Antibody data
- Antibody Data
- Antigen structure
- References [21]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- 12895-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#12895-1-AP, RRID:AB_2086291
- Product name
- CUL1 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated CUL1 antibody (Cat. #12895-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IHC, IP, WB, ELISA.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references The UBE2F-CRL5(ASB11)-DIRAS2 axis is an oncogene and tumor suppressor cascade in pancreatic cancer cells.
A mitophagy sensor PPTC7 controls BNIP3 and NIX degradation to regulate mitochondrial mass.
Dysregulated NUB1 and Neddylation Enhances Rheumatoid Arthritis Fibroblast-Like Synoviocyte Inflammatory Responses.
Cullin-associated and neddylation-dissociated 1 regulate reprogramming of lipid metabolism through SKP1-Cullin-1-F-box(FBXO11) -mediated heterogeneous nuclear ribonucleoprotein A2/B1 ubiquitination and promote hepatocellular carcinoma.
Recruitment of FBXO22 for Targeted Degradation of NSD2.
FBXO38 mediates FGL1 ubiquitination and degradation to enhance cancer immunity and suppress inflammation.
A mitochondrial SCF-FBXL4 ubiquitin E3 ligase complex degrades BNIP3 and NIX to restrain mitophagy and prevent mitochondrial disease.
The tRNA-GCN2-FBXO22-axis-mediated mTOR ubiquitination senses amino acid insufficiency.
A small molecule inhibitor of the UBE2F-CRL5 axis induces apoptosis and radiosensitization in lung cancer.
A small-molecule Skp1 inhibitor elicits cell death by p53-dependent mechanism.
SKP2 drives the sensitivity to neddylation inhibitors and cisplatin in malignant pleural mesothelioma.
Early growth response-1 is a new substrate of the GSK3β-FBXW7 axis.
Cullin-associated and neddylation-dissociated 1 protein (CAND1) governs cardiac hypertrophy and heart failure partially through regulating calcineurin degradation.
Cardioprotective effect of MLN4924 on ameliorating autophagic flux impairment in myocardial ischemia-reperfusion injury by Sirt1.
The potential oncogenic and MLN4924-resistant effects of CSN5 on cervical cancer cells.
Let-7i-5p Regulation of Cell Morphology and Migration Through Distinct Signaling Pathways in Normal and Pathogenic Urethral Fibroblasts.
Nedd8-activating enzyme inhibitor MLN4924 (Pevonedistat), inhibits miR-1303 to suppress human breast cancer cell proliferation via targeting p27(Kip1).
Gossypol inhibits cullin neddylation by targeting SAG-CUL5 and RBX1-CUL1 complexes.
Inhibition of neddylation causes meiotic arrest in mouse oocyte.
The ubiquitin ligase SCF(FBXW7α) promotes GATA3 degradation.
Cullin-3-KCTD10-mediated CEP97 degradation promotes primary cilium formation.
Chang Y, Chen Q, Li H, Xu J, Tan M, Xiong X, Sun Y
Developmental cell 2024 May 20;59(10):1317-1332.e5
Developmental cell 2024 May 20;59(10):1317-1332.e5
A mitophagy sensor PPTC7 controls BNIP3 and NIX degradation to regulate mitochondrial mass.
Sun Y, Cao Y, Wan H, Memetimin A, Cao Y, Li L, Wu C, Wang M, Chen S, Li Q, Ma Y, Dong M, Jiang H
Molecular cell 2024 Jan 18;84(2):327-344.e9
Molecular cell 2024 Jan 18;84(2):327-344.e9
Dysregulated NUB1 and Neddylation Enhances Rheumatoid Arthritis Fibroblast-Like Synoviocyte Inflammatory Responses.
Sendo S, Machado CRL, Boyle DL, Benschop RJ, Perumal NB, Choi E, Wang W, Firestein GS
Arthritis & rheumatology (Hoboken, N.J.) 2024 Aug;76(8):1252-1262
Arthritis & rheumatology (Hoboken, N.J.) 2024 Aug;76(8):1252-1262
Cullin-associated and neddylation-dissociated 1 regulate reprogramming of lipid metabolism through SKP1-Cullin-1-F-box(FBXO11) -mediated heterogeneous nuclear ribonucleoprotein A2/B1 ubiquitination and promote hepatocellular carcinoma.
