Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [2]
- Other assay [10]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-15739-D680 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- beta Actin Loading Control Monoclonal Antibody (BA3R), DyLight™ 680
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- MA5-15739-D680 has been successfully used for Western blot. DyLight 680 has an excitation/emission of 692/712 nm.
- Reactivity
- Human, Mouse, Rat, Canine, Chicken/Avian, Rabbit
- Host
- Mouse
- Conjugate
- Near infrared dye
- Isotype
- IgG
- Antibody clone number
- BA3R
- Vial size
- 50 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
Submitted references CLEC14A deficiency exacerbates neuronal loss by increasing blood-brain barrier permeability and inflammation.
Estradiol signaling mediates gender difference in visceral adiposity via autophagy.
NF-κB and IRF1 Induce Endogenous Retrovirus K Expression via Interferon-Stimulated Response Elements in Its 5' Long Terminal Repeat.
A comparison of genetically matched cell lines reveals the equivalence of human iPSCs and ESCs.
Effects of adult exposure to bisphenol a on genes involved in the physiopathology of rat prefrontal cortex.
Amylin deposition in the brain: A second amyloid in Alzheimer disease?
Kim Y, Lee S, Zhang H, Lee S, Kim H, Kim Y, Won MH, Kim YM, Kwon YG
Journal of neuroinflammation 2020 Feb 4;17(1):48
Journal of neuroinflammation 2020 Feb 4;17(1):48
Estradiol signaling mediates gender difference in visceral adiposity via autophagy.
Tao Z, Zheng LD, Smith C, Luo J, Robinson A, Almeida FA, Wang Z, Olumi AF, Liu D, Cheng Z
Cell death & disease 2018 Feb 22;9(3):309
Cell death & disease 2018 Feb 22;9(3):309
NF-κB and IRF1 Induce Endogenous Retrovirus K Expression via Interferon-Stimulated Response Elements in Its 5' Long Terminal Repeat.
Manghera M, Ferguson-Parry J, Lin R, Douville RN
Journal of virology 2016 Oct 15;90(20):9338-49
Journal of virology 2016 Oct 15;90(20):9338-49
A comparison of genetically matched cell lines reveals the equivalence of human iPSCs and ESCs.
Choi J, Lee S, Mallard W, Clement K, Tagliazucchi GM, Lim H, Choi IY, Ferrari F, Tsankov AM, Pop R, Lee G, Rinn JL, Meissner A, Park PJ, Hochedlinger K
Nature biotechnology 2015 Nov;33(11):1173-81
Nature biotechnology 2015 Nov;33(11):1173-81
Effects of adult exposure to bisphenol a on genes involved in the physiopathology of rat prefrontal cortex.
Castro B, Sánchez P, Torres JM, Ortega E
PloS one 2013;8(9):e73584
PloS one 2013;8(9):e73584
Amylin deposition in the brain: A second amyloid in Alzheimer disease?
Jackson K, Barisone GA, Diaz E, Jin LW, DeCarli C, Despa F
Annals of neurology 2013 Oct;74(4):517-26
Annals of neurology 2013 Oct;74(4):517-26
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Beta-Actin was performed by loading 50 µg of various cell lysates per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a DyLight 680-conjugated Beta-Actin monoclonal antibody (Product # MA5-15739-D680) at a dilution of 1:1000 for 1 hour at room temperature on a rocking platform and washed in TBS-0.1% Tween-20. Detection was performed using the LI-COR Odyssey.
- Conjugate
- Near infrared dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of A549 (Lane 1), COS-7 (Lane 2), MDCK (Lane 3), C2C12 (Lane 4), MDA-MB-231 (Lane 5), PC-12 (Lane 6), RSC96 (Lane 7), tissue extracts of Mouse Lung (Lane 8) and Rat stomach (Lane 9). The blot was probed with beta Actin Loading Control Monoclonal Antibody (BA3R), DyLight 680 (Product # MA5-15739-D680, 1 µg/mL). A 42 kDa band corresponding to beta Actin was observed across the cell lines and tissues tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10% Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary antibody following blocking with 5 % skimmed milk. Fluorescent detection was performed using the Odyssey® Fc imaging system (Li-cor Biosciences).
- Conjugate
- Near infrared dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Near infrared dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Near infrared dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Near infrared dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Near infrared dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Near infrared dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Near infrared dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Near infrared dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Differentially expressed genes (DEGs) between isogenic hESC and hiPSC lines (a) Venn diagram showing the number of genes consistently up- or down- regulated in 3 biological replicate hiPSC lines when compared to 3 biological replicate hESC GFP lines from the same genetic background. (FDR
- Conjugate
- Near infrared dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 2 Loss of CLEC14A exacerbated cerebral injury with VEGFR2 activation in MCAO stroke model. a , c Images of whole brains and comparison of Evans blue dye (EB) leakage in brains (P8 and adult) after EB injection (intraperitoneal route) in WT and CLEC14A KO mice ( n = 6, each group). b , d The quantitative analysis of Evans blue (EB) dye extravasation using spectrophotometer, 24 h after intraperitoneal injection of EB dye. e TTC staining of brain slices in the sham, WT, and CLEC14A KO groups 24-h post ischemia-reperfusion. Severe infarction was present in the CLEC14A KO group. Scale bar = 1 mm. f Percentage of infarct volume in the WT and CLEC14A KO groups 24 h after ischemia-reperfusion. g Neurological scores of WT and CLEC14A KO mice were evaluated. h VEGFR2, pVEGFR2 protein expression in ipsilateral brain lysate after ischemia-reperfusion injury. i , j Quantification data of relative pVEGFR2 and VEGFR2 protein expression. n = 6 per group; * P < 0.05, ** P < 0.01, *** P < 0.001. The results are mean values and the error bars represent the mean +- SD
- Conjugate
- Near infrared dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 CLEC14A deletion attenuated tight junctional proteins in cerebral ischemic regions. a Immunofluorescence staining for Occludin, Claudin-5, ZO-1, and CD31 with DAPI in the ischemic cortex of WT and CLEC14A KO mice 24 h after ischemia-reperfusion. n = 6 per group. Scale bars: 20 mum. b Quantitative analysis of Occludin/CD31 ratio. c Quantitative analysis of Claudin-5/CD31 ratio. d Quantitative analysis of ZO-1/CD31 ratio. e , f Western blot analysis of tight junctional proteins from ischemic brain and quantitative graph. * P < 0.05, ** P < 0.01, and *** P < 0.001. The results are mean values and the error bars represent the mean +- SD
- Conjugate
- Near infrared dye
