential Role in Gastric Cancer Invasiveness and Metastasis.
Bacillus anthracis spores germinate extracellularly at air-liquid interface in an in vitro lung model under serum-free conditions.
A sequence-specific DNA binding small molecule triggers the release of immunogenic signals and phagocytosis in a model of B-cell lymphoma.
Elevated α-synuclein caused by SNCA gene triplication impairs neuronal differentiation and maturation in Parkinson's patient-derived induced pluripotent stem cells.
Safety and angiogenic effects of systemic gene delivery of a modified erythropoietin.
Estrogen Replacement Therapy in Ovariectomized Nonpregnant Ewes Stimulates Uterine Artery Hydrogen Sulfide Biosynthesis by Selectively Up-Regulating Cystathionine β-Synthase Expression.
Activation of Adenosine Monophosphate-Activated Protein Kinase Is an Additional Mechanism That Participates in Mediating Inhibitory Actions of Prostaglandin F2Alpha in Mature, but Not Developing, Bovine Corpora Lutea.
The RNA-binding protein LIN28B regulates developmental timing in the mammalian cochlea.
Mutations in NONO lead to syndromic intellectual disability and inhibitory synaptic defects.
An analysis of critical factors for quantitative immunoblotting.
APOBEC3A cytidine deaminase induces RNA editing in monocytes and macrophages.
Serum stability and physicochemical characterization of a novel amphipathic peptide C6M1 for siRNA delivery.
Mesenchymal stem cells contribute to the chemoresistance of hepatocellular carcinoma cells in inflammatory environment by inducing autophagy.
Cyclin C is a haploinsufficient tumour suppressor.
Mechanisms of intracellular calcium homeostasis in developing and mature bovine corpora lutea.
Determinants of Moloney murine leukemia virus Gag-Pol and genomic RNA proportions.
A time- and matrix-dependent TGFBR3-JUND-KRT5 regulatory circuit in single breast epithelial cells and basal-like premalignancies.
A noncatalytic function of the ligation complex during nonhomologous end joining.
The type II cGMP dependent protein kinase regulates GluA1 levels at the plasma membrane of developing cerebellar granule cells.
Tumor suppressor Nf2 limits expansion of the neural progenitor pool by inhibiting Yap/Taz transcriptional coactivators.
FcγR-driven release of IL-6 by macrophages requires NOX2-dependent production of reactive oxygen species.
Spatial regulation of interleukin-6 signaling in response to neurodegenerative stressors in the retina.
Moloney murine leukemia virus genomic RNA packaged in the absence of a full complement of wild type nucleocapsid protein.
Transient aggregation of ubiquitinated proteins is a cytosolic unfolded protein response to inflammation and endoplasmic reticulum stress.
Effect of cellular senescence on the albumin permeability of blood-derived endothelial cells.
Identification of cyclophilin-40-interacting proteins reveals potential cellular function of cyclophilin-40.
Beta-arrestin 2 is required for B1 receptor-dependent post-translational activation of inducible nitric oxide synthase.
Rienecker KDA, Poston RG, Segales JS, Finholm IW, Sono MH, Munteanu SJ, Ghaninejad-Esfahani M, Rejepova A, Tejeda-Garibay S, Wickman K, Marron Fernandez de Velasco E, Thayer SA, Saha RN
The Journal of biological chemistry 2022 Sep;298(9):102278
The Journal of biological chemistry 2022 Sep;298(9):102278
Alpha-fetoprotein can promote gastric cancer progression via upregulation of metastasis-associated colon cancer 1.
Mao X, Wang J, Luo F
Oncology letters 2022 Mar;23(3):84
Oncology letters 2022 Mar;23(3):84
The ubiquitin-dependent ATPase p97 removes cytotoxic trapped PARP1 from chromatin.
Krastev DB, Li S, Sun Y, Wicks AJ, Hoslett G, Weekes D, Badder LM, Knight EG, Marlow R, Pardo MC, Yu L, Talele TT, Bartek J, Choudhary JS, Pommier Y, Pettitt SJ, Tutt ANJ, Ramadan K, Lord CJ
Nature cell biology 2022 Jan;24(1):62-73
Nature cell biology 2022 Jan;24(1):62-73
Functional analysis of the 1p34.3 risk locus implicates GNL2 in high-grade serous ovarian cancer.
Nakamura K, Reid BM, Chen A, Chen Z, Goode EL, Permuth JB, Teer JK, Tyrer J, Yu X, Kanetsky PA, Pharoah PD, Gayther SA, Sellers TA, Lawrenson K, Karreth FA
American journal of human genetics 2022 Jan 6;109(1):116-135
American journal of human genetics 2022 Jan 6;109(1):116-135
Recurrent evolution of an inhibitor of ESCRT-dependent virus budding and LINE-1 retrotransposition in primates.
Rheinemann L, Downhour DM, Davenport KA, McKeown AN, Sundquist WI, Elde NC
Current biology : CB 2022 Apr 11;32(7):1511-1522.e6
Current biology : CB 2022 Apr 11;32(7):1511-1522.e6
Knockdown of VDAC1 alleviates the cognitive dysfunction secondary to sepsis-associated encephalopathy.
Cai M, Du B, Si Y, Miao J, Ge J, Zhang J, Song J, Bao H
American journal of translational research 2021;13(7):7538-7555
American journal of translational research 2021;13(7):7538-7555
siRNA Knockdown of REDD1 Facilitates Aspirin-Mediated Dephosphorylation of mTORC1 Target 4E-BP1 in MDA-MB-468 Human Breast Cancer Cell Line.
Savukaitytė A, Gudoitytė G, Bartnykaitė A, Ugenskienė R, Juozaitytė E
Cancer management and research 2021;13:1123-1133
Cancer management and research 2021;13:1123-1133
SARS-CoV-2 Disrupts Proximal Elements in the JAK-STAT Pathway.
Chen DY, Khan N, Close BJ, Goel RK, Blum B, Tavares AH, Kenney D, Conway HL, Ewoldt JK, Chitalia VC, Crossland NA, Chen CS, Kotton DN, Baker SC, Fuchs SY, Connor JH, Douam F, Emili A, Saeed M
Journal of virology 2021 Sep 9;95(19):e0086221
Journal of virology 2021 Sep 9;95(19):e0086221
Melatonin Reduces Oxidative Stress in the Right Ventricle of Newborn Sheep Gestated under Chronic Hypoxia.
Gonzaléz-Candia A, Arias PV, Aguilar SA, Figueroa EG, Reyes RV, Ebensperger G, Llanos AJ, Herrera EA
Antioxidants (Basel, Switzerland) 2021 Oct 22;10(11)
Antioxidants (Basel, Switzerland) 2021 Oct 22;10(11)
Functional expression of complement factor I following AAV-mediated gene delivery in the retina of mice and human cells.
