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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB0433 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0433, RRID:AB_982429
- Product name
- CDC25B (phospho S353) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S353 of human CDC25B.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 400 μl
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Cdc25A and cdc25B expression in malignant lymphoma of the thyroid: correlation with histological subtypes and cell proliferation.
Nuclear export signal in CDC25B.
Coordinate expression of Cdc25B and ER-alpha is frequent in low-grade endometrioid endometrial carcinoma but uncommon in high-grade endometrioid and nonendometrioid carcinomas.
Ito Y, Yoshida H, Matsuzuka F, Matsuura N, Nakamura Y, Nakamine H, Kakudo K, Kuma K, Miyauchi A
International journal of molecular medicine 2004 Mar;13(3):431-5
International journal of molecular medicine 2004 Mar;13(3):431-5
Nuclear export signal in CDC25B.
Uchida S, Ohtsubo M, Shimura M, Hirata M, Nakagama H, Matsunaga T, Yoshida M, Ishizaka Y, Yamashita K
Biochemical and biophysical research communications 2004 Mar 26;316(1):226-32
Biochemical and biophysical research communications 2004 Mar 26;316(1):226-32
Coordinate expression of Cdc25B and ER-alpha is frequent in low-grade endometrioid endometrial carcinoma but uncommon in high-grade endometrioid and nonendometrioid carcinomas.
Wu W, Slomovitz BM, Celestino J, Chung L, Thornton A, Lu KH
Cancer research 2003 Oct 1;63(19):6195-9
Cancer research 2003 Oct 1;63(19):6195-9
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The CDC25B (phospho S353) polyclonal antibody (Cat # PAB0433) is used in Western blot to detect phospho-CDC25B-S353 in HepG2 cell lysate
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with CDC25B (phospho S353) polyclonal antibody (Cat # PAB0433) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
