AM06276SU-N

antibody from Acris Antibodies GmbH

Targeting: ACTB

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM06276SU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM06276SU-N, RRID:AB_11217046

Product name

anti Actin beta / ACTB

Antibody type

Monoclonal

Antigen

Synthetic peptide corresponding to amino-terminal residues of Human beta-Actin, conjugated to KLH.

Reactivity

Human, Mouse, Rat, Hamster, Simian

Host

Mouse

Isotype

IgG

Antibody clone number

8H10D10

Vial size

0.1 ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot analysis using beta-Actin antibody Cat.-No AM06276SU-N against NIH/3T3 (Lane 1), Jurkat (Lane 2), Hela (Lane 3), CHO (Lane 4), PC12 (Lane 5), HEK293 (Lane 6), COS (Lane 7), A549 (Lane 8) and MCF-7 (Lane 9) cell lysate.

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Confocal Immunofluorescence analysis of SKBR-3 (left) and A549 (right) cells using beta Actin antibody Cat.-No AM06276SU-N (red, the secondary Ab is Cy3-Goat anti Mouse IgG). Blue: DRAQ5 fluorescent DNA dye.

Flow cytometry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Flow Cytometric analysis of MCF-7 cells using beta Actin antibody Cat.-No AM06276SU-N (right) and Negative Control (left).