Dreismann AK, McClements ME, Barnard AR, Orhan E, Hughes JP, Lachmann PJ, MacLaren RE
Gene therapy 2021 May;28(5):265-276
Gene therapy 2021 May;28(5):265-276
p97/VCP inhibition causes excessive MRE11-dependent DNA end resection promoting cell killing after ionizing radiation.
Kilgas S, Singh AN, Paillas S, Then CK, Torrecilla I, Nicholson J, Browning L, Vendrell I, Konietzny R, Kessler BM, Kiltie AE, Ramadan K
Cell reports 2021 May 25;35(8):109153
Cell reports 2021 May 25;35(8):109153
Identification of Nucleolin as a Novel AEG-1-Interacting Protein in Breast Cancer via Interactome Profiling.
Lee SJ, Choi KM, Bang G, Park SG, Kim EB, Choi JW, Chung YH, Kim J, Lee SG, Kim E, Kim JY
Cancers 2021 Jun 7;13(11)
Cancers 2021 Jun 7;13(11)
Glycogen Synthase Kinase 3 Beta Regulates the Human Aryl Hydrocarbon Receptor Cellular Content and Activity.
Yang Y, Chan WK
International journal of molecular sciences 2021 Jun 5;22(11)
International journal of molecular sciences 2021 Jun 5;22(11)
Germline nuclear-predominant Pten murine model exhibits impaired social and perseverative behavior, microglial activation, and increased oxytocinergic activity.
Sarn N, Thacker S, Lee H, Eng C
Molecular autism 2021 Jun 4;12(1):41
Molecular autism 2021 Jun 4;12(1):41
MAGI-1 PDZ2 Domain Blockade Averts Adenovirus Infection via Enhanced Proteolysis of the Apical Coxsackievirus and Adenovirus Receptor.
Alghamri MS, Sharma P, Williamson TL, Readler JM, Yan R, Rider SD Jr, Hostetler HA, Cool DR, Kolawole AO, Excoffon KJDA
Journal of virology 2021 Jun 10;95(13):e0004621
Journal of virology 2021 Jun 10;95(13):e0004621
CXCR3 alleviates renal ischemia‑reperfusion injury via increase of Tregs.
Xian W, Wu J, Li Q, Du X, Wang N, Chen D, Gao W, Cao J
Molecular medicine reports 2021 Jul;24(1)
Molecular medicine reports 2021 Jul;24(1)
Up-regulation of gasdermin C in mouse small intestine is associated with lytic cell death in enterocytes in worm-induced type 2 immunity.
Xi R, Montague J, Lin X, Lu C, Lei W, Tanaka K, Zhang YV, Xu X, Zheng X, Zhou X, Urban JF Jr, Iwatsuki K, Margolskee RF, Matsumoto I, Tizzano M, Li J, Jiang P
Proceedings of the National Academy of Sciences of the United States of America 2021 Jul 27;118(30)
Proceedings of the National Academy of Sciences of the United States of America 2021 Jul 27;118(30)
LncRNA MNX1-AS1 Contributes to Laryngeal Squamous Cell Carcinoma Growth and Migration by Regulating mir-744-5p/bcl9/β-Catenin Axis.
Ma B, Ren G, Xu J, Yin C, Shi Y
Cell transplantation 2021 Jan-Dec;30:9636897211005682
Cell transplantation 2021 Jan-Dec;30:9636897211005682
The Presence of Cholesteryl Ester Transfer Protein (CETP) in Endothelial Cells Generates Vascular Oxidative Stress and Endothelial Dysfunction.
Wanschel ACBA, Guizoni DM, Lorza-Gil E, Salerno AG, Paiva AA, Dorighello GG, Davel AP, Balkan W, Hare JM, Oliveira HCF
Biomolecules 2021 Jan 7;11(1)
Biomolecules 2021 Jan 7;11(1)
Non-canonical Glutamate-Cysteine Ligase Activity Protects against Ferroptosis.
Kang YP, Mockabee-Macias A, Jiang C, Falzone A, Prieto-Farigua N, Stone E, Harris IS, DeNicola GM
Cell metabolism 2021 Jan 5;33(1):174-189.e7
Cell metabolism 2021 Jan 5;33(1):174-189.e7
Pseudomonas aeruginosa survives in epithelia by ExoS-mediated inhibition of autophagy and mTOR.
Rao L, De La Rosa I, Xu Y, Sha Y, Bhattacharya A, Holtzman MJ, Gilbert BE, Eissa NT
EMBO reports 2021 Feb 3;22(2):e50613
EMBO reports 2021 Feb 3;22(2):e50613
The protease SPRTN and SUMOylation coordinate DNA-protein crosslink repair to prevent genome instability.
Ruggiano A, Vaz B, Kilgas S, Popović M, Rodriguez-Berriguete G, Singh AN, Higgins GS, Kiltie AE, Ramadan K
Cell reports 2021 Dec 7;37(10):110080
Cell reports 2021 Dec 7;37(10):110080
Pulmonary ACE2 expression in neonatal and adult rats.
Zhao D, Chen X, Han D, Zhong J, Zhang SE, Yang C
FEBS open bio 2021 Aug;11(8):2266-2272
FEBS open bio 2021 Aug;11(8):2266-2272
Low-Density Lipoprotein Receptor Suppresses the Endogenous Cholesterol Synthesis Pathway To Oppose Gammaherpesvirus Replication in Primary Macrophages.
Aurubin CA, Knaack DA, Sahoo D, Tarakanova VL
Journal of virology 2021 Aug 10;95(17):e0064921
Journal of virology 2021 Aug 10;95(17):e0064921
LXH254, a Potent and Selective ARAF-Sparing Inhibitor of BRAF and CRAF for the Treatment of MAPK-Driven Tumors.
Monaco KA, Delach S, Yuan J, Mishina Y, Fordjour P, Labrot E, McKay D, Guo R, Higgins S, Wang HQ, Liang J, Bui K, Green J, Aspesi P, Ambrose J, Mapa F, Griner L, Jaskelioff M, Fuller J, Crawford K, Pardee G, Widger S, Hammerman PS, Engelman JA, Stuart DD, Cooke VG, Caponigro G
Clinical cancer research : an official journal of the American Association for Cancer Research 2021 Apr 1;27(7):2061-2073
Clinical cancer research : an official journal of the American Association for Cancer Research 2021 Apr 1;27(7):2061-2073
PHGDH supports liver ceramide synthesis and sustains lipid homeostasis.
Kang YP, Falzone A, Liu M, González-Sánchez P, Choi BH, Coloff JL, Saller JJ, Karreth FA, DeNicola GM
Cancer & metabolism 2020;8:6
Cancer & metabolism 2020;8:6
HSPA12B Secreted by Tumor-Associated Endothelial Cells Might Induce M2 Polarization of Macrophages via Activating PI3K/Akt/mTOR Signaling.
Zhou J, Zhang A, Fan L
OncoTargets and therapy 2020;13:9103-9111
OncoTargets and therapy 2020;13:9103-9111
Genes Regulated by HPV 16 E6 and High Expression of NFX1-123 in Cervical Cancers.
