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ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- NB400-158 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB400-158, RRID:AB_10001452
- Product name
- Goat Polyclonal Apolipoprotein E/ApoE Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA.This reacts with human apoLipoprotein E and has negligible cross-reactivity with Type A-I, A-II, B, C-I, C-II, C-III and J apoLipoproteins.
- Reactivity
- Human, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
Submitted references An altered pattern of circulating apolipoprotein E3 isoforms is implicated in preeclampsia.
Atkinson KR, Blumenstein M, Black MA, Wu SH, Kasabov N, Taylor RS, Cooper GJ, North RA, SCOPE Consortium.
Journal of lipid research 2009 Jan;50(1):71-80
Journal of lipid research 2009 Jan;50(1):71-80
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Apolipoprotein E/ApoE Antibody [NB400-158] - Lane 1: rat glial whole cell lysate. Load: 40 ug per lane. Primary antibody: Apolipoprotein E antibody at 1:1000 for overnight at 4C. Secondary antibody: HRP rabbit secondary antibody at 1:200 for 45 min at RT. Block: 3%BLOTTO overnight at 4C. Predicted/Observed size: 36 kDa, 35-36 kDa for APO-E. MW markers arrows at 25kDa and 37kDa. Other band(s): 55kDa.
