Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [4]
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Validation data
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- Product number
- NB600-501H - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-501H, RRID:AB_1216153
- Product name
- Mouse Monoclonal beta-Actin Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used. The epitope recognized by the antibody is resistant to formalin-fixing and paraffin-embedding.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Drosophila, Feline, Guinea Pig, Hamster, Porcine, Rabbit, Sheep, Simian, Zebrafish
- Host
- Mouse
- Conjugate
- Horseradish Peroxidase
- Antigen sequence
N-terminus of the beta isoform of a
ctin.- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C in the dark.
Submitted references Neuroprotective activity of leukemia inhibitory factor is relayed through myeloid zinc finger-1 in a rat model of stroke.
Overexpression of hypoxia-inducible factor prolyl hydoxylase-2 attenuates hypoxia-induced vascular endothelial growth factor expression in luteal cells.
Expression and contribution of the HIF-1α/VEGF signaling pathway to luteal development and function in pregnant rats.
Development of an experimental model to assess the bioavailability of zinc in practical piglet diets.
Activation of the NLRP1b inflammasome independently of ASC-mediated caspase-1 autoproteolysis and speck formation.
Negative regulation of the NLRP3 inflammasome by A20 protects against arthritis.
Palmitate induces transcriptional regulation of BACE1 and presenilin by STAT3 in neurons mediated by astrocytes.
Davis SM, Collier LA, Foran EA, Leonardo CC, Ajmo CT Jr, Pennypacker KR
Metabolic brain disease 2019 Apr;34(2):631-640
Metabolic brain disease 2019 Apr;34(2):631-640
Overexpression of hypoxia-inducible factor prolyl hydoxylase-2 attenuates hypoxia-induced vascular endothelial growth factor expression in luteal cells.
Zhang Z, Pang X, Tang Z, Yin D, Wang Z
Molecular medicine reports 2015 Sep;12(3):3809-3814
Molecular medicine reports 2015 Sep;12(3):3809-3814
Expression and contribution of the HIF-1α/VEGF signaling pathway to luteal development and function in pregnant rats.
Wu L, Zhang Z, Pan X, Wang Z
Molecular medicine reports 2015 Nov;12(5):7153-9
Molecular medicine reports 2015 Nov;12(5):7153-9
Development of an experimental model to assess the bioavailability of zinc in practical piglet diets.
Brugger D, Buffler M, Windisch W
Archives of animal nutrition 2014;68(2):73-92
Archives of animal nutrition 2014;68(2):73-92
Activation of the NLRP1b inflammasome independently of ASC-mediated caspase-1 autoproteolysis and speck formation.
Van Opdenbosch N, Gurung P, Vande Walle L, Fossoul A, Kanneganti TD, Lamkanfi M
Nature communications 2014;5:3209
Nature communications 2014;5:3209
Negative regulation of the NLRP3 inflammasome by A20 protects against arthritis.
Vande Walle L, Van Opdenbosch N, Jacques P, Fossoul A, Verheugen E, Vogel P, Beyaert R, Elewaut D, Kanneganti TD, van Loo G, Lamkanfi M
Nature 2014 Aug 7;512(7512):69-73
Nature 2014 Aug 7;512(7512):69-73
Palmitate induces transcriptional regulation of BACE1 and presenilin by STAT3 in neurons mediated by astrocytes.
Liu L, Martin R, Kohler G, Chan C
Experimental neurology 2013 Oct;248:482-90
Experimental neurology 2013 Oct;248:482-90
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: beta-Actin Antibody (AC-15) [HRP] [NB600-501H] - Simple Western lane view shows a specific band for Beta-Actin in 0.2 mg/ml of Hela, MCF-7, SH-SY5Y, & Jurkat lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: beta-Actin Antibody (AC-15) [HRP] [NB600-501H] - Electropherogram image(s) of corresponding Simple Western lane view. Beta-Actin antibody was used at 1:500 dilution on Hela, MCF-7, SH-SY5Y, & Jurkat lysate(s).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: beta-Actin Antibody (AC-15) [HRP] [NB600-501H] - Electropherogram image(s) of corresponding Simple Western lane view. Beta-Actin antibody was used at 1:100 dilution on Hela, MCF-7, SH-SY5Y, & Jurkat lysate(s).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: beta-Actin Antibody (AC-15) [HRP] [NB600-501H] - Analysis of beta-actin in NIH/3T3 and HEK293T cells using beta-actin antibody conjugated with HRP. Image from verified customer review.