20746-1-AP

antibody from Proteintech Group

Targeting: GAD2 GAD65

Provider product page for 20746-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Antibody data

Antibody Data
Antigen structure
References [26]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]

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Product number: 20746-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#20746-1-AP, RRID:AB_10949196
Product name: GAD65 antibody
Antibody type: Polyclonal
Description: GAD65 antibody (Cat. #20746-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

GAD2 protein structure - 20746-1-AP shown in red.

Submitted references The Hypothalamic Medial Preoptic Area-Paraventricular Nucleus Circuit Modulates Depressive-Like Behaviors in a Mouse Model of Postpartum Depression.

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Research (Washington, D.C.) 2025;8:0701

Effect of Voluntary Wheel Running on Anxiety- and Depression-Like Behaviors in Fluoride-Exposed Mice.

Qi M, Wu Y, Shi H, Liu J, Zhu R, Wang J, Rafique A, Yang B, Niu R, Zhang D, Sun Z
Biological trace element research 2025 Jul;203(7):3724-3741

Evaluation of TRPM2 Channel-Mediated Autophagic Signaling Pathway in Hippocampus and Cortex Tissues of Rat Offspring Following Prenatal Exposure to Elevated Alcohol Levels.

Uğuz AC, Okan A, Doğanyiğit Z, Yilmaz S, Ateş Ş, Arikan Söylemez ES, Karabulut S, Kumru AS, Espino J
Environmental toxicology 2025 Feb;40(2):222-244

GABA regulates metabolic reprogramming to mediate the development of brain metastasis in non-small cell lung cancer.

Xie M, Qin H, Liu L, Wu J, Zhao Z, Zhao Y, Fang Y, Yu X, Su C
Journal of experimental & clinical cancer research : CR 2025 Feb 19;44(1):61

High-fat diet disrupts a septal control on feeding to promote obesity in male mice.

Jiang S, Lai S, Jing H, Wu X, Li F, Chen B, Bao J, Wang L, Chen G, Zhu Y
Nature communications 2025 Dec 27;17(1):1248

Non-invasive light flickering reinstates visual plasticity in adult mice via lipocalin 2.

Ma C, Wang Z, Wu W, Ma X, Wang L, Zhang X, Chen W, Kuang X, Li Y, Miao Y, Xue T, Jiang B, Yang Y
BMC biology 2025 Aug 4;23(1):237

Transcriptomic Analysis of Immune Tolerance Induction in NOD Mice Following Oral Vaccination with GAD65-Lactococcus lactis.

Xie M, Ma C, Wang X, Li T, Zhang S, Shi J, Sun J, Hu Y
Vaccines 2025 Aug 30;13(9)

Prenatal LPS leads to increases in RAS expression within the PVN and overactivation of sympathetic outflow in offspring rats.

Hao X, Long X, Fan L, Gou J, Liu Y, Fu Y, Zhao H, Xie X, Wang D, Liang G, Ye Y, Wang J, Li S, Zeng C
Hypertension research : official journal of the Japanese Society of Hypertension 2024 Sep;47(9):2363-2376

Paeonol and glycyrrhizic acid in combination ameliorate the recurrent nitroglycerin-induced migraine-like phenotype in rats by regulating the GABBR2/TRPM8/PRKACA/TRPV1 pathway.

Zhang Y, Ge F, Luo Y, Ji X, Liu Z, Qiu Y, Hou J, Zhou R, Zhao C, Xu Q, Zhang S, Yu X, Wang C, Ge D, Meng F, Tao X
Journal of ethnopharmacology 2024 Nov 15;334:118464

Constraint-Induced Movement Therapy Modulates Neuron Recruitment and Neurotransmission Homeostasis of the Contralesional Cortex to Enhance Function Recovery after Ischemic Stroke.

Zhang A, Xing Y, Zheng J, Li C, Hua Y, Hu J, Tian Z, Bai Y
ACS omega 2024 May 14;9(19):21612-21625

The effect of modified Qiyuan paste on mice with low immunity and sleep deprivation by regulating GABA nerve and immune system.

Rong M, Jia JJ, Lin MQ, He XL, Xie ZY, Wang N, Zhang ZH, Dong YJ, Xu WF, Huang JH, Li B, Jiang NH, Lv GY, Chen SH
Chinese medicine 2024 Jun 12;19(1):84

Metabolic reprogramming driven by EZH2 inhibition depends on cell-matrix interactions.

W-M Fan T, Islam JMM, Higashi RM, Lin P, Brainson CF, Lane AN
The Journal of biological chemistry 2024 Jan;300(1):105485

Integrin α3 is required for high-frequency repetitive transcranial magnetic stimulation-induced glutamatergic synaptic transmission in mice with ischemia.

