MA5-15852

antibody from Invitrogen Antibodies

Targeting: APOE AD2

ELISA

Antibody data

Submit

Validation data

Product number: MA5-15852 - Provider product page
Provider: Invitrogen Antibodies
Product name: APOE Monoclonal Antibody (1H4)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA5-15852 targets ApoE in indirect ELISA, FACS, IHC, and WB applications and shows reactivity with Human samples. The MA5-15852 immunogen is purified recombinant fragment of human ApoE expressed in E. Coli. . MA5-15852 detects ApoE which has a predicted molecular weight of approximately 36kDa.
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: 1H4
Vial size: 100 µL
Concentration: Conc. Not Determined
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

APOE protein structure - MA5-15852 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of ApoE using ApoE monoclonal antibody (Product # MA5-15852) in HEK293 (1) and ApoE (AA: 20-267) human IgG Fc transfected HEK293 (2) cell lysate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of ApoE using ApoE monoclonal antibody (Product # MA5-15852) in HEK293 (1) and ApoE (AA: 20-267) human IgG Fc transfected HEK293 (2) cell lysate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of ApoE using ApoE monoclonal antibody (Product # MA5-15852) in HEK293 (1) and ApoE (AA: 20-267) human IgG Fc transfected HEK293 (2) cell lysate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-APOE Monoclonal Antibody (1H4) (Product # MA5-15852) and a 36 kDa band corresponding to APOE was observed in Hep G2 and Caco-2 with PTI treatment but was absent in A-431 and Daudi which are reported to be negative. A non-specific band around 22 kDa has also been observed in Hep G2 and Caco-2. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), Hep G2 treated with Protein Transport Inhibitor (PTI; 1x for 4 hours) (Lane 2), Caco-2 (Lane 3), Caco-2 treated with Protein Transport Inhibitor (PTI; 1x for 4 hours) (Lane 4), A-431 (Lane 5), A-431 treated with Protein Transport Inhibitor (PTI; 1x for 4 hours) (Lane 6), Daudi (Lane 7) and Daudi treated with Protein Transport Inhibitor (1x for 4 hours) (Lane 8) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).