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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [43]
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- Validations
- Western blot [11]
- Immunohistochemistry [2]
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- Product number
- NB600-532 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-532, RRID:AB_10002039
- Product name
- Rabbit Polyclonal beta-Actin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Premature termination codon readthrough upregulates progranulin expression and improves lysosomal function in preclinical models of GRN deficiency.
Palmitic acid damages gut epithelium integrity and initiates inflammatory cytokine production.
Identification of a genomic enhancer that enforces proper apoptosis induction in thymic negative selection.
Chronic Inflammation in Response to Injury: Retention of Myeloid Cells in Injured Tissue Is Driven by Myeloid Cell Intrinsic Factors.
Ponatinib Activates an Inflammatory Response in Endothelial Cells via ERK5 SUMOylation.
Poliovirus induces autophagic signaling independent of the ULK1 complex.
Cell cycle-dependent and -independent telomere shortening accompanies murine brain aging.
Lipid droplets induced by secreted phospholipase A2 and unsaturated fatty acids protect breast cancer cells from nutrient and lipotoxic stress.
ZNF341 controls STAT3 expression and thereby immunocompetence.
Translational control of tumor immune escape via the eIF4F-STAT1-PD-L1 axis in melanoma.
Nano-sized Al2O3 particle-induced autophagy reduces osteolysis in aseptic loosening of total hip arthroplasty by negative feedback regulation of RANKL expression in fibroblasts.
miR‑30a‑5p in the tumorigenesis of renal cell carcinoma: A tumor suppressive microRNA.
RNA helicase DDX3: a novel therapeutic target in Ewing sarcoma.
Enhanced ethanol catabolism in orphan nuclear receptor SHP-null mice.
Combinatorial Treatment with Apelin-13 Enhances the Therapeutic Efficacy of a Preconditioned Cell-Based Therapy for Peripheral Ischemia.
The Involvment of Hematopoietic-Specific PLC -β2 in Homing and Engraftment of Hematopoietic Stem/Progenitor Cells.
Increased plasma VEGF levels following ischemic preconditioning are associated with downregulation of miRNA-762 and miR-3072-5p.
Oncogenic cAMP responsive element binding protein 1 is overexpressed upon loss of tumor suppressive miR-10b-5p and miR-363-3p in renal cancer.
Cardiosphere-derived cell sheet primed with hypoxia improves left ventricular function of chronically infarcted heart.
MicroRNA-509-3p inhibits cancer cell proliferation and migration by targeting the mitogen-activated protein kinase kinase kinase 8 oncogene in renal cell carcinoma.
Intranasal deferoxamine engages multiple pathways to decrease memory loss in the APP/PS1 model of amyloid accumulation.
Marrow adipocyte-derived CXCL1 and CXCL2 contribute to osteolysis in metastatic prostate cancer.
Highly pathogenic H5N1 influenza A virus strains provoke heterogeneous IFN-α/β responses that distinctively affect viral propagation in human cells.
Antitumor Effect of Periplocin in TRAIL-Resistant Human Hepatocellular Carcinoma Cells through Downregulation of IAPs.
Human adipose microRNA-221 is upregulated in obesity and affects fat metabolism downstream of leptin and TNF-α.
IbACP, a sixteen-amino-acid peptide isolated from Ipomoea batatas leaves, induces carcinoma cell apoptosis.
Group X secreted phospholipase A(2) induces lipid droplet formation and prolongs breast cancer cell survival.
Krüppel-like factor 10 upregulates the expression of cyclooxygenase 1 and further modulates angiogenesis in endothelial cell and platelet aggregation in gene-deficient mice.
Intranasal deferoxamine improves performance in radial arm water maze, stabilizes HIF-1α, and phosphorylates GSK3β in P301L tau transgenic mice.
Krüppel-like factor 10 expression as a prognostic indicator for pancreatic adenocarcinoma.
"Autophagic flux" in normal mouse tissues: focus on endogenous LC3A processing.
Gammaherpesvirus gene expression and DNA synthesis are facilitated by viral protein kinase and histone variant H2AX.
Lung autophagic response following exposure of mice to whole body irradiation, with and without amifostine.
