DCABH-1626

antibody from Creative Diagnostics

Targeting: CDC25B

Western blot (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

DCABH-1626

Provider

Creative Diagnostics

Proper citation

Creative Diagnostics Cat#DCABH-1626, RRID:AB_2475586

Product name

Rabbit Anti-Human Cdc25B monoclonal antibody, clone KK102-3

Antibody type

Monoclonal

Description

The Cdc2/cyclin B enzyme, involved in regulation of mitosis in eukaryotic cells, is subject to multiple levels of control. Among these, the regulation of the catalytic subunit by Tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/cyclin B complex, while Tyrosine dephosphorylation, which occurs at the onset of mitosis, directly activates the pre-MPH complex. The Cdc25 gene serves as a rate-limiting mitotic activator, apparently due to its action as the Cdc2 Tyrosine phosphatase. In the absence of Cdc25, Cdc2 accumulates in a Tyrosine phosphorylated state. In addition, Cdc25 proteins from a variety of species have been shown to share a low degree of sequence similarity with other Tyrosine phosphatases. The Cdc25 gene family consists of at least three members that share approximately 40% identity in their most conserved carboxy-terminal sequences.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

KK102-3

Vial size

100 _l

Storage

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.