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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [10]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MA5-11417 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD21 Monoclonal Antibody (2G9)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- MA5-11417 targets CD21 in WB, FACS and IHC (P) applications and shows reactivity with Human samples. This antibody is not suitable for Raji cell lysates or mouse BAF-3 cell lysates in Western blot analysis. The MA5-11417 immunogen is recombinant protein encoding the external domain of human CD21.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2G9
- Vial size
- 500 µL
- Concentration
- Conc. Not Determined
- Storage
- 4° C
Submitted references Single-cell profiling of immune cells after neoadjuvant pembrolizumab and chemotherapy in IIIA non-small cell lung cancer (NSCLC).
IgM expression in paraffin sections distinguishes follicular lymphoma from reactive follicular hyperplasia.
Epstein-Barr virus-associated adrenal smooth muscle tumors and disseminated diffuse large B-cell lymphoma in a child with common variable immunodeficiency: a case report and review of the literature.
Occurrence of nodular lymphocyte-predominant hodgkin lymphoma in hermansky-pudlak type 2 syndrome is associated to natural killer and natural killer T cell defects.
CXCR5⁺ T helper cells mediate protective immunity against tuberculosis.
Folliculocentric B-cell-rich follicular dendritic cells sarcoma: a hitherto unreported morphological variant mimicking lymphoproliferative disorders.
The expression of IgM is helpful in the differentiation of primary cutaneous diffuse large B cell lymphoma and follicle center lymphoma.
Copy number alterations and expression profiles of candidate genes in a pulmonary inflammatory myofibroblastic tumor.
Diagnostic and differential diagnostic criteria of lymphoid neoplasms in bone marrow trephine biopsies: a study of 87 cases.
Identification of CXCL13 as a new marker for follicular dendritic cell sarcoma.
Hui Z, Zhang J, Ren Y, Li X, Yan C, Yu W, Wang T, Xiao S, Chen Y, Zhang R, Wei F, You J, Ren X
Cell death & disease 2022 Jul 13;13(7):607
Cell death & disease 2022 Jul 13;13(7):607
IgM expression in paraffin sections distinguishes follicular lymphoma from reactive follicular hyperplasia.
Zheng Y, Zhou X, Xie J, Zhu H, Zhang S, Zhang Y, Wei X, Yue B
International journal of clinical and experimental pathology 2014;7(6):3264-71
International journal of clinical and experimental pathology 2014;7(6):3264-71
Epstein-Barr virus-associated adrenal smooth muscle tumors and disseminated diffuse large B-cell lymphoma in a child with common variable immunodeficiency: a case report and review of the literature.
Petrilli G, Lorenzi L, Paracchini R, Ubiali A, Schumacher RF, Cabassa P, Facchetti F
International journal of surgical pathology 2014 Dec;22(8):712-21
International journal of surgical pathology 2014 Dec;22(8):712-21
Occurrence of nodular lymphocyte-predominant hodgkin lymphoma in hermansky-pudlak type 2 syndrome is associated to natural killer and natural killer T cell defects.
Lorenzi L, Tabellini G, Vermi W, Moratto D, Porta F, Notarangelo LD, Patrizi O, Sozzani S, de Saint Basile G, Latour S, Pace D, Lonardi S, Facchetti F, Badolato R, Parolini S
PloS one 2013;8(11):e80131
PloS one 2013;8(11):e80131
CXCR5⁺ T helper cells mediate protective immunity against tuberculosis.
Slight SR, Rangel-Moreno J, Gopal R, Lin Y, Fallert Junecko BA, Mehra S, Selman M, Becerril-Villanueva E, Baquera-Heredia J, Pavon L, Kaushal D, Reinhart TA, Randall TD, Khader SA
The Journal of clinical investigation 2013 Feb;123(2):712-26
The Journal of clinical investigation 2013 Feb;123(2):712-26
Folliculocentric B-cell-rich follicular dendritic cells sarcoma: a hitherto unreported morphological variant mimicking lymphoproliferative disorders.
Lorenzi L, Lonardi S, Petrilli G, Tanda F, Bella M, Laurino L, Rossi G, Facchetti F
Human pathology 2012 Feb;43(2):209-15
Human pathology 2012 Feb;43(2):209-15
The expression of IgM is helpful in the differentiation of primary cutaneous diffuse large B cell lymphoma and follicle center lymphoma.
Demirkesen C, Tüzüner N, Esen T, Lebe B, Ozkal S
Leukemia research 2011 Sep;35(9):1269-72
Leukemia research 2011 Sep;35(9):1269-72
Copy number alterations and expression profiles of candidate genes in a pulmonary inflammatory myofibroblastic tumor.
Jung SH, Yim SH, Hu HJ, Jung CK, Lee SH, Kim DH, Chung YJ
Lung cancer (Amsterdam, Netherlands) 2010 Nov;70(2):152-7
Lung cancer (Amsterdam, Netherlands) 2010 Nov;70(2):152-7
Diagnostic and differential diagnostic criteria of lymphoid neoplasms in bone marrow trephine biopsies: a study of 87 cases.
Horváth E, Mezei T, Pávai Z, Turcu M, Demian S, Tóth E, Chira L, Jung I
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie 2009;50(3):399-406
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie 2009;50(3):399-406
Identification of CXCL13 as a new marker for follicular dendritic cell sarcoma.
Vermi W, Lonardi S, Bosisio D, Uguccioni M, Danelon G, Pileri S, Fletcher C, Sozzani S, Zorzi F, Arrigoni G, Doglioni C, Ponzoni M, Facchetti F
The Journal of pathology 2008 Nov;216(3):356-64
The Journal of pathology 2008 Nov;216(3):356-64
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CD21 was performed by loading 25 µg of Daudi (Lane 1), Raji (Lane 2), and BAF-3 cell lysates (Lane 3) and a molecular weight protein ladder onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with a blocking buffer at 4ºC overnight. The membrane was probed with a CD21 monoclonal antibody (Product # MA5-11417) at a dilution of 1:100 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at 145 kDa in Daudi cells only.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD21 using a monoclonal antibody (Product # MA5-11417).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of CD21 in Daudi cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD21 monoclonal antibody (Product # MA5-11417) at a dilution of 1:2 for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.
