Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoelectron microscopy [1]
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Validation data
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- Product number
- 643804 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#643804, RRID:AB_2565573
- Product name
- Alexa Fluor® 594 anti-β-actin
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- β-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because β-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting).
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Yellow dye
- Antibody clone number
- 2F1-1
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- MCF7 mammary gland cells were fixed with cold methanol for ten minutes and blocked with 5% FBS for 30 minutes. Then, the cells were stained with 2.5 µg/mL of anti β-actin (clone 2F1-1) Alexa Fluor® 594 (red) at room temperature for two hours. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
- Conjugate
- Yellow dye