Zhang H, Xia P, Yang Z, Liu J, Zhu Y, Huang Z, Zhang Z, Yuan Y
Clinical and translational medicine 2023 Oct;13(10):e1443
Clinical and translational medicine 2023 Oct;13(10):e1443
Recruitment of FBXO22 for Targeted Degradation of NSD2.
Nie DY, Tabor JR, Li J, Kutera M, St-Germain J, Hanley RP, Wolf E, Paulakonis E, Kenney TMG, Duan S, Shrestha S, Owens DDG, Pon A, Szewczyk M, Lamberto AJ, Menes M, Li F, Barsyte-Lovejoy D, Brown NG, Barsotti AM, Stamford AW, Collins JL, Wilson DJ, Raught B, Licht JD, James LI, Arrowsmith CH
bioRxiv : the preprint server for biology 2023 Nov 30;
bioRxiv : the preprint server for biology 2023 Nov 30;
FBXO38 mediates FGL1 ubiquitination and degradation to enhance cancer immunity and suppress inflammation.
Tian T, Xie X, Yi W, Zhou Y, Xu Y, Wang Z, Zhang J, Lin M, Zhang R, Lv Z, Li X, Lv L, Xu Y
Cell reports 2023 Nov 28;42(11):113362
Cell reports 2023 Nov 28;42(11):113362
A mitochondrial SCF-FBXL4 ubiquitin E3 ligase complex degrades BNIP3 and NIX to restrain mitophagy and prevent mitochondrial disease.
Cao Y, Zheng J, Wan H, Sun Y, Fu S, Liu S, He B, Cai G, Cao Y, Huang H, Li Q, Ma Y, Chen S, Wang F, Jiang H
The EMBO journal 2023 Jul 3;42(13):e113033
The EMBO journal 2023 Jul 3;42(13):e113033
The tRNA-GCN2-FBXO22-axis-mediated mTOR ubiquitination senses amino acid insufficiency.
Ge MK, Zhang C, Zhang N, He P, Cai HY, Li S, Wu S, Chu XL, Zhang YX, Ma HM, Xia L, Yang S, Yu JX, Yao SY, Zhou XL, Su B, Chen GQ, Shen SM
Cell metabolism 2023 Dec 5;35(12):2216-2230.e8
Cell metabolism 2023 Dec 5;35(12):2216-2230.e8
A small molecule inhibitor of the UBE2F-CRL5 axis induces apoptosis and radiosensitization in lung cancer.
Xu T, Ma Q, Li Y, Yu Q, Pan P, Zheng Y, Li Z, Xiong X, Hou T, Yu B, Liu H, Sun Y
Signal transduction and targeted therapy 2022 Oct 17;7(1):354
Signal transduction and targeted therapy 2022 Oct 17;7(1):354
A small-molecule Skp1 inhibitor elicits cell death by p53-dependent mechanism.
Hussain M, Lu Y, Tariq M, Jiang H, Shu Y, Luo S, Zhu Q, Zhang J, Liu J
iScience 2022 Jul 15;25(7):104591
iScience 2022 Jul 15;25(7):104591
SKP2 drives the sensitivity to neddylation inhibitors and cisplatin in malignant pleural mesothelioma.
Salaroglio IC, Belisario DC, Bironzo P, Ananthanarayanan P, Ricci L, Digiovanni S, Fontana S, Napoli F, Sandri A, Facolmatà C, Libener R, Comunanza V, Grosso F, Gazzano E, Leo F, Taulli R, Bussolino F, Righi L, Papotti MG, Novello S, Scagliotti GV, Riganti C, Kopecka J
Journal of experimental & clinical cancer research : CR 2022 Feb 23;41(1):75
Journal of experimental & clinical cancer research : CR 2022 Feb 23;41(1):75
Early growth response-1 is a new substrate of the GSK3β-FBXW7 axis.
