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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [4]
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- Product number
- NB100-63574 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-63574, RRID:AB_959953
- Product name
- Mouse Monoclonal CD21 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. NB100-63574 recognizes human CD21, a 145kD cell surface glycoprotein expressed by mature B lymphocytes, follicular dendritic cells, subsets of normal thymocytes and subsets of T cells. The CD21 molecule acts as a receptor for CD23, for complement components C3d, C3dg and iC3b and for Epstein Barr Virus. CD21 is part of the signalling complex that is linked to CD19, CD81 and phosphatidylinositol-3-kinase.
- Reactivity
- Human, Canine
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD21 Antibody (Bu33) [NB100-63574] - Using the Alexa Fluor 488 direct conjugate A surface stain was performed on human peripheral blood lymphocytes with CD21 (Bu33) antibody NB100-63574AF488 and a matched isotype control. Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-25196AF647.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD21 Antibody (Bu33) [NB100-63574] - Flow Cytometry: CD21 Antibody (Bu33) [Alexa Fluor 647] [NB100-63574AF647] - A surface stain was performed on human peripheral blood lymphocytes with CD21 (Bu33) antibody NB100-63574AF647 and a matched isotype control NBP2-27287AF647. Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-26646. Image using the Alexa Fluor 647 form of this antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD21 Antibody (Bu33) [NB100-63574] - Using the Allophycocyanin direct conjugate A cell surface stain was performed on Daudi cells with CD21 Antibody (Bu33) NB100-63574APC (blue) and a matched isotype control NBP2-27287APC (orange). Cells were incubated in an antibody dilution of 0.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to allophycocyanin.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD21 Antibody (Bu33) [NB100-63574] - Flow Cytometry: CD21 Antibody (Bu33) [Alexa Fluor 647] [NB100-63574AF647] - Immunophenotyping with Canine whole blood. image courtesy of customer. Image using the Alexa Fluor 647 form of this antibody.
