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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MA1-25289 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- APOE Monoclonal Antibody (1D2)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Predicted molecular weight is 38 kDa.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1D2
- Vial size
- 1 mL
- Concentration
- Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of APOE was performed using 70% confluent log phase Hep G2 cells and Hep G2 cells treated with PTI. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with APOE Mouse Monoclonal Antibody (1D2) (Product # MA1-25289) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32723) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents Hep G2 untreated cells showing no expression of APOE. Panel f represents A-431 cells treated with PTI showing no expression of APOE. Panel g represents A-431 untreated cells showing no expression of APOE. Panel h represents control Hep G2 cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of 8.6 week placenta tissue using (Product # MA1-25289) APOE Monoclonal Antibody (1D2).
