Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB100-58787 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-58787, RRID:AB_877705
- Product name
- Rabbit Polyclonal Cullin 3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Systematic identification of interactions between host cell proteins and E7 oncoproteins from diverse human papillomaviruses.
White EA, Sowa ME, Tan MJ, Jeudy S, Hayes SD, Santha S, Münger K, Harper JW, Howley PM
Proceedings of the National Academy of Sciences of the United States of America 2012 Jan 31;109(5):E260-7
Proceedings of the National Academy of Sciences of the United States of America 2012 Jan 31;109(5):E260-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Cullin 3 Antibody [NB100-58787] - Detection of Human Cul3 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Cul3 antibody NB100-58787 used for IP at 3 mcg/mg lysate. Cul3 was also immunoprecipitated by other rabbit anti-Cul3 antibodies NB100-58788 and NB100-58789, which recognize downstream epitopes. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cullin 3 Antibody [NB100-58787] - Section of human non-small cell lung cancer. Antibody: Affinity purified rabbit anti-Cul3 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB