PA1-84572

antibody from Invitrogen Antibodies

Targeting: GAD2 GAD65

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-84572

Provider

Invitrogen Antibodies

Product name

GAD65/GAD67 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

A suggested positive control for immunohistochemical applications is brain. In Western blot, a doublet at ~65/67 kDa has been observed.

References

Submitted references

Vascular amyloid accumulation alters the gabaergic synapse and induces hyperactivity in a model of cerebral amyloid angiopathy.

Cisternas P, Taylor X, Perkins A, Maldonado O, Allman E, Cordova R, Marambio Y, Munoz B, Pennington T, Xiang S, Zhang J, Vidal R, Atwood B, Lasagna-Reeves CA
Aging cell 2020 Oct;19(10):e13233

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-GAD65/GAD67 Polyclonal Antibody (Product # PA1-84572) and a 60 kDa band corresponding to GAD65/GAD67 was observed across tested samples. Whole cell extracts (40 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2), Mouse Kidney (Lane 3), Rat Kidney (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

4 FIGURE Decrease in the synaptic localization of inhibitory synaptic markers in Tg-FDD mice. (A) Immunofluorescence for GABA A receptor (green) and GAD (red) on cortex and hippocampus. (B) Quantification of the number of merge puncta for both markers on A. (C) Double staining for GABA B receptor (green) and GAD (red). (D) Quantification of the number of merged puncta for both markers on C. p < 0.05 indicated on each graph, Mann-Whitney test, n = 9 photographs from 3 different animals per genotype. Data represented as mean +- SEM. Scale bar 10 mum.