The human lung adenocarcinoma cell line EKVX produces an infectious xenotropic murine leukemia virus.
Klf10 induces cell apoptosis through modulation of BI-1 expression and Ca2+ homeostasis in estrogen-responding adenocarcinoma cells.
Aberrant regulation of pVHL levels by microRNA promotes the HIF/VEGF axis in CLL B cells.
Twinkle mutations associated with autosomal dominant progressive external ophthalmoplegia lead to impaired helicase function and in vivo mtDNA replication stalling.
Preferential killing of breast tumor initiating cells by N,N-diethyl-2-[4-(phenylmethyl)phenoxy]ethanamine/tesmilifene.
Effects of dietary decosahexaenoic acid (DHA) on eNOS in human coronary artery endothelial cells.
Small interfering RNA profiling reveals key role of clathrin-mediated endocytosis and early endosome formation for infection by respiratory syncytial virus.
MicroRNAs regulate the expression of the alternative splicing factor nPTB during muscle development.
Localization and role of NPC1L1 in cholesterol absorption in human intestine.
Genome-wide discovery of loci influencing chemotherapy cytotoxicity.
Frew J, Baradaran-Heravi A, Balgi AD, Wu X, Yan TD, Arns S, Shidmoossavee FS, Tan J, Jaquith JB, Jansen-West KR, Lynn FC, Gao FB, Petrucelli L, Feldman HH, Mackenzie IR, Roberge M, Nygaard HB
Molecular neurodegeneration 2020 Mar 16;15(1):21
Molecular neurodegeneration 2020 Mar 16;15(1):21
Palmitic acid damages gut epithelium integrity and initiates inflammatory cytokine production.
Ghezzal S, Postal BG, Quevrain E, Brot L, Seksik P, Leturque A, Thenet S, Carrière V
Biochimica et biophysica acta. Molecular and cell biology of lipids 2020 Feb;1865(2):158530
Biochimica et biophysica acta. Molecular and cell biology of lipids 2020 Feb;1865(2):158530
Identification of a genomic enhancer that enforces proper apoptosis induction in thymic negative selection.
Hojo MA, Masuda K, Hojo H, Nagahata Y, Yasuda K, Ohara D, Takeuchi Y, Hirota K, Suzuki Y, Kawamoto H, Kawaoka S
Nature communications 2019 Jun 13;10(1):2603
Nature communications 2019 Jun 13;10(1):2603
Chronic Inflammation in Response to Injury: Retention of Myeloid Cells in Injured Tissue Is Driven by Myeloid Cell Intrinsic Factors.
Torbica T, Wicks K, Umehara T, Gungordu L, Alrdahe S, Wemyss K, Grainger JR, Mace KA
The Journal of investigative dermatology 2019 Jul;139(7):1583-1592
The Journal of investigative dermatology 2019 Jul;139(7):1583-1592
Ponatinib Activates an Inflammatory Response in Endothelial Cells via ERK5 SUMOylation.
Paez-Mayorga J, Chen AL, Kotla S, Tao Y, Abe RJ, He ED, Danysh BP, Hofmann MC, Le NT
Frontiers in cardiovascular medicine 2018;5:125
Frontiers in cardiovascular medicine 2018;5:125
Poliovirus induces autophagic signaling independent of the ULK1 complex.
Corona Velazquez A, Corona AK, Klein KA, Jackson WT
Autophagy 2018;14(7):1201-1213
Autophagy 2018;14(7):1201-1213
Cell cycle-dependent and -independent telomere shortening accompanies murine brain aging.
Ain Q, Schmeer C, Penndorf D, Fischer M, Bondeva T, Förster M, Haenold R, Witte OW, Kretz A
Aging 2018 Nov 20;10(11):3397-3420
Aging 2018 Nov 20;10(11):3397-3420
Lipid droplets induced by secreted phospholipase A2 and unsaturated fatty acids protect breast cancer cells from nutrient and lipotoxic stress.
Jarc E, Kump A, Malavašič P, Eichmann TO, Zimmermann R, Petan T
Biochimica et biophysica acta. Molecular and cell biology of lipids 2018 Mar;1863(3):247-265
Biochimica et biophysica acta. Molecular and cell biology of lipids 2018 Mar;1863(3):247-265
ZNF341 controls STAT3 expression and thereby immunocompetence.