Chintala S, Levan J, Robinson K, Quist K, Katzenellenbogen RA
OncoTargets and therapy 2020;13:6143-6156
OncoTargets and therapy 2020;13:6143-6156
SARS-CoV-2 desensitizes host cells to interferon through inhibition of the JAK-STAT pathway.
Chen DY, Khan N, Close BJ, Goel RK, Blum B, Tavares AH, Kenney D, Conway HL, Ewoldt JK, Kapell S, Chitalia VC, Crossland NA, Chen CS, Kotton DN, Baker SC, Connor JH, Douam F, Emili A, Saeed M
bioRxiv : the preprint server for biology 2020 Oct 28;
bioRxiv : the preprint server for biology 2020 Oct 28;
KRAS K104 modification affects the KRAS(G12D)-GEF interaction and mediates cell growth and motility.
Chen CC, Hsu CY, Lin HY, Zeng HQ, Cheng KH, Wu CW, Tsai EM, Hsieh TH
Scientific reports 2020 Oct 15;10(1):17447
Scientific reports 2020 Oct 15;10(1):17447
MINPP1 prevents intracellular accumulation of the chelator inositol hexakisphosphate and is mutated in Pontocerebellar Hypoplasia.
Ucuncu E, Rajamani K, Wilson MSC, Medina-Cano D, Altin N, David P, Barcia G, Lefort N, Banal C, Vasilache-Dangles MT, Pitelet G, Lorino E, Rabasse N, Bieth E, Zaki MS, Topcu M, Sonmez FM, Musaev D, Stanley V, Bole-Feysot C, Nitschké P, Munnich A, Bahi-Buisson N, Fossoud C, Giuliano F, Colleaux L, Burglen L, Gleeson JG, Boddaert N, Saiardi A, Cantagrel V
Nature communications 2020 Nov 30;11(1):6087
Nature communications 2020 Nov 30;11(1):6087
The Hepatitis B Virus Pre-Core Protein p22 Activates Wnt Signaling.
Tran BM, Flanagan DJ, Ebert G, Warner N, Tran H, Fifis T, Kastrappis G, Christophi C, Pellegrini M, Torresi J, Phesse TJ, Vincan E
Cancers 2020 May 31;12(6)
Cancers 2020 May 31;12(6)
Certain ortho-hydroxylated brominated ethers are promiscuous kinase inhibitors that impair neuronal signaling and neurodevelopmental processes.
Poston RG, Murphy L, Rejepova A, Ghaninejad-Esfahani M, Segales J, Mulligan K, Saha RN
The Journal of biological chemistry 2020 May 1;295(18):6120-6137
The Journal of biological chemistry 2020 May 1;295(18):6120-6137
Interplay of ERα binding and DNA methylation in the intron-2 determines the expression and estrogen regulation of cystatin A in breast cancer cells.
John Mary DJS, Sikarwar G, Kumar A, Limaye AM
Molecular and cellular endocrinology 2020 Mar 15;504:110701
Molecular and cellular endocrinology 2020 Mar 15;504:110701
Dose and duration of interferon γ pre-licensing interact with donor characteristics to influence the expression and function of indoleamine-2,3-dioxygenase in mesenchymal stromal cells.
Boyt DT, Boland LK, Burand AJ Jr, Brown AJ, Ankrum JA
Journal of the Royal Society, Interface 2020 Jun;17(167):20190815
Journal of the Royal Society, Interface 2020 Jun;17(167):20190815
Inclusion of PF68 Surfactant Improves Stability of rAAV Titer when Passed through a Surgical Device Used in Retinal Gene Therapy.
Patrício MI, Cox CI, Blue C, Barnard AR, Martinez-Fernandez de la Camara C, MacLaren RE
Molecular therapy. Methods & clinical development 2020 Jun 12;17:99-106
Molecular therapy. Methods & clinical development 2020 Jun 12;17:99-106
Cell-Based Phenotypic Drug Screening Identifies Luteolin as Candidate Therapeutic for Nephropathic Cystinosis.
De Leo E, Elmonem MA, Berlingerio SP, Berquez M, Festa BP, Raso R, Bellomo F, Starborg T, Janssen MJ, Abbaszadeh Z, Cairoli S, Goffredo BM, Masereeuw R, Devuyst O, Lowe M, Levtchenko E, Luciani A, Emma F, Rega LR
Journal of the American Society of Nephrology : JASN 2020 Jul;31(7):1522-1537
Journal of the American Society of Nephrology : JASN 2020 Jul;31(7):1522-1537
An improved method for high-throughput quantification of autophagy in mammalian cells.
Koepke L, Winter B, Grenzner A, Regensburger K, Engelhart S, van der Merwe JA, Krebs S, Blum H, Kirchhoff F, Sparrer KMJ
Scientific reports 2020 Jul 22;10(1):12241
Scientific reports 2020 Jul 22;10(1):12241
Dynamic and regulated TAF gene expression during mouse embryonic germ cell development.
Gura MA, Mikedis MM, Seymour KA, de Rooij DG, Page DC, Freiman RN
PLoS genetics 2020 Jan;16(1):e1008515
PLoS genetics 2020 Jan;16(1):e1008515
Context-dependent activation of SIRT3 is necessary for anchorage-independent survival and metastasis of ovarian cancer cells.
Kim YS, Gupta Vallur P, Jones VM, Worley BL, Shimko S, Shin DH, Crawford LC, Chen CW, Aird KM, Abraham T, Shepherd TG, Warrick JI, Lee NY, Phaeton R, Mythreye K, Hempel N
Oncogene 2020 Feb;39(8):1619-1633
Oncogene 2020 Feb;39(8):1619-1633
Association of Messenger RNA Level With Phenotype in Patients With Choroideremia: Potential Implications for Gene Therapy Dose.
Fry LE, Patrício MI, Williams J, Aylward JW, Hewitt H, Clouston P, Xue K, Barnard AR, MacLaren RE
JAMA ophthalmology 2020 Feb 1;138(2):128-135
JAMA ophthalmology 2020 Feb 1;138(2):128-135
Generation of genomic-integration-free human induced pluripotent stem cells and the derived cardiomyocytes of X-linked dilated cardiomyopathy from DMD gene mutation.
Zhu S, Law AHY, Deng R, Poon ENY, Lo CW, Kwong AKY, Liang R, Chan KYK, Wong WL, Tan-Un KC, Pijnappel WWMP, Chan GCF, Chan SHS
Stem cell research 2020 Dec;49:102040
Stem cell research 2020 Dec;49:102040
The protein kinase Akt acts as a coat adaptor in endocytic recycling.
Hsu JW, Bai M, Li K, Yang JS, Chu N, Cole PA, Eck MJ, Li J, Hsu VW
Nature cell biology 2020 Aug;22(8):927-933
Nature cell biology 2020 Aug;22(8):927-933
Eugenol attenuates inflammatory response and enhances barrier function during lipopolysaccharide-induced inflammation in the porcine intestinal epithelial cells.
