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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
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- Product number
- MA5-16146 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- APOE Monoclonal Antibody (4E4)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4E4
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Ageing related thyroid deficiency increases brain-targeted transport of liver-derived ApoE4-laden exosomes leading to cognitive impairment.
Zhang M, Gong W, Zhang D, Ji M, Chen B, Chen B, Li X, Zhou Y, Dong C, Wen G, Zhan X, Wu X, Cui L, Feng Y, Wang S, Yuan H, Xu E, Xia M, Verkhratsky A, Li B
Cell death & disease 2022 Apr 25;13(4):406
Cell death & disease 2022 Apr 25;13(4):406
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ApoE4 expression in concentrated supernatants of CHO cells secreting human ApoE2, ApoE3 or ApoE4 using APOE Monoclonal Antibody (Product # MA5-16146). Image courtesy of Dr. Marko Roblek from the Medical University of Vienna.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of APOE was performed using 70% confluent log phase HepG2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with APOE Mouse Monoclonal Antibody (Product # MA5-16146) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockout of APOE was achieved by CRISPR-Cas9 genome editing. Immunofluorescence analysis was performed on HEPG2 wild type control cells treated with Protein Transport Inhibitor (PTI) (1X for 4hr) (panels a,c) and HEPG2 APOE KO cells treated with Protein Transport Inhibitor (PTI) (1X for 4hr) (panel b,d). Cells were fixed, permeabilized, and labelled with APOE Monoclonal Antibody (Product # MA5-16146) at a concentration of 82032 µg/mL. Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962), and Rhodamine Phalloidin (Product # R415) at a dilution of 1:300 was used for cytoskeletal F-actin (red) staining. Loss of signal (panel b, d) upon CRISPR mediated knockout (KO) confirms that antibody is specific to APOE (green). The images were captured at 60X magnification.
