Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [4]
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Validation data
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- Product number
- NBP2-54690 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal beta-Actin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human beta-Actin antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: beta-Actin Antibody [NBP2-54690] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Human cell line RT-4 Lane 3: Human cell line U-251 MG Lane 4: Human plasma Lane 5: Human Liver tissue Lane 6: Human Tonsil tissue
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: beta-Actin Antibody [NBP2-54690] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: beta-Actin Antibody [NBP2-54690] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: beta-Actin Antibody [NBP2-54690] - Analysis in human cell line U-138MG.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: beta-Actin Antibody [NBP2-54690] - Staining of human prostate shows moderate positivity in smooth muscle cells.
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- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: beta-Actin Antibody [NBP2-54690] - Staining of human tonsil shows strong positivity in lymphoid cells.
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- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: beta-Actin Antibody [NBP2-54690] - Staining of human fallopian tube shows moderate positivity in glandular cells.
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- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: beta-Actin Antibody [NBP2-54690] - Staining of human stomach shows moderate membranous positivity.