Frey-Jakobs S, Hartberger JM, Fliegauf M, Bossen C, Wehmeyer ML, Neubauer JC, Bulashevska A, Proietti M, Fröbel P, Nöltner C, Yang L, Rojas-Restrepo J, Langer N, Winzer S, Engelhardt KR, Glocker C, Pfeifer D, Klein A, Schäffer AA, Lagovsky I, Lachover-Roth I, Béziat V, Puel A, Casanova JL, Fleckenstein B, Weidinger S, Kilic SS, Garty BZ, Etzioni A, Grimbacher B
Science immunology 2018 Jun 15;3(24)
Science immunology 2018 Jun 15;3(24)
Translational control of tumor immune escape via the eIF4F-STAT1-PD-L1 axis in melanoma.
Cerezo M, Guemiri R, Druillennec S, Girault I, Malka-Mahieu H, Shen S, Allard D, Martineau S, Welsch C, Agoussi S, Estrada C, Adam J, Libenciuc C, Routier E, Roy S, Désaubry L, Eggermont AM, Sonenberg N, Scoazec JY, Eychène A, Vagner S, Robert C
Nature medicine 2018 Dec;24(12):1877-1886
Nature medicine 2018 Dec;24(12):1877-1886
Nano-sized Al2O3 particle-induced autophagy reduces osteolysis in aseptic loosening of total hip arthroplasty by negative feedback regulation of RANKL expression in fibroblasts.
Li, Wang C, Li Z, Wang H, He J, Zhu J, Zhang Y, Shen C, Xiao F, Gao Y, Zhang X, Li Y, Wang P, Peng J, Cai G, Zuo B, Yang Y, Shen Y, Song W, Zhang X, Shen L, Chen X
Cell death & disease 2018 Aug 6;9(8):840
Cell death & disease 2018 Aug 6;9(8):840
miR‑30a‑5p in the tumorigenesis of renal cell carcinoma: A tumor suppressive microRNA.
Li Y, Li Y, Chen D, Jin L, Su Z, Liu J, Duan H, Li X, Qi Z, Shi M, Ni L, Yang S, Gui Y, Mao X, Chen Y, Lai Y
Molecular medicine reports 2016 May;13(5):4085-94
Molecular medicine reports 2016 May;13(5):4085-94
RNA helicase DDX3: a novel therapeutic target in Ewing sarcoma.
Wilky BA, Kim C, McCarty G, Montgomery EA, Kammers K, DeVine LR, Cole RN, Raman V, Loeb DM
Oncogene 2016 May 19;35(20):2574-83
Oncogene 2016 May 19;35(20):2574-83
Enhanced ethanol catabolism in orphan nuclear receptor SHP-null mice.
Park JE, Lee M, Mifflin R, Lee YK
American journal of physiology. Gastrointestinal and liver physiology 2016 May 15;310(10):G799-807
American journal of physiology. Gastrointestinal and liver physiology 2016 May 15;310(10):G799-807
Combinatorial Treatment with Apelin-13 Enhances the Therapeutic Efficacy of a Preconditioned Cell-Based Therapy for Peripheral Ischemia.
Samura M, Morikage N, Suehiro K, Tanaka Y, Nakamura T, Nishimoto A, Ueno K, Hosoyama T, Hamano K
Scientific reports 2016 Jan 14;6:19379
Scientific reports 2016 Jan 14;6:19379
The Involvment of Hematopoietic-Specific PLC -β2 in Homing and Engraftment of Hematopoietic Stem/Progenitor Cells.
Adamiak M, Suszynska M, Abdel-Latif A, Abdelbaset-Ismail A, Ratajczak J, Ratajczak MZ
Stem cell reviews and reports 2016 Dec;12(6):613-620
Stem cell reviews and reports 2016 Dec;12(6):613-620
Increased plasma VEGF levels following ischemic preconditioning are associated with downregulation of miRNA-762 and miR-3072-5p.
