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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- BS4034 - Provider product page
- Provider
- Bioworld Technology, Inc
- Product name
- Cdc25B (phospho S323) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic phosphopeptide derived from human Cdc25B around the phosphorylation site of Serine 323.
- Description
- The activity of cyclin dependent kinases in cell cycle is regulated by the phosphorylation status, which is controlled by the antagonistic action of wee 1 kinase and Cdc25 phosphatases. Three Cdc25 genes are present in human cells: Cdc25A, Cdc25B and Cdc25C. Cdc25A and Cdc25B are expressed throughout the cell cycle, with peak expression in G1 for Cdc25A and in both G1 S phase and G2 for Cdc25B. Cdc25C is mainly expressed in G2 phase.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
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- Experimental details
- Western blot (WB) analysis of p-Cdc25B (S323) pAb at 1:500 dilutionLane1:Hela cell lysate treated with PMA(100nM,15mins)Lane2:sp2/0 cell lysate treated with PMA(100nM,15mins)Lane3:PC12 cell lysate treated with PMA(100nM,15mins)
