MA1-20832

antibody from Invitrogen Antibodies

Targeting: CENPA CenH3, CENP-A

Western blot

Blocking/Neutralizing

Antibody data

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Validation data

Product number: MA1-20832 - Provider product page
Provider: Invitrogen Antibodies
Product name: CENPA Monoclonal Antibody (3-19)
Antibody type: Monoclonal
Antigen: Synthetic peptide
Description: Recommended positive controls: , BE cells. This antibody does not cross react with mouse CENPA proteins. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Chicken/Avian
Host: Mouse
Isotype: IgG
Antibody clone number: 19-Mar
Vial size: 50 µg
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

CENPA protein structure - MA1-20832 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of CENPA in human metaphase chromosomes using a CENPA monoclonal antibody (Product # MA1-20832) followed by detection using a goat anti-mouse secondary antibody (green).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of CENPA was performed using 70% confluent log phase HT-29 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CENPA Monoclonal Antibody (3-19) (Product # MA1-20832) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Centromere localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of CENPA was performed using 70% confluent log phase HT-29 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CENPA Monoclonal Antibody (3-19) (Product # MA1-20832) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Centromere localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.