Ueno K, Samura M, Nakamura T, Tanaka Y, Takeuchi Y, Kawamura D, Takahashi M, Hosoyama T, Morikage N, Hamano K
Scientific reports 2016 Dec 1;6:36758
Scientific reports 2016 Dec 1;6:36758
Oncogenic cAMP responsive element binding protein 1 is overexpressed upon loss of tumor suppressive miR-10b-5p and miR-363-3p in renal cancer.
Li Y, Chen D, Li Y, Jin L, Liu J, Su Z, Qi Z, Shi M, Jiang Z, Ni L, Yang S, Gui Y, Mao X, Chen Y, Lai Y
Oncology reports 2016 Apr;35(4):1967-78
Oncology reports 2016 Apr;35(4):1967-78
Cardiosphere-derived cell sheet primed with hypoxia improves left ventricular function of chronically infarcted heart.
Hosoyama T, Samura M, Kudo T, Nishimoto A, Ueno K, Murata T, Ohama T, Sato K, Mikamo A, Yoshimura K, Li TS, Hamano K
American journal of translational research 2015;7(12):2738-51
American journal of translational research 2015;7(12):2738-51
MicroRNA-509-3p inhibits cancer cell proliferation and migration by targeting the mitogen-activated protein kinase kinase kinase 8 oncogene in renal cell carcinoma.
Su Z, Chen D, Zhang E, Li Y, Yu Z, Shi M, Jiang Z, Ni L, Yang S, Gui Y, Ye J, Lai Y
Molecular medicine reports 2015 Jul;12(1):1535-43
Molecular medicine reports 2015 Jul;12(1):1535-43
Intranasal deferoxamine engages multiple pathways to decrease memory loss in the APP/PS1 model of amyloid accumulation.
Fine JM, Renner DB, Forsberg AC, Cameron RA, Galick BT, Le C, Conway PM, Stroebel BM, Frey WH 2nd, Hanson LR
Neuroscience letters 2015 Jan 1;584:362-7
Neuroscience letters 2015 Jan 1;584:362-7
Marrow adipocyte-derived CXCL1 and CXCL2 contribute to osteolysis in metastatic prostate cancer.
Hardaway AL, Herroon MK, Rajagurubandara E, Podgorski I
Clinical & experimental metastasis 2015 Apr;32(4):353-68
Clinical & experimental metastasis 2015 Apr;32(4):353-68
Highly pathogenic H5N1 influenza A virus strains provoke heterogeneous IFN-α/β responses that distinctively affect viral propagation in human cells.
Matthaei M, Budt M, Wolff T
PloS one 2013;8(2):e56659
PloS one 2013;8(2):e56659
Antitumor Effect of Periplocin in TRAIL-Resistant Human Hepatocellular Carcinoma Cells through Downregulation of IAPs.
Cheng CF, Lu IH, Tseng HW, Sun CY, Lin LT, Kuo ZK, Pan IH, Ko CH
Evidence-based complementary and alternative medicine : eCAM 2013;2013:958025
Evidence-based complementary and alternative medicine : eCAM 2013;2013:958025
Human adipose microRNA-221 is upregulated in obesity and affects fat metabolism downstream of leptin and TNF-α.
Meerson A, Traurig M, Ossowski V, Fleming JM, Mullins M, Baier LJ
Diabetologia 2013 Sep;56(9):1971-9
Diabetologia 2013 Sep;56(9):1971-9
IbACP, a sixteen-amino-acid peptide isolated from Ipomoea batatas leaves, induces carcinoma cell apoptosis.
Chang VH, Yang DH, Lin HH, Pearce G, Ryan CA, Chen YC
Peptides 2013 Sep;47:148-56
Peptides 2013 Sep;47:148-56
Group X secreted phospholipase A(2) induces lipid droplet formation and prolongs breast cancer cell survival.
Pucer A, Brglez V, Payré C, Pungerčar J, Lambeau G, Petan T
Molecular cancer 2013 Sep 27;12(1):111
Molecular cancer 2013 Sep 27;12(1):111
Krüppel-like factor 10 upregulates the expression of cyclooxygenase 1 and further modulates angiogenesis in endothelial cell and platelet aggregation in gene-deficient mice.
