Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00000994-B01P - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00000994-B01P, RRID:AB_10736427
- Product name
- CDC25B purified MaxPab mouse polyclonal antibody (B01P)
- Antibody type
- Polyclonal
- Description
- Mouse polyclonal antibody raised against a full-length human CDC25B protein.
- Antigen sequence
MEVPQPEPAPGSALSPAGVCGGAQRPGHLPGLLLG
SHGLLGSPVRAAASSPVTTLTQTMHDLAGLGSETP
KSQVGTLLFRSRSRLTHLSLSRRASESSLSSESSE
SSDAGLCMDSPSPMDPHMAEQTFEQAIQAASRIIR
NEQFAIRRFQSMPVRLLGHSPVLRNITNSQAPDGR
RKSEAGSGAASSSGEDKENDGFVFKMPWKPTHPSS
THALAEWASRREAFAQRPSSAPDLMCLSPDRKMEV
EELSPLALGRFSLTPAEGDTEEDDGFVDILESDLK
DDDAVPPGMESLISAPLVKTLEKEEEKDLVMYSKC
QRLFRSPSMPCSVIRPILKRLERPQDRDTPVQNKR
RRSVTPPEEQQEAEEPKARVLRSKSLCHDEIENLL
DSDHRELIGDYSKAFLLQTVDGKHQDLKYISPETM
VALLTGKFSNIVDKFVIVDCRYPYEYEGGHIKTAV
NLPLERDAESFLLKSPIAPCSLDKRVILIFHCEFS
SERGPRMCRFIRERDRAVNDYPSLYYPEMYILKGG
YKEFFPQHPNFCEPQDYRPMNHEAFKDELKTFRLK
TRSWAGERSRRELCSRLQDQ- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of CDC25B expression in transfected 293T cell line (H00000994-T01) by CDC25B MaxPab polyclonal antibody.Lane 1: CDC25B transfected lysate(63.8 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of purified MaxPab antibody to CDC25B on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol