Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- STJ97814 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-ACTB antibody [8H10D10] (STJ97814)
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody anti-Actin-Cytoplasmic 1 is suitable for use in Western Blot, Immunofluorescence, Immunocytochemistry, Flow Cytometry and ELISA research applications.
- Reactivity
- Human, Mouse, Rat, Hamster, Simian
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NA
- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
- Handling
- NA
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Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Western blot analysis using Actin Beta monoclonal antibody against NIH/3T3 (1) , Jurkat (2) , HeLa (3) , CHO (4) , PC12 (5) , HEK293 (6) , COS (7) , A549 (8) and MCF-7 (9) cell lysate.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis of SKBR-3 (left) and A549 (right) cells using Actin Beta monoclonal antibody (red, the secondary Ab is Cy3-Goat anti mouse IgG). Blue: DRAQ5 fluorescent DNA dye.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MCF-7 cells using Actin Beta monoclonal antibody (right) and negative control (left).
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA