Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA1-20832 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CENPA Monoclonal Antibody (3-19)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: , BE cells.
- Antibody clone number
- 19-Mar
- Concentration
- 1 mg/mL
Submitted references Inheritance of CENP-A Nucleosomes during DNA Replication Requires HJURP.
Zasadzińska E, Huang J, Bailey AO, Guo LY, Lee NS, Srivastava S, Wong KA, French BT, Black BE, Foltz DR
Developmental cell 2018 Nov 5;47(3):348-362.e7
Developmental cell 2018 Nov 5;47(3):348-362.e7
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Jurkat (1), Raji (2), PC12 (3), Rat-1 (4), WR19L (5), C2C12 (6), and NIH3T3 (7) cell lysates probed with CENPA Monoclonal Antibody (3-19) (Product # MA1-20832).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CENPA Monoclonal Antibody (3-19) (Product # MA1-20832). Hela cells (human), Friend cells (mouse) and calf thymus. 5 g acid-extracted histones were loaded per lane. Probed with CENPA Monoclonal Antibody (3-19) (Product # MA1-20832). Images taken following 5 min exposure. 1:1,000 dilution. Lane 1: Friend cells (mouse); Lane 2: Hela cells (human); Lane 3: Calf thymus.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Jurkat (1), Raji (2), PC12 (3), Rat-1 (4), WR19L (5), C2C12 (6), and NIH3T3 (7) cell lysates probed with CENPA Monoclonal Antibody (3-19) (Product # MA1-20832).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of CENPA was achieved by transfecting HT-29 with CENPA specific siRNAs (Silencer® select Product # S2907, S2906). Western blot analysis (Fig. a) was performed using Nuclear enriched extracts from the CENPA knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with CENPA Monoclonal Antibody (3-19) (Product # MA1-20832, 1 ug/ml ) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to CENPA.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-CENPA Monoclonal Antibody (3-19) (Product # MA1-20832) and a 17kDa band corresponding to CENPA was observed across all the cell lines tested. Nuclear enriched extracts (30 µg lysate) of HCT 116 (Lane 1), SW480 (Lane 2), HT-29 (Lane 3), Jurkat (Lane 4) and IMR32 (Lane 5) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0342BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 ug/ml) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CENPA in human metaphase chromosomes using a CENPA monoclonal antibody (Product # MA1-20832) followed by detection using a goat anti-mouse secondary antibody (green).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of CENPA was performed using 70% confluent log phase HT-29 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CENPA Monoclonal Antibody (3-19) (Product # MA1-20832) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Centromere localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical detection of CENP-A on paraffin embedded section of a squamous epithelium of human tonsil using CENPA Monoclonal Antibody (3-19) (Product # MA1-20832).
