Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PAB9980 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB9980, RRID:AB_1671681
- Product name
- ACTB polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of ACTB.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Activation of the GLI oncogene through fusion with the beta-actin gene (ACTB) in a group of distinctive pericytic neoplasms: pericytoma with t(7;12).
Proteomic identification of brain proteins that interact with dynein light chain LC8.
A role for beta-actin in RNA polymerase III transcription.
Dahlén A, Fletcher CD, Mertens F, Fletcher JA, Perez-Atayde AR, Hicks MJ, Debiec-Rychter M, Sciot R, Wejde J, Wedin R, Mandahl N, Panagopoulos I
The American journal of pathology 2004 May;164(5):1645-53
The American journal of pathology 2004 May;164(5):1645-53
Proteomic identification of brain proteins that interact with dynein light chain LC8.
Navarro-Lérida I, Martínez Moreno M, Roncal F, Gavilanes F, Albar JP, Rodríguez-Crespo I
Proteomics 2004 Feb;4(2):339-46
Proteomics 2004 Feb;4(2):339-46
A role for beta-actin in RNA polymerase III transcription.
Hu P, Wu S, Hernandez N
Genes & development 2004 Dec 15;18(24):3010-5
Genes & development 2004 Dec 15;18(24):3010-5
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using ACTB polyclonal antibody (Cat # PAB9980) shows detection of a predominant band at ~42 KDa corresponding to ACTB (arrowhead) in various lysates.Lysates are as follows : HEK 293 (Lane 2), human lung carcinoma A-549 (Lane 3) and mouse brain (Lane 4) all using the 800 nm channel (green).Approximately 35 ug of each lysate was separated on a 4-20% Tris-glycine gel by SDS-PAGE and transferred onto nitrocellulose.After blocking the membrane was probed with the primary antibody diluted to 1 : 1,500.Incubation was overnight at 4°C followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.Molecular weight markers are shown in lane 1 using the 700 nm channel (red).IRDye™800 fluorescence image was captured using the Odyssey u Infrared Imaging System developed by LI-COR.IRDye is a trademark of LI-COR, Inc.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence staining of ACTB polyclonal antibody (Cat # PAB9980) at 1 : 200 is shown to detect actin at the neuromuscular junction of rana pipiens tissue (sections 4.2 um thick).Personal Communication. S. Moeckel-Cole, Univ. MA, Amherst, MA.
- Validation comment
- Immunofluorescence