PB9327

antibody from Boster Biological Technology

Targeting: APOE AD2

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

PB9327

Provider

Boster Biological Technology

Product name

Anti-Apolipoprotein E/APOE Antibody Picoband™

Antibody type

Polyclonal

Description

Polyclonal antibody for Apolipoprotein E/APOE detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, IHC-P, IHC-F, FCM, ELISA(Cap). Reactive species: Human. Apolipoprotein E/APOE information: Molecular Weight: 36154 MW; Subcellular Localization: Secreted; Tissue Specificity: Occurs in all lipoprotein fractions in plasma. It constitutes 10-20% of very low density lipoproteins (VLDL) and 1-2% of high density lipoproteins (HDL). APOE is produced in most organs. Significant quantities are produced in liver, brain, spleen, lung, adrenal, ovary, kidney and muscle.

Reactivity

Human

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

Phaseolus vulgaris Erythroagglutinin (PHA-E)-Positive Ceruloplasmin Acts as a Potential Biomarker in Pancreatic Cancer Diagnosis.

Sha S, Wang Y, Liu M, Liu G, Fan N, Li Z, Dong W
Cells 2022 Aug 8;11(15)

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Identification of Apolipoprotein E as a Potential Diagnostic Biomarker of Nasopharyngeal Carcinoma.

Xue Y, Huang S, Huang J, Li S, Zhang C, Zhou X
Cancer management and research 2020;12:8943-8950

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of Apolipoprotein E using anti-Apolipoprotein E antibody (PB9327). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Human Placenta Tissue Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Apolipoprotein E antigen affinity purified polyclonal antibody (Catalog # PB9327) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Apolipoprotein E at approximately 36KD. The expected band size for Apolipoprotein E is at 36KD.

Additional image