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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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- Product number
- PA1-46433 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CNPase Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- This antibody is predicted to react with mouse and rat based on 94% sequence homology. Suggested positive control: antigen standard for CNP (transient overexpression lysate), normal human brain lysate.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CNPase with CNPase antibody of 1:500 dilution. 10 µg of normal brain tissue lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CNPase in 10 µg normal brain tissue lysate. Samples were incubated in CNPase polyclonal antibody (Product # PA1-46433) using a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-CNPase Polyclonal Antibody (Product # PA1-46433) and a 47 kDa band corresponding to CNPase was observed in Mouse brain and Mouse cerebellum when compared to Mouse Kidney, Mouse Lung and Mouse Colon which are reported to be low. CNPase is expressed exclusively by oligodendrocytes in the central nervous system. Membrane enriched extracts (30 µg lysate) of MOLT-4 (Lane 1), HeLa (Lane 2), SK-BR-3 (Lane 3), Hep G2 (Lane 4), tissue extracts of Mouse Brain (Lane 5), Mouse Cerebellum (Lane 6), Mouse Kidney (Lane 7), Mouse Colon (Lane 8) and Mouse Lung (Lane 9) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