Hui Q, Ammeter E, Liu S, Yang R, Lu P, Lahaye L, Yang C
Journal of animal science 2020 Aug 1;98(8)
Journal of animal science 2020 Aug 1;98(8)
HIV Tat-mediated induction of autophagy regulates the disruption of ZO-1 in brain endothelial cells.
Liao K, Niu F, Hu G, Guo ML, Sil S, Buch S
Tissue barriers 2020 Apr 2;8(2):1748983
Tissue barriers 2020 Apr 2;8(2):1748983
Defective base excision repair in the response to DNA damaging agents in triple negative breast cancer.
Lee KJ, Piett CG, Andrews JF, Mann E, Nagel ZD, Gassman NR
PloS one 2019;14(10):e0223725
PloS one 2019;14(10):e0223725
Thyronamine regulation of TAAR1 expression in breast cancer cells and investigation of its influence on viability and migration.
Tremmel E, Hofmann S, Kuhn C, Heidegger H, Heublein S, Hermelink K, Wuerstlein R, Harbeck N, Mayr D, Mahner S, Ditsch N, Jeschke U, Vattai A
Breast cancer (Dove Medical Press) 2019;11:87-97
Breast cancer (Dove Medical Press) 2019;11:87-97
ALDH7A1 inhibits the intracellular transport pathways during hypoxia and starvation to promote cellular energy homeostasis.
Yang JS, Hsu JW, Park SY, Lee SY, Li J, Bai M, Alves C, Tseng W, Michelet X, Ho IC, Hsu VW
Nature communications 2019 Sep 6;10(1):4068
Nature communications 2019 Sep 6;10(1):4068
Cul3 regulates cyclin E1 protein abundance via a degron located within the N-terminal region of cyclin E.
Davidge B, Rebola KGO, Agbor LN, Sigmund CD, Singer JD
Journal of cell science 2019 Nov 6;132(21)
Journal of cell science 2019 Nov 6;132(21)
TRAF6 Plays a Proviral Role in Tick-Borne Flavivirus Infection through Interaction with the NS3 Protease.
Youseff BH, Brewer TG, McNally KL, Izuogu AO, Lubick KJ, Presloid JB, Alqahtani S, Chattopadhyay S, Best SM, Hu X, Taylor RT
iScience 2019 May 31;15:489-501
iScience 2019 May 31;15:489-501
Determination and analysis of agonist and antagonist potential of naturally occurring flavonoids for estrogen receptor (ERα) by various parameters and molecular modelling approach.
Puranik NV, Srivastava P, Bhatt G, John Mary DJS, Limaye AM, Sivaraman J
Scientific reports 2019 May 15;9(1):7450
Scientific reports 2019 May 15;9(1):7450
Antioxidant and immunomodulatory activity induced by stevioside in liver damage: In vivo, in vitro and in silico assays.
Casas-Grajales S, Ramos-Tovar E, Chávez-Estrada E, Alvarez-Suarez D, Hernández-Aquino E, Reyes-Gordillo K, Cerda-García-Rojas CM, Camacho J, Tsutsumi V, Lakshman MR, Muriel P
Life sciences 2019 May 1;224:187-196
Life sciences 2019 May 1;224:187-196
CoCl(2) simulated hypoxia induce cell proliferation and alter the expression pattern of hypoxia associated genes involved in angiogenesis and apoptosis.
Rana NK, Singh P, Koch B
Biological research 2019 Mar 15;52(1):12
Biological research 2019 Mar 15;52(1):12
Identification of NFAT5 as a transcriptional regulator of the EDN1 gene in collecting duct.
Lakshmipathi J, Wheatley W, Kumar A, Mercenne G, Rodan AR, Kohan DE
American journal of physiology. Renal physiology 2019 Mar 1;316(3):F481-F487
American journal of physiology. Renal physiology 2019 Mar 1;316(3):F481-F487
An extracellular Leptospira interrogans leucine-rich repeat protein binds human E- and VE-cadherins.
Eshghi A, Gaultney RA, England P, Brûlé S, Miras I, Sato H, Coburn J, Bellalou J, Moriarty TJ, Haouz A, Picardeau M
Cellular microbiology 2019 Feb;21(2):e12949
Cellular microbiology 2019 Feb;21(2):e12949
Mitochondrial hypoxic stress induces widespread RNA editing by APOBEC3G in natural killer cells.
Sharma S, Wang J, Alqassim E, Portwood S, Cortes Gomez E, Maguire O, Basse PH, Wang ES, Segal BH, Baysal BE
Genome biology 2019 Feb 21;20(1):37
Genome biology 2019 Feb 21;20(1):37
Hyperosmotic stress induces cell-dependent aggregation of α-synuclein.
Fragniere AMC, Stott SRW, Fazal SV, Andreasen M, Scott K, Barker RA
Scientific reports 2019 Feb 19;9(1):2288
Scientific reports 2019 Feb 19;9(1):2288
4'-Phosphopantetheine corrects CoA, iron, and dopamine metabolic defects in mammalian models of PKAN.
Jeong SY, Hogarth P, Placzek A, Gregory AM, Fox R, Zhen D, Hamada J, van der Zwaag M, Lambrechts R, Jin H, Nilsen A, Cobb J, Pham T, Gray N, Ralle M, Duffy M, Schwanemann L, Rai P, Freed A, Wakeman K, Woltjer RL, Sibon OC, Hayflick SJ
EMBO molecular medicine 2019 Dec;11(12):e10489
EMBO molecular medicine 2019 Dec;11(12):e10489
Lack of mitochondrial NADP(H)-transhydrogenase expression in macrophages exacerbates atherosclerosis in hypercholesterolemic mice.
Salerno AG, Rentz T, Dorighello GG, Marques AC, Lorza-Gil E, Wanschel ACBA, de Moraes A, Vercesi AE, Oliveira HCF
The Biochemical journal 2019 Dec 23;476(24):3769-3789
The Biochemical journal 2019 Dec 23;476(24):3769-3789
Stevia Prevents Acute and Chronic Liver Injury Induced by Carbon Tetrachloride by Blocking Oxidative Stress through Nrf2 Upregulation.
Ramos-Tovar E, Hernández-Aquino E, Casas-Grajales S, Buendia-Montaño LD, Galindo-Gómez S, Camacho J, Tsutsumi V, Muriel P
Oxidative medicine and cellular longevity 2018;2018:3823426
Oxidative medicine and cellular longevity 2018;2018:3823426
High N-glycan multiplicity is critical for neuronal adhesion and sensitizes the developing cerebellum to N-glycosylation defect.
Medina-Cano D, Ucuncu E, Nguyen LS, Nicouleau M, Lipecka J, Bizot JC, Thiel C, Foulquier F, Lefort N, Faivre-Sarrailh C, Colleaux L, Guerrera IC, Cantagrel V
eLife 2018 Oct 12;7
eLife 2018 Oct 12;7
Sodium nitrite-derived nitric oxide protects rat testes against ischemia/reperfusion injury.
