H00008454-D01P

antibody from Abnova Corporation

Targeting: CUL1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00008454-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00008454-D01P, RRID:AB_10552109

Product name

CUL1 purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human CUL1 protein.

Antigen sequence

MSSTRSQNPHGLKQIGLDQIWDDLRAGIQQVYTRQ
SMAKSRYMELYTHVYNYCTSVHQSNQARGAGVPPS
KSKKGQTPGGAQFVGLELYKRLKEFLKNYLTNLLK
DGEDLMDESVLKFYTQQWEDYRFSSKVLNGICAYL
NRHWVRRECDEGRKGIYEIYSLALVTWRDCLFRPL
NKQVTNAVLKLIEKERNGETINTRLISGVVQSYVE
LGLNEDDAFAKGPTLTVYKESFESQFLADTERFYT
RESTEFLQQNPVTEYMKKAEARLLEEQRRVQVYLH
ESTQDELARKCEQVLIEKHLEIFHTEFQNLLDADK
NEDLGRMYNLVSRIQDGLGELKKLLETHIHNQGLA
AIEKCGEAALNDPKMYVQTVLDVHKKYNALVMSAF
NNDAGFVAALDKACGRFINNNAVTKMAQSSSKSPE
LLARYCDSLLKKSSKNPEEAELEDTLNQVMVVFKY
IEDKDVFQKFYAKMLAKRLVHQNSASDDAEASMIS
KLKQACGFEYTSKLQRMFQDIGVSKDLNEQFKKHL
TNSEPLDLDFSIQVLSSGSWPFQQSCTFALPSELE
RSYQRFTAFYASRHSGRKLTWLYQLSKGELVTNCF
KNRYTLQASTFQMAILLQYNTEDAYTVQQLTDSTQ
IKMDILAQVLQILLKSKLLVLEDENANVDEVELKP
DTLIKLYLGYKNKKLRVNINVPMKTEQKQEQETTH
KNIEEDRKLLIQAAIVRIMKMRKVLKHQQLLGEVL
TQLSSRFKPRVPVIKKCIDILIEKEYLERVDGEKD
TYSYLA


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of CUL1 expression in transfected 293T cell line (H00008454-T02) by CUL1 MaxPab polyclonal antibody.Lane 1: CUL1 transfected lysate(89.70 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of purified MaxPab antibody to CUL1 on HeLa cell. [antibody concentration 30 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between CUL1 and FBXW11. HeLa cells were stained with anti-CUL1 rabbit purified polyclonal 1:1200 and anti-FBXW11 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)