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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- PA5-34670 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Clathrin Light Chain A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, HeLa, HepG2, HCT116, mouse brain, rat brain. Predicted reactivity: Mouse (98%), Xenopus laevis (84%), Chicken (89%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Clathrin Light Chain A was performed by separating 50 µg of various tissue extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a Clathrin Light Chain A Polyclonal Antibody (Product # PA5-34670) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Clathrin Light Chain A Polyclonal Antibody (Product # PA5-34670). Sample (30 µg of whole cell lysate). Lane A: A549. 12% SDS PAGE. Clathrin Light Chain A Polyclonal Antibody (Product # PA5-34670) diluted at 1:1,000.
