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- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- MAB41441 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Apolipoprotein E/ApoE Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human ApoE in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) ApoA1 or rhApoA2 is observed.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P02649- Isotype
- IgG
- Antibody clone number
- 395004
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Serum Apolipoprotein E and Other Inflammatory Markers Can Identify Non-Responding Patients to a Dendritic Cell Vaccine.
Effect of human very low-density lipoproteins on cardiotrophin-like cytokine factor 1 (CLCF1) activity.
Leeman H, Kaminska E, Green D, Bodman-Smith M, Gravett A, Bodman-Smith K, Copier J, Coulton G, Fusi A, Dalgleish AG
Translational oncology 2019 Mar;12(3):397-403
Translational oncology 2019 Mar;12(3):397-403
Effect of human very low-density lipoproteins on cardiotrophin-like cytokine factor 1 (CLCF1) activity.
Pasquin S, Chehboun S, Dejda A, Meliani Y, Savin V, Warner GJ, Bosse R, Tormo A, Mayer G, Sharma M, Sapieha P, Martel C, Gauchat JF
Scientific reports 2018 Mar 5;8(1):3990
Scientific reports 2018 Mar 5;8(1):3990
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Detection of Human Apolipoprotein E/ApoE by Western Blot. Western blot shows lysates of human lung tissue and human ovary tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human Apolipoprotein E/ApoE Monoclonal Antibody (Catalog # MAB41441) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Apolipoprotein E/ApoE at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
