Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- MAB8969 - Provider product page
- Provider
- R&D Systems
- Product name
- Human beta-Actin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human beta-Actin in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P60709
- Isotype
- IgG
- Antibody clone number
- 937210
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The protective effects of total paeony glycoside on ischemia/reperfusion injury in H9C2 cells via inhibition of the PI3K/Akt signaling pathway.
Effect of photodynamic therapy combined with Celecoxib on expression of cyclooxygenase-2 protein in HeLa cells.
Protective effect of KLF15 on vascular endothelial dysfunction induced by TNF‑α.
Shen P, Chen J, Pan M
Molecular medicine reports 2018 Sep;18(3):3332-3340
Molecular medicine reports 2018 Sep;18(3):3332-3340
Effect of photodynamic therapy combined with Celecoxib on expression of cyclooxygenase-2 protein in HeLa cells.
Mao Y, Wang L, Xu C, Han S
Oncology letters 2018 May;15(5):6599-6603
Oncology letters 2018 May;15(5):6599-6603
Protective effect of KLF15 on vascular endothelial dysfunction induced by TNF‑α.
Liu B, Xu L, Yu X, Li W, Sun X, Xiao S, Guo M, Wang H
Molecular medicine reports 2018 Aug;18(2):1987-1994
Molecular medicine reports 2018 Aug;18(2):1987-1994
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- beta-Actin in HeLa Human Cell Line. beta-Actin was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human beta-Actin Monoclonal Antibody (Catalog # MAB8969) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.