Lee JW, Lee DH, Park JK, Han JS
Asian journal of andrology 2018 Oct 12;21(1):92-7
Asian journal of andrology 2018 Oct 12;21(1):92-7
Downregulation of SNCA Expression by Targeted Editing of DNA Methylation: A Potential Strategy for Precision Therapy in PD.
Kantor B, Tagliafierro L, Gu J, Zamora ME, Ilich E, Grenier C, Huang ZY, Murphy S, Chiba-Falek O
Molecular therapy : the journal of the American Society of Gene Therapy 2018 Nov 7;26(11):2638-2649
Molecular therapy : the journal of the American Society of Gene Therapy 2018 Nov 7;26(11):2638-2649
Nephron prorenin receptor deficiency alters renal medullary endothelin-1 and endothelin receptor expression.
Ramkumar N, Stuart D, Abraham N, Kohan DE
Physiological research 2018 Jun 27;67(Suppl 1):S127-S136
Physiological research 2018 Jun 27;67(Suppl 1):S127-S136
Double-stranded RNA innate immune response activation from long-term adeno-associated virus vector transduction.
Shao W, Earley LF, Chai Z, Chen X, Sun J, He T, Deng M, Hirsch ML, Ting J, Samulski RJ, Li C
JCI insight 2018 Jun 21;3(12)
JCI insight 2018 Jun 21;3(12)
The Biological Activity of AAV Vectors for Choroideremia Gene Therapy Can Be Measured by In Vitro Prenylation of RAB6A.
Patrício MI, Barnard AR, Cox CI, Blue C, MacLaren RE
Molecular therapy. Methods & clinical development 2018 Jun 15;9:288-295
Molecular therapy. Methods & clinical development 2018 Jun 15;9:288-295
Persistent 6-OH-BDE-47 exposure impairs functional neuronal maturation and alters expression of neurodevelopmentally-relevant chromatin remodelers.
Poston RG, Dunn CJ, Sarkar P, Saha RN
Environmental epigenetics 2018 Jan;4(1):dvx020
Environmental epigenetics 2018 Jan;4(1):dvx020
Oligomannosidic glycans at Asn-110 are essential for secretion of human diamine oxidase.
Gludovacz E, Maresch D, Lopes de Carvalho L, Puxbaum V, Baier LJ, Sützl L, Guédez G, Grünwald-Gruber C, Ulm B, Pils S, Ristl R, Altmann F, Jilma B, Salminen TA, Borth N, Boehm T
The Journal of biological chemistry 2018 Jan 19;293(3):1070-1087
The Journal of biological chemistry 2018 Jan 19;293(3):1070-1087
HIV-1 intron-containing RNA expression induces innate immune activation and T cell dysfunction.
Akiyama H, Miller CM, Ettinger CR, Belkina AC, Snyder-Cappione JE, Gummuluru S
Nature communications 2018 Aug 27;9(1):3450
Nature communications 2018 Aug 27;9(1):3450
Specific Alterations in Astrocyte Properties via the GluA2-GAPDH Complex Associated with Multiple Sclerosis.
Lee FHF, Zhang H, Jiang A, Zai CC, Liu F
Scientific reports 2018 Aug 27;8(1):12856
Scientific reports 2018 Aug 27;8(1):12856
Binase treatment increases interferon sensitivity and apoptosis in SiHa cervical carcinoma cells by downregulating E6 and E7 human papilloma virus oncoproteins.
Mitkevich VA, Burnysheva KM, Petrushanko IY, Adzhubei AA, Schulga AA, Chumakov PM, Makarov AA
Oncotarget 2017 Sep 22;8(42):72666-72675
Oncotarget 2017 Sep 22;8(42):72666-72675
BRAF-inhibitor Associated MEK Mutations Increase RAF-Dependent and -Independent Enzymatic Activity.
Emery CM, Monaco KA, Wang P, Balak M, Freeman A, Meltzer J, Delach SM, Rakiec D, Ruddy DA, Korn JM, Haling J, Acker MG, Caponigro G
Molecular cancer research : MCR 2017 Oct;15(10):1431-1444
Molecular cancer research : MCR 2017 Oct;15(10):1431-1444
Genome-wide identification and differential analysis of translational initiation.
Zhang P, He D, Xu Y, Hou J, Pan BF, Wang Y, Liu T, Davis CM, Ehli EA, Tan L, Zhou F, Hu J, Yu Y, Chen X, Nguyen TM, Rosen JM, Hawke DH, Ji Z, Chen Y
Nature communications 2017 Nov 23;8(1):1749
Nature communications 2017 Nov 23;8(1):1749
Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor Sequestration in Triple-Negative Breast Cancer.
Bajikar SS, Wang CC, Borten MA, Pereira EJ, Atkins KA, Janes KA
Developmental cell 2017 Nov 20;43(4):418-435.e13
Developmental cell 2017 Nov 20;43(4):418-435.e13
Transient overexpression of exogenous APOBEC3A causes C-to-U RNA editing of thousands of genes.
Sharma S, Patnaik SK, Kemer Z, Baysal BE
RNA biology 2017 May 4;14(5):603-610
RNA biology 2017 May 4;14(5):603-610
Glycation potentiates α-synuclein-associated neurodegeneration in synucleinopathies.
Vicente Miranda H, Szego ÉM, Oliveira LMA, Breda C, Darendelioglu E, de Oliveira RM, Ferreira DG, Gomes MA, Rott R, Oliveira M, Munari F, Enguita FJ, Simões T, Rodrigues EF, Heinrich M, Martins IC, Zamolo I, Riess O, Cordeiro C, Ponces-Freire A, Lashuel HA, Santos NC, Lopes LV, Xiang W, Jovin TM, Penque D, Engelender S, Zweckstetter M, Klucken J, Giorgini F, Quintas A, Outeiro TF
Brain : a journal of neurology 2017 May 1;140(5):1399-1419
Brain : a journal of neurology 2017 May 1;140(5):1399-1419
Brilliant cresyl blue staining does not present cytotoxic effects on human luteinized follicular cells, according to gene/protein expression, as well as to cytotoxicity tests.
Alcoba DD, Schneider J, Arruda L, Martiny PB, Capp E, von Eye Corleta H, Brum IS
Reproductive biology 2017 Mar;17(1):60-68
Reproductive biology 2017 Mar;17(1):60-68
The mechanism of sirtuin 2-mediated exacerbation of alpha-synuclein toxicity in models of Parkinson disease.
de Oliveira RM, Vicente Miranda H, Francelle L, Pinho R, Szegö ÉM, Martinho R, Munari F, Lázaro DF, Moniot S, Guerreiro P, Fonseca-Ornelas L, Marijanovic Z, Antas P, Gerhardt E, Enguita FJ, Fauvet B, Penque D, Pais TF, Tong Q, Becker S, Kügler S, Lashuel HA, Steegborn C, Zweckstetter M, Outeiro TF
PLoS biology 2017 Mar;15(3):e2000374
PLoS biology 2017 Mar;15(3):e2000374
Histone Hypervariants H2A.Z.1 and H2A.Z.2 Play Independent and Context-Specific Roles in Neuronal Activity-Induced Transcription of Arc/Arg3.1 and Other Immediate Early Genes.