Yin L, Zhang J, Sun Y
Neoplasia (New York, N.Y.) 2022 Dec;34:100839
Neoplasia (New York, N.Y.) 2022 Dec;34:100839
Cullin-associated and neddylation-dissociated 1 protein (CAND1) governs cardiac hypertrophy and heart failure partially through regulating calcineurin degradation.
Li X, Zhang Y, Zhao Y, Zhou Y, Han Q, Yang Y, Zhang L, Shi L, Jin X, Zhang R, Gao H, Xue G, Li D, Zhang ZR, Lu Y, Yang B, Pan Z
Pharmacological research 2022 Aug;182:106284
Pharmacological research 2022 Aug;182:106284
Cardioprotective effect of MLN4924 on ameliorating autophagic flux impairment in myocardial ischemia-reperfusion injury by Sirt1.
Zhang J, Cui J, Zhao F, Yang L, Xu X, Shi Y, Wei B
Redox biology 2021 Oct;46:102114
Redox biology 2021 Oct;46:102114
The potential oncogenic and MLN4924-resistant effects of CSN5 on cervical cancer cells.
Zhang H, He P, Zhou Q, Lu Y, Lu B
Cancer cell international 2021 Jul 12;21(1):369
Cancer cell international 2021 Jul 12;21(1):369
Let-7i-5p Regulation of Cell Morphology and Migration Through Distinct Signaling Pathways in Normal and Pathogenic Urethral Fibroblasts.
Zhang K, Yang R, Chen J, Qi E, Zhou S, Wang Y, Fu Q, Chen R, Fang X
Frontiers in bioengineering and biotechnology 2020;8:428
Frontiers in bioengineering and biotechnology 2020;8:428
Nedd8-activating enzyme inhibitor MLN4924 (Pevonedistat), inhibits miR-1303 to suppress human breast cancer cell proliferation via targeting p27(Kip1).
Chen Y, Du M, Yusuying S, Liu W, Tan Y, Xie P
Experimental cell research 2020 Jul 15;392(2):112038
Experimental cell research 2020 Jul 15;392(2):112038
Gossypol inhibits cullin neddylation by targeting SAG-CUL5 and RBX1-CUL1 complexes.
Yu Q, Hu Z, Shen Y, Jiang Y, Pan P, Hou T, Pan ZQ, Huang J, Sun Y
Neoplasia (New York, N.Y.) 2020 Apr;22(4):179-191
Neoplasia (New York, N.Y.) 2020 Apr;22(4):179-191
Inhibition of neddylation causes meiotic arrest in mouse oocyte.
Yang M, Jin Y, Fan S, Liang X, Jia J, Tan Z, Huang T, Li Y, Ma T, Li M
Cell cycle (Georgetown, Tex.) 2019 Jun;18(11):1254-1267
Cell cycle (Georgetown, Tex.) 2019 Jun;18(11):1254-1267
The ubiquitin ligase SCF(FBXW7α) promotes GATA3 degradation.
Song N, Cao C, Tang Y, Bi L, Jiang Y, Zhou Y, Song X, Liu L, Ge W
Journal of cellular physiology 2018 Mar;233(3):2366-2377
Journal of cellular physiology 2018 Mar;233(3):2366-2377
Cullin-3-KCTD10-mediated CEP97 degradation promotes primary cilium formation.
Nagai T, Mukoyama S, Kagiwada H, Goshima N, Mizuno K
Journal of cell science 2018 Dec 12;131(24)
Journal of cell science 2018 Dec 12;131(24)
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12895-1-AP(CUL1 antibody) at dilution of 1:400
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-CUL1 (IP:12895-1-AP, 3ug; Detection:12895-1-AP 1:300) with HeLa cells lysate 2000ug.
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The CUL1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human CUL1. This antibody recognizes human,mouse,rat antigen. The CUL1 antibody has been validated for the following applications: ELISA, WB, IHC, IP analysis.