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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
- Flow cytometry [2]
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- Product number
- MA5-17706 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD21 Monoclonal Antibody (21A/5)
- Antibody type
- Monoclonal
- Description
- MA5-17706 targets CD21/CR2 in FACS and IHC (F) applications and shows reactivity with Human samples. The MA5-17706 immunogen is mouse monoclonal antibodies raised against human CD21.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 21A/5
- Vial size
- 200 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraformaldehyde-fixed paraffin-embedded human tonsil sections at 200x (A) and 400x (B) magnification with DAPI counterstain. Primary antibody used was a CD21/CR2 monoclonal antibody (clone 21A/5) at a 1:500 dilution, followed by secondary antibody was a 488 fluorescent conjugated Goat anti-mouse Ig at a 1:500 dilution and Normal Goat Serum (blocking)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Human Peripheral Blood Leukocytes stained using anti-CD21/CR2 Monoclonal Antibody (Product # MA5-17706) at a 0.5 µg/10^6 cells dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of human peripheral blood lymphocytes were stained using a CD21/CR2 monoclonal antibody (Product # MA5-17706) (filled histogram) or mouse IgG1 isotype control (open histogram).