Liu L, Hu H, Wu J, Koleske AJ, Chen H, Wang N, Yu K, Wu Y, Xiao X, Zhang Q
CNS neuroscience & therapeutics 2024 Apr;30(4):e14498

Cancer-cell-derived GABA promotes β-catenin-mediated tumour growth and immunosuppression.

Huang D, Wang Y, Thompson JW, Yin T, Alexander PB, Qin D, Mudgal P, Wu H, Liang Y, Tan L, Pan C, Yuan L, Wan Y, Li QJ, Wang XF
Nature cell biology 2022 Feb;24(2):230-241

Gold Nanostrip Array-Mediated Wireless Electrical Stimulation for Accelerating Functional Neuronal Differentiation.

Yang H, Su Y, Sun Z, Ma B, Liu F, Kong Y, Sun C, Li B, Sang Y, Wang S, Li G, Qiu J, Liu C, Geng Z, Liu H
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2022 Aug;9(22):e2202376

Intrathecally administered pizotifen alleviates neuropathic and inflammatory pain in mice by enhancing GABAergic inhibition.

Li J, Zhang X, Xu C, Liu YX, Ge C, Zhao Z, Zhu YB, Bao HG
Neuroscience letters 2022 Apr 1;775:136545

Proteomics Study Reveals the Anti-Depressive Mechanisms and the Compatibility Advantage of Chaihu-Shugan-San in a Rat Model of Chronic Unpredictable Mild Stress.

Zhu X, Li T, Hu E, Duan L, Zhang C, Wang Y, Tang T, Yang Z, Fan R
Frontiers in pharmacology 2021;12:791097

BNST(AV) (GABA)-PVN(CRF) Circuit Regulates Visceral Hypersensitivity Induced by Maternal Separation in Vgat-Cre Mice.

Huang ST, Song ZJ, Liu Y, Luo WC, Yin Q, Zhang YM
Frontiers in pharmacology 2021;12:615202

A diet high in sugar and fat influences neurotransmitter metabolism and then affects brain function by altering the gut microbiota.

Guo Y, Zhu X, Zeng M, Qi L, Tang X, Wang D, Zhang M, Xie Y, Li H, Yang X, Chen D
Translational psychiatry 2021 May 27;11(1):328

Maternal high sugar and fat diet benefits offspring brain function via targeting on the gut-brain axis.

Wang D, Zhang H, Zeng M, Tang X, Zhu X, Guo Y, Qi L, Xie Y, Zhang M, Chen D
Aging 2021 Mar 26;13(7):10240-10274

A five-m6A regulatory gene signature is a prognostic biomarker in lung adenocarcinoma patients.

Wu X, Sheng H, Wang L, Xia P, Wang Y, Yu L, Lv W, Hu J
Aging 2021 Mar 26;13(7):10034-10057

Spinal GABAergic neurons are under feed-forward inhibitory control driven by Aδ and C fibers in Gad2 td-Tomato mice.

Liu P, Zhang X, He X, Jiang Z, Wang Q, Lu Y
Molecular pain 2021 Jan-Dec;17:1744806921992620

A sex-dependent delayed maturation of visual plasticity induced by adverse experiences in early childhood.

Liu Y, Wang Z, Zhang X, Li S, Wu W, Li X, Yang Y
Neurobiology of stress 2020 Nov;13:100256

Prenatal cold exposure causes hypertension in offspring by hyperactivity of the sympathetic nervous system.

Chen K, Sun D, Qu S, Chen Y, Wang J, Zhou L, Jose PA, Yang Y, Zeng C
Clinical science (London, England : 1979) 2019 May 31;133(9):1097-1113

Renal Denervation Improves the Baroreflex and GABA System in Chronic Kidney Disease-induced Hypertension.

Chen HH, Cheng PW, Ho WY, Lu PJ, Lai CC, Tseng YM, Fang HC, Sun GC, Hsiao M, Liu CP, Tseng CJ
Scientific reports 2016 Dec 5;6:38447

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Molecular brain 2015 Aug 19;8:49

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: mouse brain tissue were subjected to SDS PAGE followed by western blot with 20746-1-AP(GAD2 antibody) at dilution of 1:1000
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The GAD2 antibody from Proteintech is a rabbit polyclonal antibody to a peptide of human GAD2. This antibody recognizes human,mouse,rat antigen. The GAD2 antibody has been validated for the following applications: ELISA, WB, IF analysis.
Appendix: 20746-1-AP.pdf

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