PA1-21167

antibody from Invitrogen Antibodies

Targeting: ACTB

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-21167

Provider

Invitrogen Antibodies

Product name

Anti-beta Actin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA1-211A detects thyroid receptor (TR) from human, mouse, rat and chicken samples. This antibody does not detect TRv alpha -2 or TR beta-1. PA1-211A has been successfully used in Western blot, IHC-P, ICC/IF and gel shift procedures. By Western blot, this antibody detects a ~48 kDa protein representing recombinant human TR alpha-1 expressed in E. coli. Gel shift experiments show that this product reacts with TR alpha-1 homodimers and TR alpha-1/retinoic X receptor (RXR) heterodimers. PA1-211A immunizing peptide corresponds to amino acid residues 403-410 from human TR alpha-1. This sequence is completely conserved between human and rat TR alpha-1.

Host

Rabbit

Isotype

IgG

Vial size

500 µL

Concentration

0.2 mg/ml

Storage

4° C, do not freeze

References

Submitted references

Inhibition of Inflammasome-Dependent Interleukin 1β Production by Streptococcal NAD⁺-Glycohydrolase: Evidence for Extracellular Activity.

Hancz D, Westerlund E, Bastiat-Sempe B, Sharma O, Valfridsson C, Meyer L, Love JF, O'Seaghdha M, Wessels MR, Persson JJ
mBio 2017 Jul 18;8(4)

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The Social Amoeba Dictyostelium discoideum Is Highly Resistant to Polyglutamine Aggregation.

Santarriaga S, Petersen A, Ndukwe K, Brandt A, Gerges N, Bruns Scaglione J, Scaglione KM
The Journal of biological chemistry 2015 Oct 16;290(42):25571-8

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Tau deletion impairs intracellular β-amyloid-42 clearance and leads to more extracellular plaque deposition in gene transfer models.

Lonskaya I, Hebron M, Chen W, Schachter J, Moussa C
Molecular neurodegeneration 2014 Nov 10;9:46

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Induction of hepatic apolipoprotein A-I gene expression by the isoflavones quercetin and isoquercetrin.

Haas MJ, Onstead-Haas LM, Szafran-Swietlik A, Kojanian H, Davis T, Armstrong P, Wong NC, Mooradian AD
Life sciences 2014 Aug 6;110(1):8-14

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of beta Actin was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Beta Actin Rabbit Polyclonal Antibody (Product # PA1-21167) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of beta Actin in human leiomyoma using a beta Actin polyclonal antibody (Product # PA1-21167).