MA5-15739-D550

antibody from Invitrogen Antibodies

Targeting: ACTB

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15739-D550

Provider

Invitrogen Antibodies

Product name

beta Actin Monoclonal Antibody (BA3R), DyLight™ 550

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

MA5-15739-D550 has been successfully used for Immunofluorescence. DyLight 550 has an excitation/emission of 562/576nm. For visualization of actin filaments by immunofluorescence, fixation and permeabilization with methanol is required.

Reactivity

Human, Mouse, Rat, Chicken/Avian, Rabbit

Host

Mouse

Conjugate

Yellow dye

Isotype

IgG

Antibody clone number

BA3R

Vial size

50 µL

Concentration

1 mg/mL

Storage

4° C

References

Submitted references

Estradiol signaling mediates gender difference in visceral adiposity via autophagy.

Tao Z, Zheng LD, Smith C, Luo J, Robinson A, Almeida FA, Wang Z, Olumi AF, Liu D, Cheng Z
Cell death & disease 2018 Feb 22;9(3):309

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NF-κB and IRF1 Induce Endogenous Retrovirus K Expression via Interferon-Stimulated Response Elements in Its 5' Long Terminal Repeat.

Manghera M, Ferguson-Parry J, Lin R, Douville RN
Journal of virology 2016 Oct 15;90(20):9338-49

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A comparison of genetically matched cell lines reveals the equivalence of human iPSCs and ESCs.

Choi J, Lee S, Mallard W, Clement K, Tagliazucchi GM, Lim H, Choi IY, Ferrari F, Tsankov AM, Pop R, Lee G, Rinn JL, Meissner A, Park PJ, Hochedlinger K
Nature biotechnology 2015 Nov;33(11):1173-81

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of beta-actin (red) in HeLa cells. Cells were fixed with either 4% paraformaldehyde (PFA) for 15 minutes at room temperature followed by permeabilization with 0.5% Triton X-100 in PBS for 15 minutes at room temperature (left panel) or fixed and permeabilized with ice-cold methanol for 10 minutes at room temperature (right panel). Cells were blocked with 0.3% BSA in PBS for 15 minutes, and probed with a DyLight 550-conjugated beta-actin monoclonal antibody (Product # MA5-15739-D550) at a dilution of 1:50 overnight at 4C. Nuclei (blue) were stained with DAPI (Product # 62247). Images were taken on a ToxInsight Instrument at 20X magnification.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 Differentially expressed genes (DEGs) between isogenic hESC and hiPSC lines (a) Venn diagram showing the number of genes consistently up- or down- regulated in 3 biological replicate hiPSC lines when compared to 3 biological replicate hESC GFP lines from the same genetic background. (FDR