Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
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Validation data
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- Product number
- AF4144 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Apolipoprotein E/ApoE Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Apolipoprotein E/ApoE in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Serum Apolipoprotein E and Other Inflammatory Markers Can Identify Non-Responding Patients to a Dendritic Cell Vaccine.
MicroRNA-27a regulates lipid metabolism and inhibits hepatitis C virus replication in human hepatoma cells.
Leeman H, Kaminska E, Green D, Bodman-Smith M, Gravett A, Bodman-Smith K, Copier J, Coulton G, Fusi A, Dalgleish AG
Translational oncology 2019 Mar;12(3):397-403
Translational oncology 2019 Mar;12(3):397-403
MicroRNA-27a regulates lipid metabolism and inhibits hepatitis C virus replication in human hepatoma cells.
Shirasaki T, Honda M, Shimakami T, Horii R, Yamashita T, Sakai Y, Sakai A, Okada H, Watanabe R, Murakami S, Yi M, Lemon SM, Kaneko S
Journal of virology 2013 May;87(9):5270-86
Journal of virology 2013 May;87(9):5270-86
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Apolipoprotein E/ApoE by Western Blot. Western blot shows lysates of human plasma. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Apolipoprotein E/ApoE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4144) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Apolipoprotein E/ApoE at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Apolipoprotein E/ ApoE by Western Blot. Western blot shows conditioned media from SK-Mel-28 human malignant melanoma cell line, HepG2 human hepatocellular carcinoma cell line, and human peripheral blood mononuclear cells (PBMC). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Apolipoprotein E/ApoE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4144) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Apolipoprotein E/ApoE at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Apolipoprotein E/ ApoE by Simple WesternTM. Simple Western lane view shows human plasma, loaded at 1:10 dilution. A specific band was detected for Apolipoprotein E/ApoE at approximately 39 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Apolipoprotein E/ApoE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4144) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.