Yang DH, Hsu CF, Lin CY, Guo JY, Yu WC, Chang VH
The international journal of biochemistry & cell biology 2013 Feb;45(2):419-28
The international journal of biochemistry & cell biology 2013 Feb;45(2):419-28
Intranasal deferoxamine improves performance in radial arm water maze, stabilizes HIF-1α, and phosphorylates GSK3β in P301L tau transgenic mice.
Fine JM, Baillargeon AM, Renner DB, Hoerster NS, Tokarev J, Colton S, Pelleg A, Andrews A, Sparley KA, Krogh KM, Frey WH, Hanson LR
Experimental brain research 2012 Jun;219(3):381-90
Experimental brain research 2012 Jun;219(3):381-90
Krüppel-like factor 10 expression as a prognostic indicator for pancreatic adenocarcinoma.
Chang VH, Chu PY, Peng SL, Mao TL, Shan YS, Hsu CF, Lin CY, Tsai KK, Yu WC, Ch'ang HJ
The American journal of pathology 2012 Aug;181(2):423-30
The American journal of pathology 2012 Aug;181(2):423-30
"Autophagic flux" in normal mouse tissues: focus on endogenous LC3A processing.
Zois CE, Giatromanolaki A, Sivridis E, Papaiakovou M, Kainulainen H, Koukourakis MI
Autophagy 2011 Nov;7(11):1371-8
Autophagy 2011 Nov;7(11):1371-8
Gammaherpesvirus gene expression and DNA synthesis are facilitated by viral protein kinase and histone variant H2AX.
Mounce BC, Tsan FC, Droit L, Kohler S, Reitsma JM, Cirillo LA, Tarakanova VL
Virology 2011 Nov 25;420(2):73-81
Virology 2011 Nov 25;420(2):73-81
Lung autophagic response following exposure of mice to whole body irradiation, with and without amifostine.
Zois CE, Giatromanolaki A, Kainulainen H, Botaitis S, Torvinen S, Simopoulos C, Kortsaris A, Sivridis E, Koukourakis MI
Biochemical and biophysical research communications 2011 Jan 7;404(1):552-8
Biochemical and biophysical research communications 2011 Jan 7;404(1):552-8
The human lung adenocarcinoma cell line EKVX produces an infectious xenotropic murine leukemia virus.
Cmarik JL, Troxler JA, Hanson CA, Zhang X, Ruscetti SK
Viruses 2011 Dec;3(12):2442-61
Viruses 2011 Dec;3(12):2442-61
Klf10 induces cell apoptosis through modulation of BI-1 expression and Ca2+ homeostasis in estrogen-responding adenocarcinoma cells.
Hsu CF, Sui CL, Wu WC, Wang JJ, Yang DH, Chen YC, Yu WC, Chang HS
The international journal of biochemistry & cell biology 2011 Apr;43(4):666-73
The international journal of biochemistry & cell biology 2011 Apr;43(4):666-73
Aberrant regulation of pVHL levels by microRNA promotes the HIF/VEGF axis in CLL B cells.
Ghosh AK, Shanafelt TD, Cimmino A, Taccioli C, Volinia S, Liu CG, Calin GA, Croce CM, Chan DA, Giaccia AJ, Secreto C, Wellik LE, Lee YK, Mukhopadhyay D, Kay NE
Blood 2009 May 28;113(22):5568-74
Blood 2009 May 28;113(22):5568-74
Twinkle mutations associated with autosomal dominant progressive external ophthalmoplegia lead to impaired helicase function and in vivo mtDNA replication stalling.
Goffart S, Cooper HM, Tyynismaa H, Wanrooij S, Suomalainen A, Spelbrink JN
Human molecular genetics 2009 Jan 15;18(2):328-40
Human molecular genetics 2009 Jan 15;18(2):328-40
Preferential killing of breast tumor initiating cells by N,N-diethyl-2-[4-(phenylmethyl)phenoxy]ethanamine/tesmilifene.
Deng T, Liu JC, Pritchard KI, Eisen A, Zacksenhaus E
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Jan 1;15(1):119-30
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Jan 1;15(1):119-30
Effects of dietary decosahexaenoic acid (DHA) on eNOS in human coronary artery endothelial cells.
