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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Product number
- ABIN358101 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer353) antibody
- Antibody type
- Polyclonal
- Antigen
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S353 of human CDC25B.
- Description
- Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- pSer353
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Cdc25A and cdc25B expression in malignant lymphoma of the thyroid: correlation with histological subtypes and cell proliferation.
Nuclear export signal in CDC25B.
Coordinate expression of Cdc25B and ER-alpha is frequent in low-grade endometrioid endometrial carcinoma but uncommon in high-grade endometrioid and nonendometrioid carcinomas.
Protein kinase CK2 regulates CDC25B phosphatase activity.
LIM-only protein FHL3 interacts with CDC25B2 phosphatase.
Ito Y, Yoshida H, Matsuzuka F, Matsuura N, Nakamura Y, Nakamine H, Kakudo K, Kuma K, Miyauchi A
International journal of molecular medicine 2004 Mar;13(3):431-5
International journal of molecular medicine 2004 Mar;13(3):431-5
Nuclear export signal in CDC25B.
Uchida S, Ohtsubo M, Shimura M, Hirata M, Nakagama H, Matsunaga T, Yoshida M, Ishizaka Y, Yamashita K
Biochemical and biophysical research communications 2004 Mar 26;316(1):226-32
Biochemical and biophysical research communications 2004 Mar 26;316(1):226-32
Coordinate expression of Cdc25B and ER-alpha is frequent in low-grade endometrioid endometrial carcinoma but uncommon in high-grade endometrioid and nonendometrioid carcinomas.
Wu W, Slomovitz BM, Celestino J, Chung L, Thornton A, Lu KH
Cancer research 2003 Oct 1;63(19):6195-9
Cancer research 2003 Oct 1;63(19):6195-9
Protein kinase CK2 regulates CDC25B phosphatase activity.
Theis-Febvre N, Filhol O, Froment C, Cazales M, Cochet C, Monsarrat B, Ducommun B, Baldin V
Oncogene 2003 Jan 16;22(2):220-32
Oncogene 2003 Jan 16;22(2):220-32
LIM-only protein FHL3 interacts with CDC25B2 phosphatase.
Mils V, Lee SM, Joly W, Hang EW, Baldin V, Waye MM, Ducommun B, Tsui SK
Experimental cell research 2003 Apr 15;285(1):99-106
Experimental cell research 2003 Apr 15;285(1):99-106
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