Dunn CJ, Sarkar P, Bailey ER, Farris S, Zhao M, Ward JM, Dudek SM, Saha RN
eNeuro 2017 Jul-Aug;4(4)
eNeuro 2017 Jul-Aug;4(4)
Starved epithelial cells uptake extracellular matrix for survival.
Muranen T, Iwanicki MP, Curry NL, Hwang J, DuBois CD, Coloff JL, Hitchcock DS, Clish CB, Brugge JS, Kalaany NY
Nature communications 2017 Jan 10;8:13989
Nature communications 2017 Jan 10;8:13989
Codon-Optimized RPGR Improves Stability and Efficacy of AAV8 Gene Therapy in Two Mouse Models of X-Linked Retinitis Pigmentosa.
Fischer MD, McClements ME, Martinez-Fernandez de la Camara C, Bellingrath JS, Dauletbekov D, Ramsden SC, Hickey DG, Barnard AR, MacLaren RE
Molecular therapy : the journal of the American Society of Gene Therapy 2017 Aug 2;25(8):1854-1865
Molecular therapy : the journal of the American Society of Gene Therapy 2017 Aug 2;25(8):1854-1865
Mitochondrial complex II regulates a distinct oxygen sensing mechanism in monocytes.
Sharma S, Wang J, Cortes Gomez E, Taggart RT, Baysal BE
Human molecular genetics 2017 Apr 1;26(7):1328-1339
Human molecular genetics 2017 Apr 1;26(7):1328-1339
Glycation potentiates neurodegeneration in models of Huntington's disease.
Vicente Miranda H, Gomes MA, Branco-Santos J, Breda C, Lázaro DF, Lopes LV, Herrera F, Giorgini F, Outeiro TF
Scientific reports 2016 Nov 18;6:36798
Scientific reports 2016 Nov 18;6:36798
A Syndromic Intellectual Disability Disorder Caused by Variants in TELO2, a Gene Encoding a Component of the TTT Complex.
You J, Sobreira NL, Gable DL, Jurgens J, Grange DK, Belnap N, Siniard A, Szelinger S, Schrauwen I, Richholt RF, Vallee SE, Dinulos MBP, Valle D, Armanios M, Hoover-Fong J
American journal of human genetics 2016 May 5;98(5):909-918
American journal of human genetics 2016 May 5;98(5):909-918
Validation data of a rabbit antiserum and affinity purified polyclonal antibody against the N-terminus of human GPR30.
Manjegowda MC, Gupta PS, Limaye AM
Data in brief 2016 Jun;7:1015-20
Data in brief 2016 Jun;7:1015-20
Parallel Regulation of von Hippel-Lindau Disease by pVHL-Mediated Degradation of B-Myb and Hypoxia-Inducible Factor α.
Okumura F, Uematsu K, Byrne SD, Hirano M, Joo-Okumura A, Nishikimi A, Shuin T, Fukui Y, Nakatsukasa K, Kamura T
Molecular and cellular biology 2016 Jun 15;36(12):1803-17
Molecular and cellular biology 2016 Jun 15;36(12):1803-17
Histone H3.3 promotes IgV gene diversification by enhancing formation of AID-accessible single-stranded DNA.
Romanello M, Schiavone D, Frey A, Sale JE
The EMBO journal 2016 Jul 1;35(13):1452-64
The EMBO journal 2016 Jul 1;35(13):1452-64
Bromodomain inhibition of the transcriptional coactivators CBP/EP300 as a therapeutic strategy to target the IRF4 network in multiple myeloma.
Conery AR, Centore RC, Neiss A, Keller PJ, Joshi S, Spillane KL, Sandy P, Hatton C, Pardo E, Zawadzke L, Bommi-Reddy A, Gascoigne KE, Bryant BM, Mertz JA, Sims RJ
eLife 2016 Jan 5;5
eLife 2016 Jan 5;5
The Epigenetic Regulator HDAC1 Modulates Transcription of a Core Cardiogenic Program in Human Cardiac Mesenchymal Stromal Cells Through a p53-Dependent Mechanism.
Moore JB 4th, Zhao J, Keith MC, Amraotkar AR, Wysoczynski M, Hong KU, Bolli R
Stem cells (Dayton, Ohio) 2016 Dec;34(12):2916-2929
Stem cells (Dayton, Ohio) 2016 Dec;34(12):2916-2929
The double-domain cytidine deaminase APOBEC3G is a cellular site-specific RNA editing enzyme.
Sharma S, Patnaik SK, Taggart RT, Baysal BE
Scientific reports 2016 Dec 15;6:39100
Scientific reports 2016 Dec 15;6:39100
High-Order Drug Combinations Are Required to Effectively Kill Colorectal Cancer Cells.
Horn T, Ferretti S, Ebel N, Tam A, Ho S, Harbinski F, Farsidjani A, Zubrowski M, Sellers WR, Schlegel R, Porter D, Morris E, Wuerthner J, Jeay S, Greshock J, Halilovic E, Garraway LA, Caponigro G, Lehár J
Cancer research 2016 Dec 1;76(23):6950-6963
Cancer research 2016 Dec 1;76(23):6950-6963
Interleukin-6: A Constitutive Modulator of Glycoprotein 130, Neuroinflammatory and Cell Survival Signaling in Retina.
Echevarria FD, Rickman AE, Sappington RM
Journal of clinical & cellular immunology 2016 Aug;7(4)
Journal of clinical & cellular immunology 2016 Aug;7(4)
Transcriptome-wide identification of mRNAs and lincRNAs associated with trastuzumab-resistance in HER2-positive breast cancer.
Merry CR, McMahon S, Forrest ME, Bartels CF, Saiakhova A, Bartel CA, Scacheri PC, Thompson CL, Jackson MW, Harris LN, Khalil AM
Oncotarget 2016 Aug 16;7(33):53230-53244
Oncotarget 2016 Aug 16;7(33):53230-53244
Improved Method for Isolation of Neonatal Rat Cardiomyocytes with Increased Yield of C-Kit+ Cardiac Progenitor Cells.
Rutering J, Ilmer M, Recio A, Coleman M, Vykoukal J, Alt E
Journal of stem cell research & therapy 2015;5(6):1-8
Journal of stem cell research & therapy 2015;5(6):1-8
Non-Specific Blocking of miR-17-5p Guide Strand in Triple Negative Breast Cancer Cells by Amplifying Passenger Strand Activity.
