Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 12-0219-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD21 Monoclonal Antibody (HB5), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The HB5 monoclonal antibody reacts with human CD21, an approximately 145 kDa type I transmembrane protein. CD21 is expressed by mature B cells, folliculate dendritic cells and a subset of epithelial cells and is a receptor for complement component C3d and Epstein-Barr virus (EBV). CD21, in association with CD19 and CD81, participates in the multimolecular complex with BCR and is involved in B cell activation. Applications Reported: This HB5 antibody has been reported for use in flow cytometric analysis. Applications Tested: This HB5 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- HB5
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Epstein-Barr Virus Infection of Mammary Epithelial Cells Promotes Malignant Transformation.
Malaria-associated atypical memory B cells exhibit markedly reduced B cell receptor signaling and effector function.
DCLRE1C (ARTEMIS) mutations causing phenotypes ranging from atypical severe combined immunodeficiency to mere antibody deficiency.
Long-lived Plasmodium falciparum specific memory B cells in naturally exposed Swedish travelers.
Atypical and classical memory B cells produce Plasmodium falciparum neutralizing antibodies.
Chronic exposure to Plasmodium falciparum is associated with phenotypic evidence of B and T cell exhaustion.
Hu H, Luo ML, Desmedt C, Nabavi S, Yadegarynia S, Hong A, Konstantinopoulos PA, Gabrielson E, Hines-Boykin R, Pihan G, Yuan X, Sotiriou C, Dittmer DP, Fingeroth JD, Wulf GM
EBioMedicine 2016 Jul;9:148-160
EBioMedicine 2016 Jul;9:148-160
Malaria-associated atypical memory B cells exhibit markedly reduced B cell receptor signaling and effector function.
Portugal S, Tipton CM, Sohn H, Kone Y, Wang J, Li S, Skinner J, Virtaneva K, Sturdevant DE, Porcella SF, Doumbo OK, Doumbo S, Kayentao K, Ongoiba A, Traore B, Sanz I, Pierce SK, Crompton PD
eLife 2015 May 8;4
eLife 2015 May 8;4
DCLRE1C (ARTEMIS) mutations causing phenotypes ranging from atypical severe combined immunodeficiency to mere antibody deficiency.
Volk T, Pannicke U, Reisli I, Bulashevska A, Ritter J, Björkman A, Schäffer AA, Fliegauf M, Sayar EH, Salzer U, Fisch P, Pfeifer D, Di Virgilio M, Cao H, Yang F, Zimmermann K, Keles S, Caliskaner Z, Güner SÜ, Schindler D, Hammarström L, Rizzi M, Hummel M, Pan-Hammarström Q, Schwarz K, Grimbacher B
Human molecular genetics 2015 Dec 20;24(25):7361-72
Human molecular genetics 2015 Dec 20;24(25):7361-72
Long-lived Plasmodium falciparum specific memory B cells in naturally exposed Swedish travelers.
Ndungu FM, Lundblom K, Rono J, Illingworth J, Eriksson S, Färnert A
European journal of immunology 2013 Nov;43(11):2919-29
European journal of immunology 2013 Nov;43(11):2919-29
Atypical and classical memory B cells produce Plasmodium falciparum neutralizing antibodies.
Muellenbeck MF, Ueberheide B, Amulic B, Epp A, Fenyo D, Busse CE, Esen M, Theisen M, Mordmüller B, Wardemann H
The Journal of experimental medicine 2013 Feb 11;210(2):389-99
The Journal of experimental medicine 2013 Feb 11;210(2):389-99
Chronic exposure to Plasmodium falciparum is associated with phenotypic evidence of B and T cell exhaustion.
Illingworth J, Butler NS, Roetynck S, Mwacharo J, Pierce SK, Bejon P, Crompton PD, Marsh K, Ndungu FM
Journal of immunology (Baltimore, Md. : 1950) 2013 Feb 1;190(3):1038-47
Journal of immunology (Baltimore, Md. : 1950) 2013 Feb 1;190(3):1038-47
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD3 APC (Product # 17-0038-42) and Mouse IgG2a kappa Isotype Control PE (Product # 12-4724-81) (left) or PE Anti-Human CD21 (right). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Yellow dye