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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- LS-C413493 - Provider product page
- Provider
- LSBio
- Product name
- ACTB / Beta Actin Antibody LS-C413493
- Antibody type
- Monoclonal
- Description
- Purified
- Reactivity
- Human, Mouse, Rat, Canine, Chicken/Avian, Rabbit, Simian
- Host
- Mouse
- Storage
- Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis of Beta-actin expression in 293T (A); HeLa (B); mouse kidney (C); rat heart (D); rat brain (E) whole cell lysates.
Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemical analysis of Beta-actin staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