Stebbins CL, Stice JP, Hart CM, Mbai FN, Knowlton AA
Journal of cardiovascular pharmacology and therapeutics 2008 Dec;13(4):261-8
Journal of cardiovascular pharmacology and therapeutics 2008 Dec;13(4):261-8
Small interfering RNA profiling reveals key role of clathrin-mediated endocytosis and early endosome formation for infection by respiratory syncytial virus.
Kolokoltsov AA, Deniger D, Fleming EH, Roberts NJ Jr, Karpilow JM, Davey RA
Journal of virology 2007 Jul;81(14):7786-800
Journal of virology 2007 Jul;81(14):7786-800
MicroRNAs regulate the expression of the alternative splicing factor nPTB during muscle development.
Boutz PL, Chawla G, Stoilov P, Black DL
Genes & development 2007 Jan 1;21(1):71-84
Genes & development 2007 Jan 1;21(1):71-84
Localization and role of NPC1L1 in cholesterol absorption in human intestine.
Sané AT, Sinnett D, Delvin E, Bendayan M, Marcil V, Ménard D, Beaulieu JF, Levy E
Journal of lipid research 2006 Oct;47(10):2112-20
Journal of lipid research 2006 Oct;47(10):2112-20
Genome-wide discovery of loci influencing chemotherapy cytotoxicity.
Watters JW, Kraja A, Meucci MA, Province MA, McLeod HL
Proceedings of the National Academy of Sciences of the United States of America 2004 Aug 10;101(32):11809-14
Proceedings of the National Academy of Sciences of the United States of America 2004 Aug 10;101(32):11809-14
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: beta-Actin Antibody [NB600-532] - Simple Western lane view shows a specific band for Beta Actin in 0.05 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: beta-Actin Antibody [NB600-532] - Detection of Beta Actin. Lane 1: nuclear extract from mouse bone marrow derived macrophages (5ug). Lane 2: cytoplasmic extract from mouse bone narrow derived macrophages (10ug). Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: beta-Actin Antibody [NB600-532] - Detection of actin in 3T3 lysates (20ug). ECL detection 30 seconds. A specific band is seen using different dilutions: Lane 1 (1:15,000), Lane 2 (1:10,000), and Lane 3 (1:15,000).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: beta-Actin Antibody [NB600-532] - Western blot analysis of Actin (NB600-532) using HepG2 whole cell lysate [NBP1-42569].
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: beta-Actin Antibody [NB600-532] - Western blot analysis of Actin (NB600-532) using RCC4 whole cell lysate [NBP1-30412].
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: beta-Actin Antibody [NB600-532] - Analysis using the HRP conjugate of NB600-532. Detection of Beta Actin in MCF-7 cell lysate (20ug) using anti-Beta Actin antibody. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: beta-Actin Antibody [NB600-532] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cytoskeletal Actin antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: beta-Actin Antibody [NB600-532] - Detection of human and mouse Cytoskeletal Actin by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cytoskeletal Actin antibody NB600-532 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: beta-Actin Antibody [NB600-532] - Simple Western lane view shows a specific band for Beta Actin using NB600-532 at 1:200 in A431, C2C12 and C6 cell lysates. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: beta-Actin Antibody [NB600-532] - Hypoxic preconditioning increases PBMNC sensitivity to apelin-13 via upregulation of APJ expression, leading to growth factor secretion.(a) APJ protein expression was significantly increased in hypoxic PBMNCs. *p
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: beta-Actin Antibody [NB600-532] - Treatments of periplocin and/or TRAIL activate DR4, FADD, and proapoptotic proteins in HCC cells. The effect of periplocin treatment on the expression of DR4, DR5, and FADD was analyzed by western blot. Image collected and cropped by CiteAb from the following publication (http://www.hindawi.com/journals/ecam/2013/958025/), licensed under a CC-BY licence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: beta-Actin Antibody [NB600-532] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-Cytoskeletal Actin used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: beta-Actin Antibody [NB600-532] - Detection of human Cytoskeletal Actin by immunohistochemistry. Sample: FFPE section of human ovarian cancer. Antibody: Affinity purified rabbit anti-Cytoskeletal Actin (NB600-532). Detection: DAB
