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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
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- Product number
- PA5-95767 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cullin 3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Samples: HeLa, U-251MG, Jurkat, PC-12, Mouse brain, Mouse spleen, Rat brain; Cellular Location: Golgi apparatus, Nucleus Immunogen sequence: KEDGSEVGVG GAQVTGSNTR KHILQVSTFQ MTILMLFNNR EKYTFEEIQQ ETDIPERELV RALQSLACGK PTQRVLTKEP KSKEIENGHI FTVNDQFTSK LHRVKIQTVA AKQGESDPER KETRQKVDDD RKHEIEAAIV RIMKSRKKMQ HNVLVAEVTQ QLKARFLPSP VVIKKRIEGL IEREYLARTP EDRKVYTYVA
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.28 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western Blot analysis of Cullin 3 in extracts of various cell lines using Cullin 3 Polyclonal Antibody (Product # PA5-95767) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
