Ab175850

antibody from Aladdin Scientific

Targeting: ACTB

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

Ab175850

Provider

Aladdin Scientific

Product name

beta Actin Mouse mAb (Biotin)

Antibody type

Monoclonal

Description

Application:WB :1:5000-1:40000IF: 1:2000Flow: 1:500-1:2500

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Biotin

Isotype

IgG

Antibody clone number

FG01/2D10

Vial size

100_l,10_l,1ml,20_l,50_l

Concentration

1 mg/ml

Storage

Store at 4_ for one year. Avoid repeated freeze/thaw cycles. Protect from light.

Western blot

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

beta Actin Mouse mAb (Biotin) (Ab175850) - Western Blot All lanes: beta Actin Mouse mAb (Biotin) (Ab175850) at 1/5000 dilution Samples: Lysates at 20 µg per lane SA-HRP: 1:20000 Predicted band size: 42.0 kDa Observed band size: 42.0 kDa Exposure time: 12.1s

Immunocytochemistry

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

beta Actin Mouse mAb (Biotin) (Ab175850) - IF IF analysis of beta Actin (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Actin Mouse mAb (Biotin) (Ab175850) at 1/2000 dilution in blocking buffer overnight at 4°C. Then incubated with Recombinant Streptavidin Protein (Cy5) (rp169047) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Flow cytometry

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

beta Actin Mouse mAb (Biotin) (Ab175850) - Flow Cytometry Flow cytometry analysis of Beta Actin in Hela cells (red) compared to an isotype control (blue). The cells were fixed and permeabilized with 100% methanol for 5 minutes. Cells were stained with beta Actin Mouse mAb (Biotin) (Ab175850) at 1/100 dilution for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (Cy5) (rp169047) at a dilution of 1/2000 for 30 minutes at 4°C in the dark.