Antibody data
- Antibody Data
- Antigen structure
- References [14]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- MAB8929 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat beta-Actin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human, mouse, and rat beta-Actin in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P60709
- Isotype
- IgG
- Antibody clone number
- 937215
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Royal jelly attenuates cadmium-induced nephrotoxicity in male mice.
DNA methylation affects metastasis of renal cancer and is associated with TGF-β/RUNX3 inhibition.
A homozygous loss-of-function mutation leading to CYBC1 deficiency causes chronic granulomatous disease.
B-cell lymphoma 2 is associated with advanced tumor grade and clinical stage, and reduced overall survival in young Chinese patients with colorectal carcinoma.
Association between circadian gene CLOCK and cisplatin resistance in ovarian cancer cells: A preliminary study.
Memantine protects against ischemia/reperfusion-induced brain endothelial permeability.
Pharmacological Inhibition of Macrophage Toll-like Receptor 4/Nuclear Factor-kappa B Alleviates Rhabdomyolysis-induced Acute Kidney Injury.
Hochu-ekki-to Treatment Improves Reproductive and Immune Modulation in the Stress-Induced Rat Model of Polycystic Ovarian Syndrome.
Anti-neoplastic drugs increase caveolin-1-dependent migration, invasion and metastasis of cancer cells.
Cytoprotective Effects and Mechanisms of Δ-17 Fatty Acid Desaturase in Injured Human Umbilical Vein Endothelial Cells (HUVECs).
Cytoprotective Effects and Mechanisms of Δ-17 Fatty Acid Desaturase in Injured Human Umbilical Vein Endothelial Cells (HUVECs).
Microgravity Induction of TRAIL Expression in Preosteoclast Cells Enhances Osteoclast Differentiation.
Fatty acid-binding protein E-FABP restricts tumor growth by promoting IFN-β responses in tumor-associated macrophages.
Crosstalk between monocytes and renal mesangial cells via interaction of metalloproteinases and fractalkine in diabetic nephropathy.
Almeer RS, AlBasher GI, Alarifi S, Alkahtani S, Ali D, Abdel Moneim AE
Scientific reports 2019 Apr 9;9(1):5825
Scientific reports 2019 Apr 9;9(1):5825
DNA methylation affects metastasis of renal cancer and is associated with TGF-β/RUNX3 inhibition.
Zheng J, Mei Y, Xiang P, Zhai G, Zhao N, Xu C, Liu M, Pan Z, Tang K, Jia D
Cancer cell international 2018;18:56
Cancer cell international 2018;18:56
A homozygous loss-of-function mutation leading to CYBC1 deficiency causes chronic granulomatous disease.
Arnadottir GA, Norddahl GL, Gudmundsdottir S, Agustsdottir AB, Sigurdsson S, Jensson BO, Bjarnadottir K, Theodors F, Benonisdottir S, Ivarsdottir EV, Oddsson A, Kristjansson RP, Sulem G, Alexandersson KF, Juliusdottir T, Gudmundsson KR, Saemundsdottir J, Jonasdottir A, Jonasdottir A, Sigurdsson A, Manzanillo P, Gudjonsson SA, Thorisson GA, Magnusson OT, Masson G, Orvar KB, Holm H, Bjornsson S, Arngrimsson R, Gudbjartsson DF, Thorsteinsdottir U, Jonsdottir I, Haraldsson A, Sulem P, Stefansson K
Nature communications 2018 Oct 25;9(1):4447
Nature communications 2018 Oct 25;9(1):4447
B-cell lymphoma 2 is associated with advanced tumor grade and clinical stage, and reduced overall survival in young Chinese patients with colorectal carcinoma.
Wang J, He G, Yang Q, Bai L, Jian B, Li Q, Li Z
Oncology letters 2018 Jun;15(6):9009-9016
Oncology letters 2018 Jun;15(6):9009-9016
Association between circadian gene CLOCK and cisplatin resistance in ovarian cancer cells: A preliminary study.
Xu H, Wang Z, Mo G, Chen H
Oncology letters 2018 Jun;15(6):8945-8950
Oncology letters 2018 Jun;15(6):8945-8950
Memantine protects against ischemia/reperfusion-induced brain endothelial permeability.
