M00015

antibody from Boster Biological Technology

Targeting: APOE AD2

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M00015

Provider

Boster Biological Technology

Product name

Anti-Apolipoprotein E APOE Monoclonal Antibody

Antibody type

Monoclonal

Description

Rabbit IgG Monoclonal antibody for Apolipoprotein E detection. Tested positive for WB, IHC, ICC/IF, IP in Human, Mouse.

Reactivity

Human, Mouse

Host

Rabbit

Antibody clone number

AbA15

Vial size

100ug

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of APOE using anti-APOE antibody (M00015) on paraffin-embedded human kidney.APOE was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-APOE Antibody (M00015) overnight at 4°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image