Jin YY, Andrade J, Wickstrom E
PloS one 2015;10(12):e0142574
PloS one 2015;10(12):e0142574
Impact of Diverse Immune Evasion Mechanisms of Cancer Cells on T Cells Engaged by EpCAM/CD3-Bispecific Antibody Construct AMG 110.
Deisting W, Raum T, Kufer P, Baeuerle PA, Münz M
PloS one 2015;10(10):e0141669
PloS one 2015;10(10):e0141669
Deregulated Expression of SRC, LYN and CKB Kinases by DNA Methylation and Its Potential Role in Gastric Cancer Invasiveness and Metastasis.
Mello AA, Leal MF, Rey JA, Pinto GR, Lamarão LM, Montenegro RC, Alves AP, Assumpção PP, Borges Bdo N, Smith MC, Burbano RR
PloS one 2015;10(10):e0140492
PloS one 2015;10(10):e0140492
Bacillus anthracis spores germinate extracellularly at air-liquid interface in an in vitro lung model under serum-free conditions.
Powell JD, Hutchison JR, Hess BM, Straub TM
Journal of applied microbiology 2015 Sep;119(3):711-23
Journal of applied microbiology 2015 Sep;119(3):711-23
A sequence-specific DNA binding small molecule triggers the release of immunogenic signals and phagocytosis in a model of B-cell lymphoma.
Kang JS, Dervan PB
Quarterly reviews of biophysics 2015 Nov;48(4):453-64
Quarterly reviews of biophysics 2015 Nov;48(4):453-64
Elevated α-synuclein caused by SNCA gene triplication impairs neuronal differentiation and maturation in Parkinson's patient-derived induced pluripotent stem cells.
Oliveira LM, Falomir-Lockhart LJ, Botelho MG, Lin KH, Wales P, Koch JC, Gerhardt E, Taschenberger H, Outeiro TF, Lingor P, Schüle B, Arndt-Jovin DJ, Jovin TM
Cell death & disease 2015 Nov 26;6(11):e1994
Cell death & disease 2015 Nov 26;6(11):e1994
Safety and angiogenic effects of systemic gene delivery of a modified erythropoietin.
de Lucas Cerrillo AM, Bond WS, Rex TS
Gene therapy 2015 May;22(5):365-73
Gene therapy 2015 May;22(5):365-73
Estrogen Replacement Therapy in Ovariectomized Nonpregnant Ewes Stimulates Uterine Artery Hydrogen Sulfide Biosynthesis by Selectively Up-Regulating Cystathionine β-Synthase Expression.
Lechuga TJ, Zhang HH, Sheibani L, Karim M, Jia J, Magness RR, Rosenfeld CR, Chen DB
Endocrinology 2015 Jun;156(6):2288-98
Endocrinology 2015 Jun;156(6):2288-98
Activation of Adenosine Monophosphate-Activated Protein Kinase Is an Additional Mechanism That Participates in Mediating Inhibitory Actions of Prostaglandin F2Alpha in Mature, but Not Developing, Bovine Corpora Lutea.
Bowdridge EC, Goravanahally MP, Inskeep EK, Flores JA
Biology of reproduction 2015 Jul;93(1):7
Biology of reproduction 2015 Jul;93(1):7
The RNA-binding protein LIN28B regulates developmental timing in the mammalian cochlea.
Golden EJ, Benito-Gonzalez A, Doetzlhofer A
Proceedings of the National Academy of Sciences of the United States of America 2015 Jul 21;112(29):E3864-73
Proceedings of the National Academy of Sciences of the United States of America 2015 Jul 21;112(29):E3864-73
Mutations in NONO lead to syndromic intellectual disability and inhibitory synaptic defects.
Mircsof D, Langouët M, Rio M, Moutton S, Siquier-Pernet K, Bole-Feysot C, Cagnard N, Nitschke P, Gaspar L, Žnidarič M, Alibeu O, Fritz AK, Wolfer DP, Schröter A, Bosshard G, Rudin M, Koester C, Crestani F, Seebeck P, Boddaert N, Prescott K, DDD Study, Hines R, Moss SJ, Fritschy JM, Munnich A, Amiel J, Brown SA, Tyagarajan SK, Colleaux L
Nature neuroscience 2015 Dec;18(12):1731-6
Nature neuroscience 2015 Dec;18(12):1731-6
An analysis of critical factors for quantitative immunoblotting.
Janes KA
Science signaling 2015 Apr 7;8(371):rs2
Science signaling 2015 Apr 7;8(371):rs2
APOBEC3A cytidine deaminase induces RNA editing in monocytes and macrophages.
Sharma S, Patnaik SK, Taggart RT, Kannisto ED, Enriquez SM, Gollnick P, Baysal BE
Nature communications 2015 Apr 21;6:6881
Nature communications 2015 Apr 21;6:6881
Serum stability and physicochemical characterization of a novel amphipathic peptide C6M1 for siRNA delivery.
Jafari M, Xu W, Pan R, Sweeting CM, Karunaratne DN, Chen P
PloS one 2014;9(5):e97797
PloS one 2014;9(5):e97797
Mesenchymal stem cells contribute to the chemoresistance of hepatocellular carcinoma cells in inflammatory environment by inducing autophagy.
Han Z, Jing Y, Xia Y, Zhang S, Hou J, Meng Y, Yu F, Liu X, Wu M, Zhang P, Wei L
Cell & bioscience 2014;4:22
Cell & bioscience 2014;4:22
Cyclin C is a haploinsufficient tumour suppressor.
Li N, Fassl A, Chick J, Inuzuka H, Li X, Mansour MR, Liu L, Wang H, King B, Shaik S, Gutierrez A, Ordureau A, Otto T, Kreslavsky T, Baitsch L, Bury L, Meyer CA, Ke N, Mulry KA, Kluk MJ, Roy M, Kim S, Zhang X, Geng Y, Zagozdzon A, Jenkinson S, Gale RE, Linch DC, Zhao JJ, Mullighan CG, Harper JW, Aster JC, Aifantis I, von Boehmer H, Gygi SP, Wei W, Look AT, Sicinski P
Nature cell biology 2014 Nov;16(11):1080-91
Nature cell biology 2014 Nov;16(11):1080-91
Mechanisms of intracellular calcium homeostasis in developing and mature bovine corpora lutea.
Wright MF, Bowdridge E, McDermott EL, Richardson S, Scheidler J, Syed Q, Bush T, Inskeep EK, Flores JA
Biology of reproduction 2014 Mar;90(3):55
Biology of reproduction 2014 Mar;90(3):55
Determinants of Moloney murine leukemia virus Gag-Pol and genomic RNA proportions.
Johnson SF, Collins JT, D'Souza VM, Telesnitsky A
Journal of virology 2014 Jul;88(13):7267-75
Journal of virology 2014 Jul;88(13):7267-75
A time- and matrix-dependent TGFBR3-JUND-KRT5 regulatory circuit in single breast epithelial cells and basal-like premalignancies.
