Antibody data
- Antibody Data
- Antigen structure
- References [13]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB600-505 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-505, RRID:AB_10000628
- Product name
- Rabbit Polyclonal beta-Actin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat, Bovine, Goat, Guinea Pig, Hamster, Porcine, Sheep
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references In Vitro and In Vivo Characterization of a Preclinical Irradiation-Adapted Model for Ewing Sarcoma.
Identification of an IL-1-induced gene expression pattern in AR(+) PCa cells that mimics the molecular phenotype of AR(-) PCa cells.
Development of matrix metalloproteinase-targeted probes for lung inflammation detection with positron emission tomography.
BRCA1-deficient breast cancer cell lines are resistant to MEK inhibitors and show distinct sensitivities to 6-thioguanine.
Functional characterization of the 12p12.1 renal cancer-susceptibility locus implicates BHLHE41.
Reversible infertility in a liver receptor homologue-1 (LRH-1)-knockdown mouse model.
IL-1β induces p62/SQSTM1 and represses androgen receptor expression in prostate cancer cells.
Suppression of BRCA1 sensitizes cells to proteasome inhibitors.
Regulation of septin dynamics by the Saccharomyces cerevisiae lysine acetyltransferase NuA4.
Persistent directional cell migration requires ion transport proteins as direction sensors and membrane potential differences in order to maintain directedness.
Quantitative proteomic analysis of PCSK9 gain of function in human hepatic HuH7 cells.
Tubulobulbar complexes are intercellular podosome-like structures that internalize intact intercellular junctions during epithelial remodeling events in the rat testis.
VEGF receptor phosphorylation status and apoptosis is modulated by a green tea component, epigallocatechin-3-gallate (EGCG), in B-cell chronic lymphocytic leukemia.
Shapiro MC, Tang T, Dasgupta A, Kurenbekova L, Shuck R, Gaber MW, Yustein JT
International journal of radiation oncology, biology, physics 2018 May 1;101(1):118-127
International journal of radiation oncology, biology, physics 2018 May 1;101(1):118-127
Identification of an IL-1-induced gene expression pattern in AR(+) PCa cells that mimics the molecular phenotype of AR(-) PCa cells.
Thomas-Jardin SE, Kanchwala MS, Jacob J, Merchant S, Meade RK, Gahnim NM, Nawas AF, Xing C, Delk NA
The Prostate 2018 Jun;78(8):595-606
The Prostate 2018 Jun;78(8):595-606
Development of matrix metalloproteinase-targeted probes for lung inflammation detection with positron emission tomography.
Kondo N, Temma T, Aita K, Shimochi S, Koshino K, Senda M, Iida H
Scientific reports 2018 Jan 22;8(1):1347
Scientific reports 2018 Jan 22;8(1):1347
BRCA1-deficient breast cancer cell lines are resistant to MEK inhibitors and show distinct sensitivities to 6-thioguanine.
Gu Y, Helenius M, Väänänen K, Bulanova D, Saarela J, Sokolenko A, Martens J, Imyanitov E, Kuznetsov S
Scientific reports 2016 Jun 17;6:28217
Scientific reports 2016 Jun 17;6:28217
Functional characterization of the 12p12.1 renal cancer-susceptibility locus implicates BHLHE41.
Bigot P, Colli LM, Machiela MJ, Jessop L, Myers TA, Carrouget J, Wagner S, Roberson D, Eymerit C, Henrion D, Chanock SJ
Nature communications 2016 Jul 7;7:12098
Nature communications 2016 Jul 7;7:12098
Reversible infertility in a liver receptor homologue-1 (LRH-1)-knockdown mouse model.
Gerrits H, Paradé MC, Koonen-Reemst AM, Bakker NE, Timmer-Hellings L, Sollewijn Gelpke MD, Gossen JA
Reproduction, fertility, and development 2014 Jan;26(2):293-306
Reproduction, fertility, and development 2014 Jan;26(2):293-306
IL-1β induces p62/SQSTM1 and represses androgen receptor expression in prostate cancer cells.
Chang MA, Patel V, Gwede M, Morgado M, Tomasevich K, Fong EL, Farach-Carson MC, Delk NA
Journal of cellular biochemistry 2014 Dec;115(12):2188-97
Journal of cellular biochemistry 2014 Dec;115(12):2188-97
Suppression of BRCA1 sensitizes cells to proteasome inhibitors.
Gu Y, Bouwman P, Greco D, Saarela J, Yadav B, Jonkers J, Kuznetsov SG
Cell death & disease 2014 Dec 18;5(12):e1580
Cell death & disease 2014 Dec 18;5(12):e1580
Regulation of septin dynamics by the Saccharomyces cerevisiae lysine acetyltransferase NuA4.
Mitchell L, Lau A, Lambert JP, Zhou H, Fong Y, Couture JF, Figeys D, Baetz K
PloS one 2011;6(10):e25336
PloS one 2011;6(10):e25336
Persistent directional cell migration requires ion transport proteins as direction sensors and membrane potential differences in order to maintain directedness.
Ozkucur N, Perike S, Sharma P, Funk RH
BMC cell biology 2011 Jan 22;12:4
BMC cell biology 2011 Jan 22;12:4
Quantitative proteomic analysis of PCSK9 gain of function in human hepatic HuH7 cells.
Denis N, Palmer-Smith H, Elisma F, Busuttil A, Wright TG, Bou Khalil M, Prat A, Seidah NG, Chrétien M, Mayne J, Figeys D
Journal of proteome research 2011 Apr 1;10(4):2011-26
Journal of proteome research 2011 Apr 1;10(4):2011-26
Tubulobulbar complexes are intercellular podosome-like structures that internalize intact intercellular junctions during epithelial remodeling events in the rat testis.
Young JS, Guttman JA, Vaid KS, Vogl AW
Biology of reproduction 2009 Jan;80(1):162-74
Biology of reproduction 2009 Jan;80(1):162-74
VEGF receptor phosphorylation status and apoptosis is modulated by a green tea component, epigallocatechin-3-gallate (EGCG), in B-cell chronic lymphocytic leukemia.
Lee YK, Bone ND, Strege AK, Shanafelt TD, Jelinek DF, Kay NE
Blood 2004 Aug 1;104(3):788-94
Blood 2004 Aug 1;104(3):788-94
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: beta-Actin Antibody [NB600-505] - analysis of Beta Actin on HeLa and LNCaP cell lysates(25ug/lane) using anti-Beta Actin antibody. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: beta-Actin Antibody [NB600-505] - Detection of actin Lane 1: HeLa cell lysate, Lane 2: rat brain, Lane 3: mouse brain, Lane 4: 3T3 cell lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: beta-Actin Antibody [NB600-505] - Lane 1: molecular weight. Lane 2: human embryonic kidney 293. Lane 3: human lung carcinoma A549. Lane 4: mouse brain. Load: 35 ug per lane. Primary antibody: Beta Actin antibody at 1:1,500 for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 42 kDa corresponding to beta Actin (arrowhead). Other band(s): none.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: beta-Actin Antibody [NB600-505] - Representative western blot bands: MMP-9, MMP-12 and beta-Actin in lung homogenates of the CS and Air groups from different gels. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-018-19890-1), licensed under a CC-BY licence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: beta-Actin Antibody [NB600-505] - Staining at 1:200 shows detection of actin at the neuromuscular juntion of rana pipiens tissue. Formalin fixed paraffin embedded sections.