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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunohistochemistry [3]
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- Product number
- GTX103954 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX103954, RRID:AB_2036608
- Product name
- CNPase antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Molt-4 (GTX27912) 10% SDS PAGE GTX103954 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (20 ug of whole cell lysate) A: mouse brain 10% SDS PAGE GTX103954 diluted at 1:5000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various tissue extracts (50 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with CNP antibody (GTX103954) diluted at 1:5000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and CNPase knockout (KO) HeLa cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with CNPase antibody (GTX103954) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and CNPase knockout (KO) HeLa cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with CNPase antibody (GTX103954) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CNP antibody detects CNP protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. CNP antibody (GTX103954) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CNPase antibody detects CNPase protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse cerebellum. Green: CNPase protein stained by CNPase antibody (GTX103954) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CNPase antibody detects CNPase protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse hippocampus. Green: CNPase protein stained by CNPase antibody (GTX103954) diluted at 1:250.Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.