Wang CC, Bajikar SS, Jamal L, Atkins KA, Janes KA
Nature cell biology 2014 Apr;16(4):345-56
Nature cell biology 2014 Apr;16(4):345-56
A noncatalytic function of the ligation complex during nonhomologous end joining.
Cottarel J, Frit P, Bombarde O, Salles B, Négrel A, Bernard S, Jeggo PA, Lieber MR, Modesti M, Calsou P
The Journal of cell biology 2013 Jan 21;200(2):173-86
The Journal of cell biology 2013 Jan 21;200(2):173-86
The type II cGMP dependent protein kinase regulates GluA1 levels at the plasma membrane of developing cerebellar granule cells.
Incontro S, Ciruela F, Ziff E, Hofmann F, Sánchez-Prieto J, Torres M
Biochimica et biophysica acta 2013 Aug;1833(8):1820-31
Biochimica et biophysica acta 2013 Aug;1833(8):1820-31
Tumor suppressor Nf2 limits expansion of the neural progenitor pool by inhibiting Yap/Taz transcriptional coactivators.
Lavado A, He Y, Paré J, Neale G, Olson EN, Giovannini M, Cao X
Development (Cambridge, England) 2013 Aug;140(16):3323-34
Development (Cambridge, England) 2013 Aug;140(16):3323-34
FcγR-driven release of IL-6 by macrophages requires NOX2-dependent production of reactive oxygen species.
Franchini AM, Hunt D, Melendez JA, Drake JR
The Journal of biological chemistry 2013 Aug 30;288(35):25098-25108
The Journal of biological chemistry 2013 Aug 30;288(35):25098-25108
Spatial regulation of interleukin-6 signaling in response to neurodegenerative stressors in the retina.
Sims SM, Holmgren L, Cathcart HM, Sappington RM
American journal of neurodegenerative disease 2012;1(2):168-79
American journal of neurodegenerative disease 2012;1(2):168-79
Moloney murine leukemia virus genomic RNA packaged in the absence of a full complement of wild type nucleocapsid protein.
Johnson SF, Garcia EL, Summers MF, Telesnitsky A
Virology 2012 Sep 1;430(2):100-9
Virology 2012 Sep 1;430(2):100-9
Transient aggregation of ubiquitinated proteins is a cytosolic unfolded protein response to inflammation and endoplasmic reticulum stress.
Liu XD, Ko S, Xu Y, Fattah EA, Xiang Q, Jagannath C, Ishii T, Komatsu M, Eissa NT
The Journal of biological chemistry 2012 Jun 1;287(23):19687-98
The Journal of biological chemistry 2012 Jun 1;287(23):19687-98
Effect of cellular senescence on the albumin permeability of blood-derived endothelial cells.
Cheung TM, Ganatra MP, Peters EB, Truskey GA
American journal of physiology. Heart and circulatory physiology 2012 Dec 1;303(11):H1374-83
American journal of physiology. Heart and circulatory physiology 2012 Dec 1;303(11):H1374-83
Identification of cyclophilin-40-interacting proteins reveals potential cellular function of cyclophilin-40.
Park MS, Chu F, Xie J, Wang Y, Bhattacharya P, Chan WK
Analytical biochemistry 2011 Mar 15;410(2):257-65
Analytical biochemistry 2011 Mar 15;410(2):257-65
Beta-arrestin 2 is required for B1 receptor-dependent post-translational activation of inducible nitric oxide synthase.
Kuhr FK, Zhang Y, Brovkovych V, Skidgel RA
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 Jul;24(7):2475-83
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 Jul;24(7):2475-83
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Beta-Actin was performed by loading 20 µg of SH-SY5Y (lane1), U2OS (lane2), Raji (lane3), A549 (lane4), HEK-293 (lane5), HeLa (lane6), MCF7 (lane 7) and Jurkat (lane8) cell lysate using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800), and iBlot® Dry Blotting System (IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skim milk for 1 hour at room temperature. Beta-Actin was detected at ~42 kDa using Beta-Actin Mouse Monoclonal Antibody (Product # AM4302) at 0.5 µg/mL in 2.5 % skim milk at 4°C overnight on a rocking platform. Goat Anti-Mouse IgG - HRP Secondary Antibody (Product # 62-6520) at 1:4000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Beta-Actin was performed by loading 20 µg of SH-SY5Y (lane1), U2OS (lane2), Raji (lane3), A549 (lane4), HEK-293 (lane5), HeLa (lane6), MCF7 (lane 7) and Jurkat (lane8) cell lysate using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800), and iBlot® Dry Blotting System (IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skim milk for 1 hour at room temperature. Beta-Actin was detected at ~42 kDa using Beta-Actin Mouse Monoclonal Antibody (Product # AM4302) at 0.5 µg/mL in 2.5 % skim milk at 4°C overnight on a rocking platform. Goat Anti-Mouse IgG - HRP Secondary Antibody (Product # 62-6520) at 1:4000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockout of ACTB was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR889078_LV ) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of ACTB was performed by loading 30 µg of A-375 wild type (Lane 1), A-375 Cas9 (Lane 2), A-375 ACTB KO (Lane 3) whole cell extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with beta Actin Monoclonal Antibody (AC-15) (Product # AM4302, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:10,000 dilution) using the iBright™ FL1500 (Product # A44115). Chemiluminescent detection was performed usingSuperSignal™ West Dura Extended Duration Substrate (Product # 34076). Loss of signal upon CRISPR mediated knockout (KO) using the LentiArray™ CRISPR product line confirms that antibody is specific to ACTB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- An siRNA targeting beta-actin was labeled with Cy™3 using the Silencer™ siRNA Labeling Kit. The labeled siRNA was transfected into HeLa cells and cells were analyzed 96 hours later using a beta Actin monoclonal antibody (Product # AM4302). Green: beta-actin protein detected with anti-ß-actin (Ambion) and a FITC labeled secondary antibody. Red: Cy3 labeled siRNA. Blue: DAPI stained nuclei. (Cy™3 is a trademark of Amersham Biosciences.)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of Beta-actin was done on Jurkat cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Tritonª X-100 for 20 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were labeled with Beta-actin Mouse Monoclonal Antibody (AM4302, red histogram) or with mouse isotype control (pink histogram) at 1-3 µg/million cells in 2.5% BSA. After incubation at room temperature for 2-3 hours, the cells were labeled with Alexa Fluor¨ 488 Rabbit Anti-Mouse Secondary Antibody (A11059) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune¨ Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Stevia prevents the expression of proinflammatory cytokines in chronic liver damage. Protein levels of TNF- alpha (a), IL-1 beta (b), IL-6 (c), and IL-10 (d) in samples of liver tissue were determined by Western blot analysis of the control, CCl 4 -, CCl 4 + stevia-, and stevia-treated rats. beta -actin was used as a control. The values are expressed as the fold increase of OD normalized to the control group values (control = 1). Each bar represents the mean value of three rats +- SE ( n = 3). a P < 0.05 versus the control group; b P < 0.05 versus the CCl 4 group.