Liu Y, Huang Y, Xu Y, Qu P, Wang M
IUBMB life 2018 Apr;70(4):336-343
IUBMB life 2018 Apr;70(4):336-343
Pharmacological Inhibition of Macrophage Toll-like Receptor 4/Nuclear Factor-kappa B Alleviates Rhabdomyolysis-induced Acute Kidney Injury.
Huang RS, Zhou JJ, Feng YY, Shi M, Guo F, Gou SJ, Salerno S, Ma L, Fu P
Chinese medical journal 2017 Sep 20;130(18):2163-2169
Chinese medical journal 2017 Sep 20;130(18):2163-2169
Hochu-ekki-to Treatment Improves Reproductive and Immune Modulation in the Stress-Induced Rat Model of Polycystic Ovarian Syndrome.
Park E, Choi CW, Kim SJ, Kim YI, Sin S, Chu JP, Heo JY
Molecules (Basel, Switzerland) 2017 Jun 13;22(6)
Molecules (Basel, Switzerland) 2017 Jun 13;22(6)
Anti-neoplastic drugs increase caveolin-1-dependent migration, invasion and metastasis of cancer cells.
Díaz-Valdivia NI, Calderón CC, Díaz JE, Lobos-González L, Sepulveda H, Ortíz RJ, Martinez S, Silva V, Maldonado HJ, Silva P, Wehinger S, Burzio VA, Torres VA, Montecino M, Leyton L, Quest AFG
Oncotarget 2017 Dec 19;8(67):111943-111965
Oncotarget 2017 Dec 19;8(67):111943-111965
Cytoprotective Effects and Mechanisms of Δ-17 Fatty Acid Desaturase in Injured Human Umbilical Vein Endothelial Cells (HUVECs).
Zhou H, Wang C
Medical science monitor : international medical journal of experimental and clinical research 2017 Apr 4;23:1627-1635
Medical science monitor : international medical journal of experimental and clinical research 2017 Apr 4;23:1627-1635
Cytoprotective Effects and Mechanisms of Δ-17 Fatty Acid Desaturase in Injured Human Umbilical Vein Endothelial Cells (HUVECs).
Zhou H, Wang C
Medical science monitor : international medical journal of experimental and clinical research 2017 Apr 4;23:1627-1635
Medical science monitor : international medical journal of experimental and clinical research 2017 Apr 4;23:1627-1635
Microgravity Induction of TRAIL Expression in Preosteoclast Cells Enhances Osteoclast Differentiation.
Sambandam Y, Baird KL, Stroebel M, Kowal E, Balasubramanian S, Reddy SV
Scientific reports 2016 May 4;6:25143
Scientific reports 2016 May 4;6:25143
Fatty acid-binding protein E-FABP restricts tumor growth by promoting IFN-β responses in tumor-associated macrophages.
Zhang Y, Sun Y, Rao E, Yan F, Li Q, Zhang Y, Silverstein KA, Liu S, Sauter E, Cleary MP, Li B
Cancer research 2014 Jun 1;74(11):2986-98
Cancer research 2014 Jun 1;74(11):2986-98
Crosstalk between monocytes and renal mesangial cells via interaction of metalloproteinases and fractalkine in diabetic nephropathy.
Wang Y, Wei Q, Liu Q, Li Z, Zhou L, Zou F, Yuan Y, Sun Z
Molecular medicine reports 2013 Dec;8(6):1817-23
Molecular medicine reports 2013 Dec;8(6):1817-23
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat beta-Actin by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.01 µg/mL of Mouse Anti-Human/Mouse/Rat beta-Actin Monoclonal Antibody (Catalog # MAB8929) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for beta-Actin at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human beta-Actin by Simple WesternTM. Simple Western lane view shows lysate of A431 human epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for beta-Actin at approximately 49 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human/Mouse/Rat beta-Actin Monoclonal Antibody (Catalog # MAB8929). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- beta-Actin in NIH-3T3 Mouse Cell Line. beta-Actin was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using Mouse Anti-Human/Mouse/Rat beta-Actin Monoclonal Antibody (Catalog # MAB8929) at 0.1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Cells were fixated in methanol. Specific staining was localized to cytoskeleton.